PureBlu™ Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for flow cytometry, fluorescence microscopy and cell imaging applications.

Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format. 

PureBlu Nuclear Staining Dye One-Step Dilution

PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution — no weighing required.

PureBlu Nuclear Staining Dye One-Step Dilution

Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to 50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM); PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)). Note: the optimal concentration for different cell types and applications should be determined empirically.

Features and Benefits of PureBlu Nuclear Staining Dyes

  • High purity formulation (>95%) for reliable results in challenging experiments
  • Pre-aliquoted powder format eliminates time-consuming weighing steps
  • Convenient format allows you to generate working solution with only one dilution step after resuspension
  • Compatible with multicolor experiments

Applications and Uses of PureBlu Nuclear Staining Dyes

  • Nuclear staining
  • Microscopy and cell imaging
  • Multicolor experiments
  • Cell Cycle analysis
  • DAPI can be used for cell viability analysis in flow cytometry

Ordering Information

PureBlu™ Nuclear
Staining Dye
Maximum, nm
Maximum, nm
Optimal Excitation Laser, nm S3e Cell Sorter ZE5 Cell Analyzer ZOE Fluorescent Cell Imager
135-1303 DAPI* 359 461 355
135-1304 Hoechst 33342** 350 461 355  

* Includes 5x50 µg vials of DAPI powder.
** Includes 5x56 µg vials of Hoechst 33342 powder.

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