Asialofetuin antibody
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Sheep anti Bovine asialofetuin antibody recognizes bovine Alpha-2-HS-glycoprotein, also known as asialofetuin or fetuin-A. Asialofetuin is a 359 amino acid ~55kDa secreted glycoprotein containing two cystatin fetuin-A-type domains.
Asialofetuin is produced in the liver and bone marrow and acts as a negative acute-phase protein attenuating the inflammatory response to acute injury (Wang et al. 2010).
In a simple antigen down ELISA assay Sheep anti Bovine asialofetuin antibody reacts with:
asialofetuin (ab at 1/8000) 100%
fetuin (ab at 1/2000) 100%
fetuin (ab at 1/8000) 50%
Asialofetuin is produced in the liver and bone marrow and acts as a negative acute-phase protein attenuating the inflammatory response to acute injury (Wang et al. 2010).
In a simple antigen down ELISA assay Sheep anti Bovine asialofetuin antibody reacts with:
asialofetuin (ab at 1/8000) 100%
fetuin (ab at 1/2000) 100%
fetuin (ab at 1/8000) 50%
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P12763
- Entrez Gene
- AHSG
- GO Terms
- GO:0006953 acute-phase response
- GO:0005576 extracellular region
- GO:0004869 cysteine-type endopeptidase inhibitor activity
- GO:0030502 negative regulation of bone mineralization
- GO:0050727 regulation of inflammatory response
- GO:0050766 positive regulation of phagocytosis
- Regulatory
- For research purposes only
Applications of Asialofetuin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/4000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Asialofetuin antibody
-
Blome, M.C. et al. (2010) Surface plasmon resonance analysis of ricin binding to plasma membranes isolated from NIH 3T3 cells.
Anal Biochem. 396: 212-6. -
Bayer, H. et al. (2012) Evaluation of riproximin binding properties reveals a novel mechanism for cellular targeting.
J Biol Chem. 287 (43): 35873-86.
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