Diphtheria Toxin antibody

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Goat anti Diphtheria Toxin:HRP

Goat anti Diphtheria Toxin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
3710-0970
Datasheet
C E WB
SDS
1 ml loader

3710-0956
Datasheet
E
SDS
1 ml loader

Goat anti diphtheria toxin antibody reacts with the diphtheria toxin/toxoid.

Diphtheria toxin is an exotoxin secreted by various strains of Corynebacterium diphtheriae that is the cause of diphtheria. The active toxin is a single chain of 535 amino acids composed of 3 distinct structural/functional domains; and N-Terminal ADP-ribosyltransferase (catalytic domain); a region which facilitates the delivery of the catalytic domain across the cell membrane (transmembrane domain) and the eukaryotic cell receptor binding domain.

Goat anti diphtheria toxin antibody also recognizes CRM197 (Conde et al.2021), a non-toxic mutant form of diphtheria toxin with an amino acid substitution at glycine-52 to glutamic acid (Giannini et al. 1984).

Product Details

Target Species
Bacterial
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.002%Thiomersal
1%Bovine Serum Albumin
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Purified diphtheria toxoid
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
4 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q6NK15
Regulatory
For research purposes only

Applications of Diphtheria Toxin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/200 1/1000
Immunohistology - Frozen 1/20 1/200
Western Blotting 1/20 1/200
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for Diphtheria Toxin antibody

  1. Conde, E. et al. (2021) Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice.
    Nat Commun. 12 (1): 2574.

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