IgG Antibody

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IgG Antibody gallery image 1

Detection of Mouse anti Strap-tag-immo (MCA2488 ) in a flow cytometric analysis of strep tagged Human anti Human CD127 (HCA144 ) in human peripheral blood lymphocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Equine CD11a/CD18 (MCA1081 ) in a flow cytometric analysis of equine peripheral blood lymphocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Human CD98 (MCA1105G ) in a flow cytometric analysis of human peripheral blood monocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Canine CD18 (MCA1780GA ) in a flow cytometric analysis of canine peripheral blood monocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Human CD69 (MCA2806GA ) in a flow cytometric analysis of in vitro PHA stimulated peripheral nlood mononuclear cells using Rabbit F(ab7#39;)2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Human CD16 (MCA5665 ) in a flow cytometric analysis of human peripheral blood lymphocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti human CD49c (MCA5694 ) in a flow cytometric analysis of A431 epidermoid carcinoma cells using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Human CD300a (MCA5747 ) in a flow cytometric analysis of human peripheral blood monocytes using Rabbit F(ab7#39;)2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Human Siglec-5/Siglec-14 (MCA5787GA ) in a flow cytometric analysis of human peripheral blood monocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Detection of Mouse anti Rabbit CD4 (MCA799GA ) in a flow cytometric analysis of rabbit peripheral blood lymohocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B)

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Published customer image:
FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody (STAR9B) used for the detection of ALX receptor on transfected HEK293 cells by flow cytometry.
Image caption:
Transfection of ALX receptor into HEK293 cells.
A) Generation of the HEK293 clones. pRc/CMV expression vector used to transfect ALX receptor into HEK293 cells to produce stable cell lines. Details of the full length protein sequence for the ALX receptor are also shown. B) Representative expression of FPR receptors by PCR visualised on a 1% agarose gel: CMV, empty vector transfected cells; ALX, annexin A1 receptor transfected cells; (+) human neutrophil cDNA positive control; (-) water replaced cDNA negative control. In all experiments results are representative of three separate experiments with similar results. C) Specific ALX receptor immunoreactive protein cell surface expression was determined using flow cytometry and compared to CMV stably transfected HEK293 cells.

From: Renshaw D, Montero-Melendez T, Dalli J, Kamal A, Brancaleone V, D'Acquisto F, et al. (2010)
Downstream Gene Activation of the Receptor ALX by the Agonist Annexin A1.
PLoS ONE 5(9): e12771.

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  • Rabbit F(ab')2 anti Mouse IgG:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR9BFdatasheet pdfdatasheet pdf1 mg
    STAR9B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG.

      Some cross reactivity with mouse IgM and IgA is expected, as is cross reactivity with rat IgG. Cross reactivity with human serum proteins has been minimised by solid phase adsorption.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 50ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : FITC F 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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