Tubulin Alpha antibody | YOL1/34

Rat anti Tubulin Alpha

Product Type
Monoclonal Antibody
Tubulin Alpha

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF R WB 0.5 mg loader
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Rat anti Tubulin alpha antibody, clone YOL1/34 recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2.

Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Birds Expected from Sequence
Mammals Expected from Sequence
Fungal Expected from Sequence
Arabidopsis thaliana
Asplenium nidus
Seagrass (Cymodocea nodosa)
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yeast tubulin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized LOU rats were fuzed with cells of the rat YB2/0 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 10ug/ml as detecting antibody
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Tubulin Alpha antibody

  1. Goto, G.H. et al. (2011) Bub1-mediated adaptation of the spindle checkpoint.
    PLoS Genet. 7: e1001282.
  2. Fonseca, A.V. et al. (2010) Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network.
    J Biol Chem. 285: 31661-71.
  3. Lang, C. et al. (2010) Mobility, microtubule nucleation and structure of microtubule-organizing centers in multinucleated hyphae of Ashbya gossypii.
    Mol Biol Cell. 21: 18-28.
  4. Petronczki, M. et al. (2004) Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II.
    J Cell Sci. 117: 3547-59.
  5. Rossio, V. et al. (2010) The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase
    J Cell Biol. 191: 981-97.
  6. Takeo, S. et al. (2010) Calcineurin and its regulation by Sra/RCAN is required for completion of meiosis in Drosophila.
    Dev Biol. 344: 957-67.
  7. Trieselmann, N. et al. (2003) Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation.
    J Cell Sci. 116: 4791-8.
  8. Whelan, F. et al. (2010) Amino acid substitutions in the aryl hydrocarbon receptor ligand binding domain reveal YH439 as an atypical AhR activator.
    Mol Pharmacol. 77: 1037-46.
  9. View The Latest Product References
  10. Hao, N. et al. (2013) Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation.
    Nucleic Acids Res. 41 (11): 5626-38.
  11. Kilmartin, J.V. et al. (1982) Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
    J Cell Biol. 93 (3): 576-82.
  12. Waples, W.G. et al. (2009) Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit.
    Mol Biol Cell. 20 (1): 245-55.
  13. White, J. et al. (2005) Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation.
    Mol Biol Cell. 16 (4): 2018-27.
  14. Lang, C. et al. (2010) Structural mutants of the spindle pole body cause distinct alteration of cytoplasmic microtubules and nuclear dynamics in multinucleated hyphae.
    Mol Biol Cell. 21 (5): 753-66.
  15. Hao, N. et al. (2011) Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR.
    Nucleic Acids Res. 39 (9): 3695-709.
  16. D'Ambrosio, C. et al. (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.
    Genes Dev. 22: 2215-27
  17. Hartl, T.A. et al. (2008) Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis.
    PLoS Genet. 4: e1000228.
  18. Mirchenko, L. and Uhlmann, F. (2010) Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset.
    Curr Biol. 20: 1396-401.
  19. Sullivan, M. et al. (2001) Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19.
    Nat Cell Biol. 3: 771-7.
  20. Sullivan, M. et al. (2008) Cyclin-specific control of ribosomal DNA segregation.
    Mol Cell Biol. 28: 5328-36.
  21. Yu, H.G. et al. (2007) The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
    J Cell Biol. 176: 911-8.
  22. Rossio, V. and Yoshida, S. (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.
    J Cell Biol. 193: 445-54.
  23. Grava, S. et al. (2011) Clustering of Nuclei in Multinucleated Hyphae Is Prevented by Dynein-Driven Bidirectional Nuclear Movements and Microtubule Growth Control in Ashbya gossypii.
    Eukaryot Cell. 10: 902-15.
  24. Raspelli, E. et al. (2011) Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
    Mol Biol Cell. 22: 2185-97.
  25. Finlayson, M.R. et al. (2011) Regulation of exit from mitosis in multinucleate Ashbya gossypii cells relies on a minimal network of genes.
    Mol Biol Cell. 22 (17): 3081-93.
  26. Chatzimeletiou, K. et al. (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy.
    Hum Reprod. 20: 672-82.
  27. Chatzimeletiou, K. et al. (2012) Cytoskeletal analysis of human blastocysts by confocal laser scanning microscopy following vitrification.
    Hum Reprod. 27: 106-13.
  28. Keeling, J.W. and Miller, R.K. (2011) Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast.
    Methods Mol Biol. 782: 231-44.
  29. Elhanany-Tamir, H. et al. (2012) Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules.
    J Cell Biol. 198 (5): 833-46.
  30. Walsh, C.J. (2012) The structure of the mitotic spindle and nucleolus during mitosis in the amebo-flagellate Naegleria.
    PLoS One. 7: e34763.
  31. Adamakis, I.D. et al. (2013) Effects of bisphenol A on the microtubule arrays in root meristematic cells of Pisum sativum L.
    Mutat Res. 750 (1-2): 111-20.
  32. Buerstenbinder, K. et al. (2013) Arabidopsis Calmodulin-binding IQD1 Localizes to Microtubules and Interacts with Kinesin Light Chain-Related Protein-1.
    J Biol Chem. 288: 1871-82.
  33. Panteris, E. et al. (2013) The distribution of TPX2 in dividing leaf cells of the fern Asplenium nidus.
    Plant Biol (Stuttg). 15: 203-9.
  34. Malea, P. et al. (2013) Microtubule integrity and cell viability under metal (Cu, Ni and Cr) stress in the seagrass Cymodocea nodosa.
    Chemosphere. pii: S0045-6535(13)00820-5.
  35. Eleftheriou, E.P. et al. (2016) Hexavalent chromium-induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of α-tubulin.
    Protoplasma. 253 (2): 531-42.
  36. Wang, S. et al. (2015) Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1.
    J Cell Biol. 209 (4): 529-38.
  37. Okamoto, M. et al. (2016) Fyn Accelerates M Phase Progression by Promoting the Assembly of Mitotic Spindle Microtubules.
    J Cell Biochem. 117 (4): 894-903.
  38. Eleftheriou, E.P. et al. (2015) Aberration of mitosis by hexavalent chromium in some Fabaceae members is mediated by species-specific microtubule disruption.
    Environ Sci Pollut Res Int. 22 (10): 7590-9.
  39. Sullivan, A.E. et al. (2016) MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors.
    FEBS J. 283 (18): 3488-502.
  40. Schweizer, N. et al. (2015) An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.
    J Cell Biol. 210 (5): 695-704.
  41. Livanos P et al. (2016) Deliberate ROS production and auxin synergistically trigger the asymmetrical division generating the subsidiary cells in Zea mays stomatal complexes.
    Protoplasma. 253 (4): 1081-99.
  42. Xie, J.L. et al. (2017) Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
    mSphere. 2 (2): Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
  43. Bacon, T. et al. (2015) Histone deacetylase 3 indirectly modulates tubulin acetylation.
    Biochem J. 472 (3): 367-77.
  44. Kowanda, M. et al. (2016) Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.
    Biol Open. 5 (8): 1040-51.
  45. Diao, L.T. et al. (2017) Delineation of the role of chromatin assembly and the Rtt101Mms1 E3 ubiquitin ligase in DNA damage checkpoint recovery in budding yeast.
    PLoS One. 12 (7): e0180556.
  46. Játiva, S. et al. (2019) Cdc14 activation requires coordinated Cdk1-dependent phosphorylation of Net1 and PP2A-Cdc55 at anaphase onset.
    Cell Mol Life Sci. 76 (18): 3601-20.
  47. Jühlen, R. et al. (2020) Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts.
    Sci Rep. 10 (1): 19301.
  48. Yam, C.Q.X. et al. (2020) Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.
    Nucleic Acids Res. 48 (11): 6092-107.
  49. Avilés-Pagán, E.E. et al. (2020) Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.
    G3 (Bethesda). 10 (9): 2989-2998.
  50. Morii, M. et al. (2021) Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
    Sci Rep. 11 (1): 2616.
  51. Scheffler, K. et al. (2021) Two mechanisms drive pronuclear migration in mouse zygotes.
    Nat Commun. 12 (1): 841.
  52. ElInati, E. et al. (2020) The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes.
    Nat Commun. 11 (1): 2598.
  53. Coronas-Serna, J.M. et al. (2020) The TIR-domain containing effectors BtpA and BtpB from Brucella abortus impact NAD metabolism.
    PLoS Pathog. 16 (4): e1007979.
  54. Stojic, L. et al. (2020) A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.
    Nat Commun. 11 (1): 1851.
  55. Giannoutsou, E. et al. (2019) De-Esterified Homogalacturonan Enrichment of the Cell Wall Region Adjoining the Preprophase Cortical Cytoplasmic Zone in Some Protodermal Cell Types of Three Land Plants.
    Int J Mol Sci. 21(1):81.
  56. Cavazza, T. et al. (2021) Parental genome unification is highly error-prone in mammalian embryos.
    Cell. Apr 30 [Epub ahead of print].
  57. Panteris, E. et al. (2018) Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin.
    J Biol Res (Thessalon). 25: 12.
  58. Giourieva, V. & Panteris, E. (2021) Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana..
    J Biol Res (Thessalon). 28 (1): 13.
  59. Malea, P. et al. (2021) Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea..
    Ecological Indicators. 122: 107224.


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