Escherichia coli antibody
Goat anti Escherichia coli antibody (OBT986)used to evaluate E. coli colonization in murine colonic mucosa by immunofluorescence.
Image caption:
Western diet selects a colitogenic microbiota favoring AIEC colonization.
(A) GF mice receiving a conventional or a HF/HS fecal transplantation were inoculated intragastrically with AIEC LF82. (A) Fecal counts of AIEC LF82 bacteria were performed (B) E. coli colonization of the colonic mucosa was compared between mice transplanted with a fecal pellet from conventional or HF/HS donor mice using confocal microscopy and quantification was performed using the ImageJ software. ****P <0.0001.
From: Agus A, Denizot J, Thévenot J, Martinez-Medina M, Massier S, Sauvanet P, Bernalier-Donadille A, Denis S, Hofman P, Bonnet R, Billard E, Barnich N.
Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 2016 Jan 8;6:19032.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT986) used to evaluate E. coli colonization in murine intestinal mucosa by immunofluorescence.
Image caption:
Western diet favors emergence of E. coli associated with ileal, cecal and colonic mucosa.
(A) E. coli bacteria were isolated from the mucosa by plating on Drigalski agar plates and the number of bacteria was determined by counting the CFU. (B) E. coli colonization of the ileal mucosa of CEABAC10 mice under a conventional or an HF/HS diet was visualized by confocal microscopy and quantification was performed by assessing global fluorescence intensities using the ImageJ software. ****P <0.0001.
From: Agus A, Denizot J, Thévenot J, Martinez-Medina M, Massier S, Sauvanet P, Bernalier-Donadille A, Denis S, Hofman P, Bonnet R, Billard E, Barnich N.
Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 2016 Jan 8;6:19032.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Immunostaining of the GPI-anchored mannosylated FimH receptor CD48 on the J774A.1 macrophage cell membrane. Deconvolved confocal fluorescence and interference reflection micrographs (IRM). Samples were stained for macrophage actin (white), CD48 (yellow) and E. coli (red). CD48 exclusively localized within the macrophage membrane including filopodia (see arrowheads and x-z and y-z cross sections). Filopodia (FP) and lamellipodia (LP) – bacteria contacts were suggested by IRM.
From: Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism
Möller J, Lühmann T, Chabria M, Hall H, Vogel V.
Scientific Reports 2013: 3, Article number: 2884
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Immunostaining of the GPI-anchored mannosylated FimH receptor CD48 on the J774A.1 macrophage cell membrane. Deconvolved confocal fluorescence and interference reflection micrographs (IRM). Samples were stained for macrophage actin (white), CD48 (yellow) and E. coli (red). CD48 exclusively localized within the macrophage membrane including filopodia (see arrowheads and x-z and y-z cross sections). Filopodia (FP) and lamellipodia (LP) – bacteria contacts were suggested by IRM.
From: Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism
Möller J, Lühmann T, Chabria M, Hall H, Vogel V.
Scientific Reports 2013: 3, Article number: 2884
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to visualize the presence of colonic mucosa associated bacteria in mice by immunofluorescence.
Image caption:
Immunofluorescence staining of E. coli associated to colonic mucosa. E. coli bacteria were stained in green, DNA was stained in blue. White arrows indicate E. coli bacteria.
From: Gimier E, Chervy M, Agus A, et al.
Methyl-donor supplementation prevents intestinal colonization by Adherent-Invasive E. coli in a mouse model of Crohn's disease.
Sci Rep. 2020;10(1):12922.
doi:10.1038/s41598-020-69472-3
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to detect E. coli. in mouse bladder by immunofluorescence
Image caption:
Impact of high glucose and E. coli infection on the cytoskeleton.
Representative sections from mouse bladders 24 h post infection, stained for Rhob and E. coli in non-diabetic, C57BL/6j and diabetic mice (n=5 each). Expression of Rhob detected in the superficial umbrella cells (Mann-Whitney two-tailed test, p=0.0075), relative number of IBCs in each mouse (n=6 each) (unpaired two-tailed test, p=0.0013). In vitro experiments were performed in duplicate or triplicate with at least 3 independent experiments and presented as mean + SEM, statistical outliers defined by Grubb’s test were excluded. For in vivo analysis individual values and median are shown, **p<0.01 and ****p<0.0001.
From: Mohanty S, Kamolvit W, Scheffschick A, Björklund A, Tovi J, Espinosa A, Brismar K, Nyström T, Schröder JM, Östenson CG, Aspenström P, Brauner H, Brauner A.
Diabetes downregulates the antimicrobial peptide psoriasin and increases E. coli burden in the urinary bladder. Nat Commun. 2022 Sep 20;13(1):4983.
doi: 10.1038/s41467-022-32636-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to detect E. coli. in mouse bladder by immunofluorescence
Image caption:
The impact of estradiol and high glucose on bacterial clearance.
Bacterial load in a urine and b urinary bladders of non-diabetic, C57BL/6j (n = 8 each) and diabetic, db/db (n = 6, 8 mice respectively) after 24 h E. coli infection (unpaired two-tailed t test, p = 0.0003, p ≤ 0.0001). c Representative mouse bladder sections stained for UPIIIa and E. coli in C57BL/6j (n = 8) and db/db mice (n = 7). L.p., lamina propria; Lu., lumen; Ep., epithelium. Bacterial load in d urine and e urinary bladders of C57BL/6j (n = 5 and 6) and db/db (n = 6 in each group) after 14 days E. coli infection (unpaired two-tailed t test, p = 0.0242).
From: Mohanty S, Kamolvit W, Scheffschick A, Björklund A, Tovi J, Espinosa A, Brismar K, Nyström T, Schröder JM, Östenson CG, Aspenström P, Brauner H, Brauner A.
Diabetes downregulates the antimicrobial peptide psoriasin and increases E. coli burden in the urinary bladder. Nat Commun. 2022 Sep 20;13(1):4983.
doi: 10.1038/s41467-022-32636-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to detect E. coli. in mouse bladder by immunofluorescence
Image caption:
The impact of high glucose on bacterial clearance. Total bacterial count was determined in (a) urine and (b) urinary bladders of non-diabetic, C57BL/6j (n=8,8) and diabetic, db/db (n=6,7) mice respectively after 7 days of E. coli infection (unpaired twotailed t test, p≤0,0001). Representative sections from mouse bladders infected for (c) 7 were stained for UPIIIa and E. coli in C57BL/6j and db/db mice (n =6 each). L.p., lamina propria; Lu., lumen; Ep., epithelium.
From: Mohanty S, Kamolvit W, Scheffschick A, Björklund A, Tovi J, Espinosa A, Brismar K, Nyström T, Schröder JM, Östenson CG, Aspenström P, Brauner H, Brauner A.
Diabetes downregulates the antimicrobial peptide psoriasin and increases E. coli burden in the urinary bladder. Nat Commun. 2022 Sep 20;13(1):4983.
doi: 10.1038/s41467-022-32636-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to detect E. coli. in mouse bladder by immunofluorescence
Image caption:
The impact of high glucose on bacterial clearance.
Representative sections from mouse bladders infected for (d) 14 days were stained for UPIIIa and E. coli in C57BL/6j and db/db mice (n =6 each). L.p., lamina propria; Lu., lumen; Ep., epithelium.
From: Mohanty S, Kamolvit W, Scheffschick A, Björklund A, Tovi J, Espinosa A, Brismar K, Nyström T, Schröder JM, Östenson CG, Aspenström P, Brauner H, Brauner A.
Diabetes downregulates the antimicrobial peptide psoriasin and increases E. coli burden in the urinary bladder. Nat Commun. 2022 Sep 20;13(1):4983.
doi: 10.1038/s41467-022-32636-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to label bacteria in mouse bradder sections by immunofluorescence.
Image caption:
HIF-1 activation decreased bacterial load in diabetic mice and uroepithelial cells. a Total number of bacteria in TERT-NHUC cells (n = 6, Wilcoxon matched-pairs signed-rank test) 24 h pretreated with vehicle or DMOG and urinary bladder from DMOG-treated diabetes, db/db (n = 8) and non-treated (n = 7) mice after b 24 h and c 7 days using CFU, colony-forming unit assay (unpaired t test). d Representative sections from mouse bladders from 7 days post-infection were stained for UPIIIa (marker of terminally differentiated umbrella cells, green) and E. coli (red), with DAPI to show nuclei (blue). L.p., lamina propria; Lu., lumen; Ep., epithelium. In vitro experiments were performed in either duplicate or triplicate. Data are shown as mean ± SEM. Results from mice are presented as median. Significance levels mentioned as *P < 0.05 and ***P < 0.001
From: Mohanty S, Kamolvit W, Zambrana S, Gonzales E, Tovi J, Brismar K, Östenson CG, Brauner A.
HIF-1 mediated activation of antimicrobial peptide LL-37 in type 2 diabetic patients.
J Mol Med (Berl). 2022 Jan;100(1):101-13.
doi: 10.1007/s00109-021-02134-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF ResetGoat anti Escherichia coli
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Escherichia coli
Antibody Quality and Performance Guarantee
| Goat anti Escherichia coli antibody recognizes many O and K antigenic serotypes of Escherichia coli. Goat anti Escherichia coli antibody is unabsorbed and cross reacts with related Enterobacteriaceae. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium azide
- Immunogen
- A mixture of Escherichia coli serotypes.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for Escherichia coli antibody
-
Möller, J. et al. (2013) Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism.
Sci Rep. 3: 2884. -
Raisch, J. et al. (2015) Intracellular colon cancer-associated Escherichia coli promote protumoral activities of human macrophages by inducing sustained COX-2 expression.
Lab Invest. 95 (3): 296-307. -
Lüthje P (2013) Estrogen supports urothelial defense mechanisms.
Sci Transl Med. 5: 190ra80. -
Agus A et al. (2016) Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 6: 19032. -
Gimier, E. et al. (2020) Methyl-donor supplementation prevents intestinal colonization by Adherent-Invasive E. coli in a mouse model of Crohn's disease.
Sci Rep. 10 (1): 12922. -
Mohanty, S. et al. (2022) Diabetes downregulates the antimicrobial peptide psoriasin and increases E. coli burden in the urinary bladder.
Nat Commun. 13 (1): 4983. -
Mohanty, S. et al. (2022) HIF-1 mediated activation of antimicrobial peptide LL-37 in type 2 diabetic patients.
J Mol Med (Berl). 100 (1): 101-13.
- RRID
- AB_618817
OBT0986
149795
158742
162580
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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