Rat anti Mouse CD4:FITC/CD8:RPE dual colour reagent was developed for the simultaneous recognition of mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognises murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognises a non-polymorphic epitope on the murine CD8 alpha chain.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
1. Darby, C.R. et al. (1992) Evidence that long-term cardiac allograft survival induced by anti-CD4 monoclonal antibody does not require depletion of CD4+ T cells.Transplantation. 54 (3): 483-90.
2. Lis, M. et al. (2013) Modulatory effects of bestatin on T and B lymphocyte subsets and the concentration of cytokines released by Th1/Th2 lymphocytes in cyclophosphamide-treated miceCentr Eur J Immunol 38: 42-53
4. Suszko A & Obmi&324;ska-Mrukowicz B (2013) Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate.J Ethnopharmacol. 145 (1): 109-17.
5. Aravind, S. et al. (2015) Protective effects of recombinant glycoprotein D based prime boost approach against duck enteritis virus in mice model.Microb Pathog. 88: 78-86.
6. Zimecki, M. et al. (2015) Immune function in cyclophosphamide-treated mice is restored by the T-cell-tropic isoxazole derivative R-13.J Immunotoxicol. 12 (4): 322-9.