IgG Antibody

//www.bio-rad-antibodies.com/mouse-igg-antibody-103001.html?RenderPageSpecific=1
IgG Antibody gallery image 1

Published customer image:
Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescence
Image caption:
Immunolabelling of Mf by the monocyte/Mf marker CD14 and the M1 Mf marker CD80. Mf were seeded at a density of 5.7 × 104 cells/cm 2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 µg/ml LPS and 5 ng/ml IFN? for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mf cultured alone and was not detectable on Mf in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.

From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C. Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate. BMC Cancer. 2010 Jul 17;10:375.

Enlarge
IgG Antibody gallery image 2

Published customer image:
biotin cinjugated Goat anti Mouse IgG used in conjunction with horseradish peroxidase conjugated streptavidin (710005) for the detection of anti tumor derived antigen IgG by ELISA.
Image caption:
Local melanoma tumors generate systemic circulating IgG levels. (A). Outline of local tumor injection experiment. B16.F10-tdTom+ cells were s.c. injected into C57BL/6 mice on day 0 and allowed to proliferate for 30 days. In some mice, B16.F10-tdTom lysate was additionally s.c. injected to test whether IgG production can be boosted. (B) Brightfield (top) and red fluorescent (bottom) stereomicroscopic images of tumor-injected and contralateral footpads on day 30. (C) IgG titers in control and tumor-bearing mice on day 30, with and without additional B16.F10-tdTom+ cell lysate injection for 15 days. Each dot represents the serum of one mouse. Data in (C) are pooled from two independent experiments and were analyzed by a Kruskal–Wallis test with Dunn’s multiple comparison test. **p < 0.01, ***p < 0.001.

From: F. Moalli, S. T. Proulx, R. Schwendener, M. Detmar, C. Schlapbach, and J. V. Stein, “Intravital and Whole-Organ Imaging Reveals Capture of Melanoma-Derived Antigen by Lymph Node Subcapsular Macrophages Leading to Widespread Deposition on Follicular Dendritic Cells,” Front. Immunol., vol. 6, Mar. 2015.

Enlarge
IgG Antibody gallery image 3

Published customer image:
Texas Red® conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.
Image caption:
Recognition of S. pneumoniae by PSGL-1 is a generic event. Co-localization of FAMS-SE-labeled S. pneumoniae clinical isolates of different serotypes with PSGL-1 in HL-60 cells differentiated to neutrophils. Cellular DNA was stained with Hoechst whereas PSGL-1 was visualized with mouse anti-human PSGL-1 antibody followed by a goat anti-mouse Texas-Red staining. (DIC, Differential interference contrast).

From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)
PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog 12(3): e1005500.

Enlarge
IgG Antibody gallery image 4

Published customer image:
Texas Red® conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.
Image caption:
Local co-localization of FAM-SE S. pneumoniae (green), Cellular DNA (blue) and PSGL-1 (red) is shown and quantified by plotting the fluorescence intensity and the distance (in μm).

From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)
PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.
PLoS Pathog 12(3): e1005500.

Enlarge
  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • Goat anti Mouse IgG:Biotin
  • Goat anti Mouse IgG:Alk. Phos.
  • Goat anti Mouse IgG:BGAL
  • Goat anti Mouse IgG:Texas Red®
  • Goat anti Mouse IgG
  • Goat anti Mouse IgG:TRITC
  • Goat anti Mouse IgG:RPE
  • Goat anti Mouse IgG:FITC
  • Goat anti Mouse IgG:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
9 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    103009F, IFdatasheet pdfdatasheet pdf0.5 mg
    103009
    103008E, IB, IFdatasheet pdfdatasheet pdf1 mg
    103008
    103007IFdatasheet pdfdatasheet pdf1 mg
    103007
    103001E, IBdatasheet pdfdatasheet pdf1 mg
    103001
    103003IFdatasheet pdfdatasheet pdf1 mg
    103003
    103002F, IFdatasheet pdfdatasheet pdf1 mg
    103002
    103004Edatasheet pdfdatasheet pdf1 ml
    103004
    103006Edatasheet pdfdatasheet pdf1 ml
    103006
    103005Edatasheet pdfdatasheet pdf1 ml
    103005
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Mouse IgG antibody recognizes mouse IgG, recognising the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA.

      Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purfied IgG conjugated to Beta Galactosidase - liquid
      Purified IgG conjugated to Texas Red® - liquid
      Ig fraction - liquid
      Ig Fraction conjugated to Rhodamine (TRITC) - liquid
      Purified Ig conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
      Purified IgG prepared by affinity chromatography
      Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
      Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
      Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
      Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
      Purified IgG prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
      Purified IgG prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose.
    • Antiserum Preparation
      Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1%Sodium Azide
      50%Glycerol
      0.1% Sodium Azide (NaN3)
      50% Glycerol
      0.1% Sodium Azide (NaN3)
      None Present
      0.1%Sodium Azide (NaN3)
      0.1% Sodium Azide (NaN3)
      Stabilizing agent
      0.1% Sodium Azide (NaN3)
      50%Glycerol
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      Ig concentration 1.0 mg/ml
      Ig concentration 1.0 mg/ml
      Ig concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
      Phosphate buffered saline
      Borate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      Please see label for expiry date.
      Please see label for expiry date.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
      antibody-dependent cellular cytotoxicity
      extracellular region
      integral to membrane
      antigen binding
      positive regulation of type I hypersensitivity
      phagocytosis, recognition
      phagocytosis, engulfment
      complement activation, classical pathway
      positive regulation of type IIa hypersensitivity
      humoral immune response mediated by circulating immunoglobulin
      positive regulation of phagocytosis
      immunoglobulin complex, circulating
      plasma membrane
      antibacterial humoral response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Texas Red® (TXRD) is a registered trademark on Molecular Probes, Inc.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5,0001/20,000
      Immunoblotting
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2001/500
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
      Immunoblotting
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/5000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Texas Red® IF 1 mg
    IgG Antibody : FITC F, IF 1 mg
    IgG Antibody : Bgal E 1 ml
    IgG Antibody : HRP E 1 ml
    IgG Antibody : RPE F, IF 0.5 mg
    IgG Antibody : Alk. Phos. E 1 ml
    IgG Antibody : Tritc IF 1 mg
    IgG Antibody : Purified E, IB 1 mg
    IgG Antibody : Biotin E, IB, IF 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Write your review

              You may also be interested in...