Collagen IV Antibody

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Rabbit anti Mouse collagen 4 antibody (2150-1470) used for detectiuon of collagen 4 in rat retina by immunofluorescence on cryostat sections.
Image caption:
Retinal gliosis and subretinal neovascularization in RCS rats. Double label immunostaining was performed using antibodies against vimentin (red) and collagen type IV (green) on frozen sections. (A) Non-dystrophic rat at 21 weeks (w) of age showed a normal distribution of retinal vessels confined to the inner retina and absence of glial cells in the subretinal space. (B, C) RCS rats at 6 w (B) and 9 w (C) of age showed activation of Muller cells but without subretinal neovascularization. Muller cell invasion into the subretinal space was more obvious at 9 w compared with 6 w of age (arrows in C). (D) Subretinal neovascularization (arrows) in RCS rats at 13 w of age. GCL = ganglion cell layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, SP = subretinal space, CC = choroidal capillaries. Scale bars: 50 μm.

From: Shen W, Chung SH, Irhimeh MR, Li S, Lee S-R, et al. (2014)
Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats.
PLoS ONE 9(8): e104759.

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Published customer image:Rabbit anti Mouse collagen 4 antibody (2150-1470) used for the detection of collogen 4 expression in Elk3 knockout mice by immunofluorescence.
Image caption:
Smooth muscle actin and CollagenIV levels are not altered in Elk3(-/-) knockout retinae. (A) IsolectinB4 (ILB4) (left) and smooth muscle actin (SMA) (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(-/-) knockout animals. (B) IsolectinB4 (left) and collagenIV (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(-/-) knockout animals. (C) Quantitation of collagenIV RNA levels in control and Elk3(-/-) knockout retinae (n = 5 independent experiments). (D) Representative pairs of control and Elk3(-/-) knockout retinal lysates tested for smooth muscle actin expression in Western Blot analysis. (E) Quantitation of control and Elk3(-/-) knockout retinal levels of SMA (n = 5 independent experiments). Scale bar in (A, B left and middle) 1000 μm, in (A, B right) 100 μm.

From: Weinl C, Wasylyk C, Garcia Garrido M, Sothilingam V, Beck SC, et al. (2014)
Elk3 Deficiency Causes Transient Impairment in Post-Natal Retinal Vascular Development and Formation of Tortuous Arteries in Adult Murine Retinae.
PLoS ONE 9(9): e107048.

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Published customer image:Rabbit anti Mouse collagen IV antibody (2150-1470) used for the evaluation of collagen IV expression in murine pupillary membranes by immunofluorescence on cryosections.
Image caption:
Vhltrp1-creKO mice demonstrate a Hif1a-dependent persistence of the pupillary membrane. (A-I) Autofluorescence scanning laser ophthalmoscope (AF-SLO) imaging (A,D,G), indocyanin-green angiography (ICGA; B,E,H) and collagen IV-stained cryosections (C,F,I) of Vhltrp1-creKO, Vhl;Hif1atrp1-creKO and control littermates at P21 show a persistent pupillary membrane in Vhltrp1-creKO mice that is absent in Vhl;Hif1atrp1-creKO and control mice. CB, ciliary body. Scale bar: 75 μm.

From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.

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Rabbit anti Mouse collagen IV antibody (2150-1470) used for the evaluation of collagen IV expression in retinal flat-mounts by immunofluorescence.
Image caption:
Vhltrp1-creKO mice show a persistence of the hyaloid vasculature and a disorganised retinal vasculature that is independent of Hif1a. (A,B,F,G,K,L) Indocyanin-green angiography (A,F,K) and corresponding collagen 4-stained retinal flatmounts (B,G,L) show a persistent hyaloid vasculature in Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice that is absent in control littermates at P21. Inset in K shows angiography focussed on the persistent hyaloid vasculature. Inset in L shows a light microscopy image of a retinal flat mount with haemorrhage. (C,H,M) Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice demonstrate a disorganised retinal vasculature with areas of intra-retinal vascular proliferation compared with age-matched controls (hyaloid vessels were removed). Outlined regions indicate the areas selected for IMARES imaging. (D,E,I,J,N,O) IMARES reconstruction reveals a disorganised retinal vasculature with abrogated vascular layering and retinal vessels growing into the outer retina in Vhltrp1-creKO and Vhl;Hif1atrp1-creKO mice. Scale bars: 1 mm for B; 500 μm for C,M; 700 μm for H; 50 μm for D,E,I,J,N,O.

From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.

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Rabbit anti Mouse collagen IV antibody (2150-1470) used to visualize collagen IV expression in murine retina by immunofluorecence.
Image caption:
Overexpression of ATX causes abnormal vessel morphology and vessel regression. (A and B) Magnification view of angiogenic front in retina from ATX cTg mice at P6. Control and ATX cTg retinas had similar filopodia protrusion. Scale bar, 50 µm. TM, tamoxifen. (C and D) ATX cTg retinas displayed vessel regression at vascular plexus. Control (wild type) and ATX cTg retinas labeled for CD31 (green) and collagen IV (red). Arrows highlight empty collagen IV sleeves, indicating vessel regression. Scale bar, 100 μm. (E and F) Vessel regression was evaluated quantitatively. Error bars indicate s.d. (control; n = 7, ATX cTg; n = 5). P-values were estimated by student’s t-test, ***P < 0.001. Data were pooled from three independent experiments.

From: Yukiura H, Kano K, Kise R, Inoue A, Aoki J (2015)
Autotaxin Overexpression Causes Embryonic Lethality and Vascular Defects.Citation: Yukiura H, Kano K, Kise R, Inoue A, Aoki J (2015) Autotaxin Overexpression Causes Embryonic Lethality and Vascular Defects.
PLoS ONE 10(5): e0126734.

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Rabbit anti Mouse collagen IV antibody (2150-1470) used for the detection of collagen IV in mouse retina by immunofluorescence.
Image caption:
Deletion of Vhl in the RPE results in disruption of the retinal vascular architecture and formation of chorioretinal anastomoses. (A-L) Representative collagen IV-stained cryosections of control (A-D), Vhltrp1-creKO mice (E-H) and Vhl;Hif1atrp1-creKO (I-L) and at different time points during development show a disorganised retinal vasculature and the formation of chorioretinal anastomoses in Vhl;Hif1atrp1-creKO and Vhltrp1-creKO mice (G,K). GCL, ganglion cell layer; NBL, neuroblast layer; Cho, choroid; INL, inner nuclear layer, ONL, outer nuclear layer. Scale bars: 25 μm.

From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW.
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
Development. 2012 Jul;139(13):2340-50.

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Collagen IV Antibody gallery image 7

Published customer image:
Rabbit anti Mouse collagen IV antibody (2150-1470) used for the detection of collagen IV in Murine choroidal flatmounts by immunofluorescence on paraformaldehyde fixed sections.
Image caption:
Choroidal neovascularisation in CCL2-/-CX3CR1GFP/GFP DKO mice. (A) CNV was induced in adult (8~12-week) WT and CCL2-/-CX3CR1GFP/GFP mice. RPE/choroidal flatmounts were collected at day 10 and stained for isolectin B4 (green) and collagen IV (red) and examined by confocal microscopy. (B) CNV size in WT and CCL2-/-CX3CR1GFP/GFP mice at day 10. N?=?6. (C, D) VEGF-A mRNA expression in RPE cells (C) and BM-DMs (D) from WT and CCL2-/-CX3CR1GFP/GFP mice. N?=?5.

From: Chen M, Hombrebueno JR, Luo C, Penalva R, Zhao J, Colhoun L, et al. (2013)
Age- and Light-Dependent Development of Localised Retinal Atrophy in CCL2-/-CX3CR1GFP/GFP Mice.
PLoS ONE 8(4): e61381.

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Rabbit anti Murine cytokeratin 4 antibody (2150-1470) used for the identification of collagen IV expressing vasculature in mouse brains by immunofluorescence on vibratome sections.
Image caption:
Immunohistochemistry analysis of pericytes in Ang-2 GOF mice. a Pericytes (desmin+) were decreased in GOF mice utilizing 10 μm cryosections for analysis (n = 8, scale bars 25μm). b 50 μm vibratome sections of Ang-2 GOF and WT mice revealed decrease in pericyte area/vessel area [%] (n = 3, scale bars 10 μm) indicating decreased pericyte coverage of the vessels in GOF mice

From: Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, Sommer K, Di Tacchio M, Vutukuri R, Beck H, Mittelbronn M, Foerch C, Pfeilschifter W, Liebner S, Peters KG, Plate KH, Reiss Y.
Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling.
Acta Neuropathol. 2016 May;131(5):753-73.

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Rabbit anti Human collagen IV antibody (2150-1470) showing increased renal collagen IV deposition in aged PpargΔ/Δ mice over controls by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry and gene expression of fibrosis markers in kidney of ageing PpargΔ/Δ mice, and plasmatic levels of anti-β2-glycoprotein 1 antibodies.
52 weeks old control (CTL) and PparΔ/ΔΔ/Δ) animals were analyzed for the following parameters: (A) Top panels: Kidney Immunohistochemistry for collagen IV (Col-IV). Positive staining is in brown. Scale bar represents 100μm.

From: Toffoli B, Gilardi F, Winkler C, Soderberg M, Kowalczuk L, Arsenijevic Y, et al. (2017)
Nephropathy in Pparg-null mice highlights PPARγ systemic activities in metabolism and in the immune system.
PLoS ONE 12(2): e0171474.

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  • Rabbit anti Mouse Collagen IV
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-1470E, IF, Pdatasheet pdfdatasheet pdf0.1 ml
    2150-1470
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).

      Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).

      Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).

      The following cross reactivities have been observed:

      Mouse type IV100%
      Mouse types I, II & III<0.1%
      Human types IV & V<0.1%
      Mouse fibronectin & laminin<0.1%
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/400
      Immunofluorescence1/401/160
      Immunohistology - Paraffin1/2501/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen IV Antibody Formats

    Formats Applications Sizes available
    Collagen IV Antibody : Purified E, IF, P 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
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          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Immunohistology - Paraffin

              References

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