IL-1 Beta antibody

Rabbit anti Chicken Interleukin-1 beta:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-1 Beta

Product Code Applications Pack Size List Price Your Price Qty
AHP941Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Chicken interleukin-1 beta antibody recognizes chicken Interleukin 1 beta (chIL-1beta), which shares homology with mammalian IL-1beta.

IL-1 is a major regulator in both innate and adaptive immune responses, plays an important role in the regulation of acute inflammation and has also been implicated in many other processes, including both fever and fibrinogen induction. The previous names by which IL-1 has been known includes lymphocyte activating factor, endogenous pyrogen, catabolin, hemopoietin-1, melanoma growth inhibition factor, and osteoclast activating factor, reflecting its widespread cell expression.

Chicken models indicate physiological similarities between chIL-1beta and mammalian IL-1beta in relation to its neural distribution and several factors have been shown to induce chIL-1beta secretion, including protein kinase C (PKC), Calmodulin-dependent protein kinase, lipopolysaccharide, phorbol esters, calcium and silica.

Rabbit anti Chicken interleukin-1 beta antibody (AHP941Z) neutralizes chicken IL-1 beta (Gyorfy et al. 2003).

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interleukin-1β were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP941Z detects a band of approximately 29kDa in HD-11 cell lysates transfected with chIL-1.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for IL-1 Beta antibody

  1. Gyorfy, Z. et al. (2003) Truncated chicken interleukin-1beta with increased biologic activity.
    J Interferon Cytokine Res. 23 (5): 223-8.
  2. De Boever, S. et al. (2010) Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.
    Avian Pathol. 39: 41-6.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Weining, K.C. et al. (1998) A chicken homolog of mammalian interleukin-1 beta: cDNA cloning and purification of active recombinant protein.
    Eur J Biochem. 258 (3): 994-1000.
  3. Bombara, C.J. & TaylorRl, J.r. (1991) Signal transduction events in chicken interleukin-1 production.
    Poult Sci. 70 (6): 1372-80.

RRID
AB_567064
UniProt
O73909
View more products with IL-1 BETA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Chicken Products