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Tetanus Exotoxin antibody

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Goat anti Clostridium tetani Tetanus Exotoxin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Tetanus Exotoxin

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Product Code Applications Pack Size List Price Your Price Qty
8750-2059G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 1 ml
List Price Your Price

Goat anti tetanus exotoxin antibody recognizes tetanus toxin, also known as tentoxylysin, produced by the soil micro-organism Clostridium tetani. Infection by Clostridium tetani. results in its systemic growth and secretion of tetanus toxin which causes muscle spasms by inhibiting neurotransmitter release.

Goat anti tetanus exotoxin antibody also reacts with the C-fragment (~50 kDa carboxy terminal residues 865-1315) of tetanus toxin (Emsley et al. 2000).

Target Species
Bacterial
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to C.Tetani Exotoxin were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Tetanus Exotoxin.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Tetanus Exotoxin antibody

  1. Paillot, R.et al. (2015) ISCOM-based equine influenza vaccine: Duration of immunity and randomised clinical trials to assess an accelerated schedule of immunisation and efficacy
    Trials in Vaccinology. 4: 61-70.

Further Reading

  1. Cook, T.M. et al. (2001) Tetanus: a review of the literature.
    Br J Anaesth. 87 (3): 477-87.

RRID
AB_808753
UniProt
P04958
GO Terms
GO:0004222 metalloendopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0009405 pathogenesis
GO:0050827 toxin receptor binding
GO:0051609 inhibition of neurotransmitter uptake

8750-2059G

150357 150359 158955 1808

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