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Asialofetuin antibody

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Sheep anti Bovine Asialofetuin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0754-8050 E 1 ml
Sheep anti Bovine asialofetuin antibody recognizes bovine Alpha-2-HS-glycoprotein, also known as asialofetuin or fetuin-A. Asialofetuin is a 359 amino acid ~55kDa secreted glycoprotein containing two cystatin fetuin-A-type domains.

Asialofetuin is produced in the liver and bone marrow and acts as a negative acute-phase protein attenuating the inflammatory response to acute injury (Wang et al. 2010).

In a simple antigen down ELISA assay Sheep anti Bovine asialofetuin antibody reacts with:
asialofetuin (ab at 1/8000) 100%
fetuin (ab at 1/2000) 100%
fetuin (ab at 1/8000) 50%

Product Details

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Native
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12763 Related reagents
Entrez Gene
AHSG Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005576 extracellular region
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0030502 negative regulation of bone mineralization
GO:0050727 regulation of inflammatory response
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of Asialofetuin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Asialofetuin antibody

  1. Blome, M.C. et al. (2010) Surface plasmon resonance analysis of ricin binding to plasma membranes isolated from NIH 3T3 cells.
    Anal Biochem. 396: 212-6.
  2. Bayer, H. et al. (2012) Evaluation of riproximin binding properties reveals a novel mechanism for cellular targeting.
    J Biol Chem. 287 (43): 35873-86.
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