DOPA Decarboxylase antibody

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Rabbit anti Bovine DOPA Decarboxylase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1545 WB 0.1 ml
Rabbit anti Bovine DOPA decarboxylase antibody recognizes DOPA decarboxylase, also known as aromatic-L-amino acid decarboxylase or DDC), an enzyme that catalyses the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. The enzyme is expressed ubiquitously and is essential for the formation of catecholamines, indoleamines and trace amines. DDC is considered to be the rate-limiting step for the formation of trace amines, but not for the formation of catecholamines or indoleamines. It also becomes rate-limiting for dopamine formation in Parkinson's disease patients treated with L-DOPA.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to DOPA Decarboxylase receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
SDS denatured, recombinant bovine DOPA decarboxylase expressed E. coli and purified from inclusion bodies.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P27718 Related reagents
Entrez Gene
DDC Related reagents
GO Terms
GO:0004058 aromatic-L-amino-acid decarboxylase activity
GO:0019752 carboxylic acid metabolic process
GO:0030170 pyridoxal phosphate binding
GO:0042423 catecholamine biosynthetic process
Regulatory
For research purposes only

Applications of DOPA Decarboxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1545 detects a band of approximately 55kDa in rat adrenal medulla.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*