p38 Alpha MAPK Antibody

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Western blot analysis of (A) HeLa, (B) HL-60 and (C) NIH/3T3 whole cell lysates probed with Rabbit anti p38 alpha MAPK

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  • Rabbit anti p38 Alpha MAPK
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2410WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2410
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti p38 alpha MAPK antibody recognizes mitogen-activated protein kinase p38 alpha (p38-alpha MAPK), also known as MAP kinase 14 (MAPK14) and stress activated protein kinase 2a (SAPK2a). p38 alpha is a member of the p38 MAPK serine/threonine kinase protein family, which also includes p38-beta (MAPK11), p38-gamma (MAPK12) and p38-delta (MAPK13) (Cargnello and Roux 2011).

      p38 alpha MAPK becomes activated in response to a variety of stimuli, which include inflammatory cytokines and oxidative stress (Cargnello and Roux 2011). p38 alpha MAPK plays an essential role in mediating inflammation by regulating the expression of tumor necrosis factor alpha (TNF alpha) and interleukin 1 (IL-1) (Dhillon et al. 2007). p38 MAPK proteins have been implicated in cancer development and p38 alpha MAPK has been proposed to act as a tumor suppressor (Dhillon et al. 2007).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Antiserum Preparation
      Antiserum to p38 alpha MAPK was raised by repeated immunisation of rabbits with highly purified antigen
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.2% Gelatin
    • Immunogen
      Peptide mapping to the carboxy terminus of mouse p38 alpha MAPK and sharing 100% homology with the human sequence
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti p38 alpha MAPK recognizes a band of approximately 38 kDa when run under reducing conditions
    • Instructions For Use

    Additional p38 Alpha MAPK Antibody Formats

    Formats Applications Sizes available
    p38 Alpha MAPK Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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