IgM Antibody

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IgM Antibody gallery image 1

Figure A. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse IgM isotype control (MCA692) detected with goat anti mouse IgM PE (STAR86PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM (STAR86PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgM Antibody gallery image 2

Figure A. Purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM PE (STAR86PE) and mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM PE (STAR86PE) and mouse anti porcine CD3 FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • IgM Antibody thumbnail image 1
  • IgM Antibody thumbnail image 2
  • Goat anti Mouse IgM:HRP
  • Goat anti Mouse IgM
  • Goat anti Mouse IgM:RPE
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR86PEFdatasheet pdfdatasheet pdf0.5 ml
    STAR86PE
    STAR86PC, E, Pdatasheet pdfdatasheet pdf1 mg
    STAR86P
    STAR86Edatasheet pdfdatasheet pdf1 mg
    STAR86
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Mouse IgM antibody recognizes murine IgM and is cross-adsorbed to reduce reactivity with other mouse heavy or light chains.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
    • Preparation
    • Antiserum Preparation
      Antisera to mouse IgM were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      HRP Stabiliser (BUF052A)
      0.01% Thiomersal
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Mouse IgM mu heavy chain.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of reconstitution.
    • GO Terms
      transmembrane receptor activity
      humoral immune response mediated by circulating immunoglobulin
      positive regulation of B cell proliferation
      immunoglobulin complex, circulating
      external side of plasma membrane
      B cell receptor signaling pathway
      protein binding
      integral to membrane
      positive regulation of MAPKKK cascade
      positive regulation of endocytosis
      early endosome to late endosome transport
      antigen binding
      B cell receptor complex
      positive regulation of peptidyl-tyrosine phosphorylation
      perinuclear region of cytoplasm
      activation of MAPK activity
      antigen processing and presentation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1000
      Immunohistology - Frozen
      Immunohistology - Paraffin1/50
    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml as coating antibody
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgM Antibody Formats

    Formats Applications Sizes available
    IgM Antibody : HRP C, E, P 1 mg
    IgM Antibody : RPE F 0.5 ml
    IgM Antibody : Purified E 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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