Tubulin Alpha antibody | YOL1/34
Rat anti Tubulin Alpha
- Product Type
- Monoclonal Antibody
- Clone
- YOL1/34
- Isotype
- IgG2a
- Specificity
- Tubulin Alpha
Rat anti tubulin alpha antibody, clone YOL1/34 recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2. |
- Target Species
- Yeast
- Species Cross-Reactivity
-
Target Species Cross Reactivity Birds Expected from Sequence Mammals Expected from Sequence Drosophila Fungal Expected from Sequence Human Arabidopsis thaliana Saccharomyces Platyzoma Ashbya Mouse Naegleria Asplenium nidus Seagrass (Cymodocea nodosa) Rye (Secale cereale L.) - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Yeast tubulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fused with cells of the rat YB2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 10ug/ml as detecting antibody | ||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Radioimmunoassays | |||
Western Blotting |
Source Reference
-
Kilmartin, J.V. et al. (1982) Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
J Cell Biol. 93 (3): 576-82.
References for Tubulin Alpha antibody
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Sullivan, M. et al. (2001) Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19.
Nat Cell Biol. 3: 771-7. -
Trieselmann, N. et al. (2003) Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation.
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Petronczki, M. et al. (2004) Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II.
J Cell Sci. 117: 3547-59. -
Chatzimeletiou, K. et al. (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy.
Hum Reprod. 20: 672-82. -
White, J. et al. (2005) Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation.
Mol Biol Cell. 16 (4): 2018-27. -
Yu, H.G. et al. (2007) The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
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D'Ambrosio, C. et al. (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.
Genes Dev. 22: 2215-27 -
Sullivan, M. et al. (2008) Cyclin-specific control of ribosomal DNA segregation.
Mol Cell Biol. 28: 5328-36.
View The Latest Product References
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Fonseca, A.V. et al. (2010) Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network.
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Raspelli, E. et al. (2011) Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
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Goto, G.H. et al. (2011) Bub1-mediated adaptation of the spindle checkpoint.
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Rossio, V. and Yoshida, S. (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.
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Panteris, E. et al. (2013) The distribution of TPX2 in dividing leaf cells of the fern Asplenium nidus.
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Buerstenbinder, K. et al. (2013) Arabidopsis Calmodulin-binding IQD1 Localizes to Microtubules and Interacts with Kinesin Light Chain-Related Protein-1.
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Adamakis, I.D. et al. (2013) Effects of bisphenol A on the microtubule arrays in root meristematic cells of Pisum sativum L.
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Okamoto, M. et al. (2016) Fyn Accelerates M Phase Progression by Promoting the Assembly of Mitotic Spindle Microtubules.
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- RRID
- AB_325005
MCA78G
151452 163138 169361If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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