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IgG antibody

Product Code Applications Pack Size List Price Your Price Qty
103001
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IB 1 mg loader
List Price Your Price
loader

Goat anti Mouse IgG antibody recognizes mouse IgG, recognising the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA.

Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity.

Target Species
Mouse
Product Form
Ig fraction - liquid
Preparation
Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
Borate buffered saline
Preservative Stabilisers
None Present
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Immunoblotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgG antibody

  1. Joimel, U. et al. (2010) Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
    BMC Cancer. 10: 375.
  2. Childs K et al. (2012) Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction.
    J Virol. 86 (7): 3411-21.
  3. Moalli, F. et al. (2015) Intravital and whole-organ imaging reveals capture of melanoma-derived antigen by lymph node subcapsular macrophages leading to widespread deposition on follicular dendritic cells.
    Front Immunol. 6: 114.
  4. Ramos-Sevillano, E. et al. (2016) PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
    PLoS Pathog. 12 (3): e1005500.
  5. Abbate, F. et al. (2016) Acid-sensing ion channel immunoreactivities in the cephalic neuromasts of adult zebrafish.
    Ann Anat. 207: 27-31.
  6. Shea, G.K. et al. (2020) Juxtacrine signalling via Notch and ErbB receptors in the switch to fate commitment of bone marrow-derived Schwann cells.
    Eur J Neurosci. 52 (5): 3306-21.
  7. Siqueira, R.F. & Fernandes, R.L. (2018) Cryopreservation of lymphocytes for immunological studies in horses
    Pesquisa Veterinária Brasileira. 38 (11): 2019-22.
  8. Miguelena Chamorro, B. et al. (2023) Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
    Immunohorizons. 7 (11): 788-805.
  9. View The Latest Product References

Immunofluorescence

RRID
AB_609693
UniProt
P01867
P01865
P01863
P01864
P01868
P01869
P03987
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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