Escherichia coli antibody
Goat anti Escherichia coli antibody (OBT986)used to evaluate E. coli colonization in murine colonic mucosa by immunofluorescence.
Image caption:
Western diet selects a colitogenic microbiota favoring AIEC colonization.
(A) GF mice receiving a conventional or a HF/HS fecal transplantation were inoculated intragastrically with AIEC LF82. (A) Fecal counts of AIEC LF82 bacteria were performed (B) E. coli colonization of the colonic mucosa was compared between mice transplanted with a fecal pellet from conventional or HF/HS donor mice using confocal microscopy and quantification was performed using the ImageJ software. ****P <0.0001.
From: Agus A, Denizot J, Thévenot J, Martinez-Medina M, Massier S, Sauvanet P, Bernalier-Donadille A, Denis S, Hofman P, Bonnet R, Billard E, Barnich N.
Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 2016 Jan 8;6:19032.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT986) used to evaluate E. coli colonization in murine intestinal mucosa by immunofluorescence.
Image caption:
Western diet favors emergence of E. coli associated with ileal, cecal and colonic mucosa.
(A) E. coli bacteria were isolated from the mucosa by plating on Drigalski agar plates and the number of bacteria was determined by counting the CFU. (B) E. coli colonization of the ileal mucosa of CEABAC10 mice under a conventional or an HF/HS diet was visualized by confocal microscopy and quantification was performed by assessing global fluorescence intensities using the ImageJ software. ****P <0.0001.
From: Agus A, Denizot J, Thévenot J, Martinez-Medina M, Massier S, Sauvanet P, Bernalier-Donadille A, Denis S, Hofman P, Bonnet R, Billard E, Barnich N.
Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 2016 Jan 8;6:19032.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Immunostaining of the GPI-anchored mannosylated FimH receptor CD48 on the J774A.1 macrophage cell membrane. Deconvolved confocal fluorescence and interference reflection micrographs (IRM). Samples were stained for macrophage actin (white), CD48 (yellow) and E. coli (red). CD48 exclusively localized within the macrophage membrane including filopodia (see arrowheads and x-z and y-z cross sections). Filopodia (FP) and lamellipodia (LP) – bacteria contacts were suggested by IRM.
From: Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism
Möller J, Lühmann T, Chabria M, Hall H, Vogel V.
Scientific Reports 2013: 3, Article number: 2884
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Image caption:
Immunostaining of the GPI-anchored mannosylated FimH receptor CD48 on the J774A.1 macrophage cell membrane. Deconvolved confocal fluorescence and interference reflection micrographs (IRM). Samples were stained for macrophage actin (white), CD48 (yellow) and E. coli (red). CD48 exclusively localized within the macrophage membrane including filopodia (see arrowheads and x-z and y-z cross sections). Filopodia (FP) and lamellipodia (LP) – bacteria contacts were suggested by IRM.
From: Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism
Möller J, Lühmann T, Chabria M, Hall H, Vogel V.
Scientific Reports 2013: 3, Article number: 2884
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody (OBT0986) used to visualize the presence of colonic mucosa associated bacteria in mice by immunofluorescence.
Image caption:
Immunofluorescence staining of E. coli associated to colonic mucosa. E. coli bacteria were stained in green, DNA was stained in blue. White arrows indicate E. coli bacteria.
From: Gimier E, Chervy M, Agus A, et al.
Methyl-donor supplementation prevents intestinal colonization by Adherent-Invasive E. coli in a mouse model of Crohn's disease.
Sci Rep. 2020;10(1):12922.
doi:10.1038/s41598-020-69472-3
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Escherichia coli antibody recognizes many O and K antigenic serotypes of Escherichia coli. Goat anti Escherichia coli antibody is unabsorbed and cross reacts with related Enterobacteriaceae.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium azide
- Preservative Stabilisers
- 0.1% Sodium azide
- Immunogen
- A mixture of Escherichia coli serotypes.
- Approx. Protein Concentrations
- 4.0 mg/ml
- Approx. Protein Concentrations
- 4.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Escherichia coli antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for Escherichia coli antibody
-
Möller, J. et al. (2013) Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism.
Sci Rep. 3: 2884. -
Raisch, J. et al. (2015) Intracellular colon cancer-associated Escherichia coli promote protumoral activities of human macrophages by inducing sustained COX-2 expression.
Lab Invest. 95 (3): 296-307. -
Lüthje P (2013) Estrogen supports urothelial defense mechanisms.
Sci Transl Med. 5: 190ra80. -
Agus A et al. (2016) Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 6: 19032. -
Gimier, E. et al. (2020) Methyl-donor supplementation prevents intestinal colonization by Adherent-Invasive E. coli in a mouse model of Crohn's disease.
Sci Rep. 10 (1): 12922.
Fluorescent Spectraviewer
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