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FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody (STAR9B) used for the detection of ALX receptor on transfected HEK293 cells by flow cytometry.
Transfection of ALX receptor into HEK293 cells.
A) Generation of the HEK293 clones. pRc/CMV expression vector used to transfect ALX receptor into HEK293 cells to produce stable cell lines. Details of the full length protein sequence for the ALX receptor are also shown. B) Representative expression of FPR receptors by PCR visualised on a 1% agarose gel: CMV, empty vector transfected cells; ALX, annexin A1 receptor transfected cells; (+) human neutrophil cDNA positive control; (-) water replaced cDNA negative control. In all experiments results are representative of three separate experiments with similar results. C) Specific ALX receptor immunoreactive protein cell surface expression was determined using flow cytometry and compared to CMV stably transfected HEK293 cells.
From: Renshaw D, Montero-Melendez T, Dalli J, Kamal A, Brancaleone V, D'Acquisto F, et al. (2010)
Downstream Gene Activation of the Receptor ALX by the Agonist Annexin A1.
PLoS ONE 5(9): e12771.