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SWC8 Antibody | MIL3

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  • Mouse anti Pig SWC8
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1219C, Fdatasheet pdfdatasheet pdf2 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8.

      Clone MIL3 has been used in two colour flow cytometry with clone MIL2 to distinguish between monocytes and granulocytes (Haverson et al. 1994).
    • Intended Use
    • Target Species
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Porcine Lamina Propria Leucocytes
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/20
      Immunohistology - Frozen1/101/50

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 50ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional SWC8 Antibody Formats

    Formats Clone Applications Sizes available
    SWC8 Antibody : S/N MIL3 C, F 2 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      Goat anti Mouse IgM:FITCSTAR86F1 mgF
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry


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