Tubulin Alpha antibody | YOL1/34






























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WB IF ResetRat anti Tubulin Alpha
- Product Type
- Monoclonal Antibody
- Clone
- YOL1/34
- Isotype
- IgG2a
- Specificity
- Tubulin Alpha
Rat anti Tubulin alpha antibody, clone YOL1/34 recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2. Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin. |
- Target Species
- Yeast
- Species Cross-Reactivity
-
Target Species Cross Reactivity Birds Expected from Sequence Mammals Expected from Sequence Drosophila Fungal Expected from Sequence Human Arabidopsis thaliana Saccharomyces Platyzoma Ashbya Mouse Naegleria Asplenium nidus Seagrass (Cymodocea nodosa) - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Yeast tubulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fuzed with cells of the rat YB2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
10ug/ml as detecting antibody | |
Immunofluorescence | ![]() |
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Immunohistology - Frozen | ![]() |
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Radioimmunoassays | ![]() |
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Western Blotting | ![]() |
Source Reference
-
Kilmartin, J.V. et al. (1982) Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
J Cell Biol. 93 (3): 576-82.
References for Tubulin Alpha antibody
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Petronczki, M. et al. (2004) Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II.
J Cell Sci. 117: 3547-59. -
Chatzimeletiou, K. et al. (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy.
Hum Reprod. 20: 672-82. -
White, J. et al. (2005) Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation.
Mol Biol Cell. 16 (4): 2018-27. -
Yu, H.G. et al. (2007) The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
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D'Ambrosio, C. et al. (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.
Genes Dev. 22: 2215-27 -
Sullivan, M. et al. (2008) Cyclin-specific control of ribosomal DNA segregation.
Mol Cell Biol. 28: 5328-36.
View The Latest Product References
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Raspelli, E. et al. (2011) Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
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Goto, G.H. et al. (2011) Bub1-mediated adaptation of the spindle checkpoint.
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Rossio, V. and Yoshida, S. (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.
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Buerstenbinder, K. et al. (2013) Arabidopsis Calmodulin-binding IQD1 Localizes to Microtubules and Interacts with Kinesin Light Chain-Related Protein-1.
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Schweizer, N. et al. (2015) An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.
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Okamoto, M. et al. (2016) Fyn Accelerates M Phase Progression by Promoting the Assembly of Mitotic Spindle Microtubules.
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- RRID
- AB_325005
MCA78G


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