CXCL9 antibody

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Rabbit anti Bovine CXCL9:Biotin

Rabbit anti Bovine CXCL9

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2369B
Datasheet
E WB
MSDS
50 µg loader

AHP2369
Datasheet
E WB
MSDS
0.1 mg loader

Rabbit anti Bovine CXCL9 polyclonal antibody recognizes bovine CXCL9, otherwise known as MIG (monokine induced by Interferon-gamma). CXCL9 is a member of the C-X-C family of chemokine receptors, secreted by interferonγ stimulated monocytes, macrophages (Muller et al. 2010) and endothelial cells (Loos et al. 2006).

CXCL9 acts as a Th1 (type 1 helper T) cell chemoattractant, and plays a role in the growth, activation and movement of cells associated with, and in, tumour growth inhibition and angiogenesis (Whiting et al. 2004).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine CXCL9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant bovine CXCL9
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
A9QWP9 Related reagents
Entrez Gene
CXCL9 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of CXCL9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.01 ug/ml 0.2 ug/ml
Western Blotting 0.1 ug/ml 1.0 ug/ml
ELISA 1.0 ug/ml 10 ug/ml
Western Blotting 0.1 ug/ml 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with AHP2369 as the capture reagent. Recombinant bovine CXCL9 (PBP023) may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2369B as the detection reagent. Recombinant Bovine CXCL9 (PBP023) may be used as a standard.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Bovine CXCL9 AHP2369 E WB 0.1 mg loader
Recombinant Bovine CXCL9 PBP023 E 5 µg loader
Rabbit anti Bovine CXCL9:Biotin AHP2369B E WB 50 µg loader
Recombinant Bovine CXCL9 PBP023 E 5 µg loader

Product Specific References

Further Reading

  1. Müller, M. et al. (2010) Review: The chemokine receptor CXCR3 and its ligands CXCL9, CXCL10 and CXCL11 in neuroimmunity--a tale of conflict and conundrum.
    Neuropathol Appl Neurobiol. 36: 368-87.
  2. Whiting, D. et al. (2004) Chemokine monokine induced by IFN-gamma/CXC chemokine ligand 9 stimulates T lymphocyte proliferation and effector cytokine production.
    J Immunol. 172: 7417-24.
  3. Loos, T. et al. (2006) TLR ligands and cytokines induce CXCR3 ligands in endothelial cells: enhanced CXCL9 in autoimmune arthritis.
    Lab Invest. 86: 902-16.