Diphtheria Toxin antibody
Goat anti Diphtheria toxin antibody (3710-0956) used to evaluate CRM197 expression in kinoid lysates by western blotting.
Image caption:
Characterization of mouse IL-4-K and IL-13-K. Generation of high molecular weight kinoids upon conjugation of IL-4 or IL-13 to CRM197 was confirmed using SDS-PAGE (a), western blots against IL-4 (b), IL-13 (c) or CRM197 (d).
From: Conde E, Bertrand R, Balbino B, Bonnefoy J, Stackowicz J, Caillot N, Colaone F, Hamdi S, Houmadi R, Loste A, Kamphuis JBJ, Huetz F, Guilleminault L, Gaudenzio N, Mougel A, Hardy D, Snouwaert JN, Koller BH, Serra V, Bruhns P, Grouard-Vogel G, Reber LL.
Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice.
Nat Commun. 2021 May 11;12(1):2574. doi: 10.1038/s41467-021-22834-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetGoat anti Diphtheria Toxin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Diphtheria Toxin
Antibody Quality and Performance Guarantee
| Goat anti diphtheria toxin antibody reacts with the diphtheria toxin/toxoid. Diphtheria toxin is an exotoxin secreted by various strains of Corynebacterium diphtheriae that is the cause of diphtheria. The active toxin is a single chain of 535 amino acids composed of 3 distinct structural/functional domains; and N-Terminal ADP-ribosyltransferase (catalytic domain); a region which facilitates the delivery of the catalytic domain across the cell membrane (transmembrane domain) and the eukaryotic cell receptor binding domain. Goat anti diphtheria toxin antibody also recognizes CRM197 (Conde et al.2021), a non-toxic mutant form of diphtheria toxin with an amino acid substitution at glycine-52 to glutamic acid (Giannini et al. 1984). |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Purified diphtheria toxoid
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for Diphtheria Toxin antibody
-
Conde, E. et al. (2021) Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice.
Nat Commun. 12 (1): 2574.
3710-0956
140915
162477
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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