IL-1 Beta antibody

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Rabbit anti Chicken Interleukin-1 beta:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP941Z E FN WB 0.5 mg
Rabbit anti Chicken interleukin-1 beta antibody recognizes chicken Interleukin 1 beta (chIL-1beta), which shares homology with mammalian IL-1beta.

IL-1 is a major regulator in both innate and adaptive immune responses, plays an important role in the regulation of acute inflammation and has also been implicated in many other processes, including both fever and fibrinogen induction. The previous names by which IL-1 has been known includes lymphocyte activating factor, endogenous pyrogen, catabolin, hemopoietin-1, melanoma growth inhibition factor, and osteoclast activating factor, reflecting its widespread cell expression.

Chicken models indicate physiological similarities between chIL-1beta and mammalian IL-1beta in relation to its neural distribution and several factors have been shown to induce chIL-1beta secretion, including protein kinase C (PKC), Calmodulin-dependent protein kinase, lipopolysaccharide, phorbol esters, calcium and silica.

Rabbit anti Chicken interleukin-1 beta antibody (AHP941Z) neutralizes chicken IL-1 beta (Gyorfy et al. 2003).

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interleukin-1β were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant chicken IL-1β
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O73909 Related reagents
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP941Z detects a band of approximately 29kDa in HD-11 cell lysates transfected with chIL-1.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for IL-1 Beta antibody

  1. Gyorfy, Z. et al. (2003) Truncated chicken interleukin-1beta with increased biologic activity.
    J Interferon Cytokine Res. 23 (5): 223-8.
  2. De Boever, S. et al. (2010) Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.
    Avian Pathol. 39: 41-6.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Weining, K.C. et al. (1998) A chicken homolog of mammalian interleukin-1 beta: cDNA cloning and purification of active recombinant protein.
    Eur J Biochem. 258 (3): 994-1000.
  3. Bombara, C.J. & TaylorRl, J.r. (1991) Signal transduction events in chicken interleukin-1 production.
    Poult Sci. 70 (6): 1372-80.