DOPA Decarboxylase antibody

Rabbit anti Bovine DOPA Decarboxylase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
DOPA Decarboxylase

Product Code Applications Pack Size List Price Your Price Qty
AHP1545
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
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Rabbit anti Bovine DOPA decarboxylase antibody recognizes DOPA decarboxylase, also known as aromatic-L-amino acid decarboxylase or DDC), an enzyme that catalyses the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. The enzyme is expressed ubiquitously and is essential for the formation of catecholamines, indoleamines and trace amines. DDC is considered to be the rate-limiting step for the formation of trace amines, but not for the formation of catecholamines or indoleamines. It also becomes rate-limiting for dopamine formation in Parkinson's disease patients treated with L-DOPA.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to DOPA Decarboxylase receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
SDS denatured, recombinant bovine DOPA decarboxylase expressed E. coli and purified from inclusion bodies.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1545 detects a band of approximately 55kDa in rat adrenal medulla.

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Synonyms
AROMATIC-L-AMINO-ACID Decarboxylase
RRID
AB_1102526
UniProt
P27718
Entrez Gene
DDC
GO Terms
GO:0004058 aromatic-L-amino-acid decarboxylase activity
GO:0019752 carboxylic acid metabolic process
GO:0030170 pyridoxal phosphate binding
GO:0042423 catecholamine biosynthetic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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