Tubulin Alpha antibody | YOL1/34
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used as a loading control for the detection of tubulin-α in Candida albicans lysates by western blotting.
Image caption:
Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37°C, YPD plus 200 mM hydroxyurea (HU) at 30°C, or YPD plus 0.5 μg/ml staurosporine at 30°C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm.
From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE.
Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used for the illustration of mitotic spindles in wild type and Bsg25D mutant Drosophila embryos by immunofluorescence.
Image caption:
Detail from: Bsg25D mutant embryos have a reduced hatching rate and exhibit mitotic defects.
Anti-α-tubulin, anti-γ-tubulin immunostaining and DAPI staining of a wild-type embryo showing mitotic spindles. (G) Example of embryo graded as having severe mitotic defects. Two images of the same embryo showing two tripolar mitotic divisions (arrows) with γ-tubulin attached to mitotic spindle. In all images scale bar=50μm.
From: Kowanda M, Bergalet J, Wieczorek M, Brouhard G, Lécuyer É, Lasko P.
Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.
Biol Open. 2016 Aug 15;5(8):1040-51.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Cap-H2 mutants are defective in anaphase II segregation. Metaphase II and anaphase II morphologies were compared between wild-type and Cap-H2Z3-0019/Cap-H2TH1 mutant males. Testes were stained with DAPI and an anti-tubulin antibody to visualize DNA (white) and microtubules (green), respectively (scale bar in 6A indicates 10 μm). (A) Wild-type metaphase/anaphase II cyst. Metaphase II cells are those where each bivalent has congressed to the metaphase plate and appear as a cluster of DAPI staining material. Anaphase II are those cells with two DAPI staining white clusters, indicating homologous chromosome segregation. (B) Metaphase II and anaphase II figures from a Cap-H2 strong mutant. Arrow indicates an anaphase II bridge. The arrowhead highlights an anaphase II bridge or lagging chromosome.
From: Hartl TA, Sweeney SJ, Knepler PJ, Bosco G (2008)
Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis.
PLoS Genet 4(10): e1000228.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
T566 phosphorylation affects Bub1 stability. (A) BUB1-T566A mutant cells do not show substantial delay in recovering from nocodazole arrest. Wild-type (WT) and BUB1-T566A mutant cells were incubated with nocodazole (15 μg/mL) at 30°C for 90 min and then released from nocodazole arrest; at the indicated times, samples were taken to measure rebudded cells. (B) BUB1-T566A mutant cells show no significant sensitivity to nocodazole in a survival assay. Wild-type (WT), bub1Δ, and BUB1-T566A cells were incubated with nocodazole (15 μg/mL); at the indicated times, cells were washed out and approximately 200 cells were plated on a YPD plate. Cell viability was calculated by dividing the number of colonies formed at the 2.5 and 5 h time points by that formed in the absence of nocodazole (0 h) at 30°C. (C) Phosphorylation of T566 is important for degradation of Bub1 during anaphase but not during G1. cdc15-2 cells with HA-tagged Bub1 or Bub1-T566A expressed by the GAL1 promoter (GAL-BUB1 and GAL-BUB1-T566A) were arrested in anaphase (cdc15-2) or in G1 (alpha-factor) and then transferred to medium containing galactose for 2 h to induce Bub1 expression. Glucose was added to shut-off Bub1 expression; samples were taken at the indicated times for Western blot analyses with antibody to the HA epitope. Tubulin was used as a loading control. (D) The Bub1-T566A protein is more stable than wild-type in the presence of nocodazole. Wild-type and BUB1-T566A mutant cells (Bub1 and Bub1-T566A are tagged with myc) were incubated in the presence of nocodazole (10 µg/mL) at 30°C; at the indicated times, samples were taken for Western blot analyses with antibody to the myc epitope. Tubulin was used as a loading control.
From: Goto GH, Mishra A, Abdulle R, Slaughter CA, Kitagawa K (2011)
Bub1-Mediated Adaptation of the Spindle Checkpoint.
PLoS Genet 7(1): e1001282.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Stages of Mitosis in Naegleria revealed by staining for α-tubulin, BN46/51 and DAPI. Images were obtained by conventional epifluorescence microscopy. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. Bar = 10 μm. Red, BN46/51; Green, α-tubulin; Blue, DAPI.
Walsh CJ (2012)
The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria.
PLoS ONE 7(4): e34763.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Confocal Microscopy of Mitotic Cells Stained for α-Tubulin and the Nucleolar Protein BN46/51. Five examples of progressively later stages of each mitotic stage are presented. Each image is a Z-axis projection of 0.25 μm optical sections through an individual cell. The cell periphery is outlined in white. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. F. Selected nuclei of, from left to right, Prophase, Metaphase, Anaphase, and Telophase. Bar = 10 μm. Red, BN 46/51; Green, α-tubulin.
From: Walsh CJ (2012)
The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria.
PLoS ONE 7(4): e34763.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti tubulin-α antibody, clone YOL1/34 (MCA78G) used as loading control for transfected yeast cell lysates by western blotting
Image caption:
Protein expression and solubility. Analysis of the expression and the soluble/insoluble partition of Aβwt-GFP and Aβm-GFP. The total, soluble and insoluble fractions were separated after induction. Tubulin alpha (Tubα) was employed to normalise the Aβ-GFP bands.
From: Sanchez de Groot N, Gomes RA, Villar-Pique A, Babu MM, Coelho AV,
Ventura S.
Proteome response at the edge of protein aggregation.
Open Biol. 2015 Feb;5(2). pii: 140221.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Analysis of the Co-localization of Tubulin and the Nucleolar Protein BN46/51 During Prophase. Single 0.25 μm optical sections at 1.0 μm intervals are presented for two different cells. Columns A and D; raw data. Columns B and E; tubulin pixels are only displayed if the corresponding nucleolar protein pixel equaled or exceeded 25% of the maximum possible intensity. Columns C and F; tubulin pixels are only displayed if the corresponding nucleolar protein pixel equaled or exceeded 50% of maximum possible intensity. Numerical values are the summed fraction of tubulin intensity remaining in a given section after the subtraction. Red, BN 46/51; Green, α-tubulin. Bar = 10 μm.
From: Walsh CJ (2012)
The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria.
PLoS ONE 7(4): e34763.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti tubulin-α antibody, clone YOL1/34 (MCA78G) used for the detection of tubulin-α in Drosophila by immunofluorescence.
Image caption:
Chromosomes remain associated into anaphase I of Cap-H2 mutants. Metaphase I and anaphase I morphologies were compared between wild-type and Cap-H2Z3-0019/Cap-H2TH1 mutant males. Testes were stained with DAPI and an anti-tubulin antibody to visualize DNA (white) and microtubules (green), respectively (scale bar in 3A indicates 10 μm and 5 μm in 3G). (A) Metaphase I in the wild-type. Each bivalent has congressed to the metaphase plate and appears as a cluster of DAPI stained material. (B) Anaphase I in the wild-type (DAPI only). Homologous chromosomes have segregated to daughter cells. (C) Anaphase I in the wild-type (DAPI and Tubulin merge). (D) Metaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male appears wild-type. (E) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male (DAPI only). Chromatin bridges can be seen in three different segregation events. (F) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male (DAPI and Tubulin merge).
From: Hartl TA, Sweeney SJ, Knepler PJ, Bosco G (2008)
Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis.
PLoS Genet 4(10): e1000228.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti tubulin-α antibody, clone YOL1/34 (MCA78G) used for the detection of tubulin-α in HeLa cells by immunofluorescence.
Image caption:
Role of the phosphorylation of ATF7 in G2/M progression. (A) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were synchronized using DTB and released into thymidine-free medium containing 1 µg/ml Dox for 12 h. Whole cell lysates were analyzed by WB. Full-length blots are presented in S12A Fig. (B) HeLa S3/TR/ATF7-wt (cl.2) (upper panels) or HeLa S3/TR/ATF7-TA (cl.1) cells (lower panels) were synchronized using single-thymidine block and released into thymidine-free medium containing 1 µg/ml Dox for 11 h. Cells were triply stained with anti-ATF7[SAB2500131] and anti-α-tubulin antibodies and PI (for DNA). Scale bars, 20 µm. (C, D) Cells were stained with anti-histone H3pS10 antibody (for M phase) and PI for analyzing cell-cycle progression by flow cytometry. (C) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were synchronized using DTB and released into thymidine-free medium containing 1 µg/ml Dox for 10.5~12.5 h. Two-dimensional histograms (DNA vs histone H3pS10) are presented together with DNA histograms, and the percentages of cells in G1 and M phases were measured. Cells in M and and G1 phases were quantitated from the results of S4 Fig. Values are means ± SD (three independent clones). Asterisks indicate the significant difference (*P<0.05; NS, not significant), as calculated by Student’s t-test. (D) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were cultured in the presence of 9 µM RO-3306 for 10 h and treated with 1 µg/ml Dox for the last 5 h. The cells that were arrested at G2 phase were released into RO-3306-free medium containing 1 µg/ml Dox and incubated for 0, 10, and 20 min. Two-dimensional histograms (DNA vs histone H3pS10) are presented together with DNA histograms, and M-phase cells were quantitated. Values are means ± SD, n = 3 independent experiments. An asterisk indicates the significant difference (*P<0.05), as calculated by Student’s t-test.
From: Hasegawa H, Ishibashi K, Kubota S, Yamaguchi C, Yuki R, et al. (2014)
Cdk1-Mediated Phosphorylation of Human ATF7 at Thr-51 and Thr-53 Promotes Cell-Cycle Progression into M Phase.
PLoS ONE 9(12): e116048.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used for the evaluation of alpha tubulin expression in human fibroblast lysates by western blotting.
Image caption:
Ciliogenesis is impaired in FADS fibroblasts. Lysates from (e) serum-starved and (g) growing fibroblasts were subjected to Western blot analysis using antibodies against ac-tubulin, detyrosinated (detyr) α-tubulin, α-tubulin, and β-tubulin. CRM1 was used as loading control.
From: Jühlen R, Martinelli V, Vinci C, Breckpot J, Fahrenkrog B.
Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts.
Sci Rep. 2020 Nov 9;10(1):19301.
doi: 10.1038/s41598-020-76192-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin-α antibody, clone YOL1/34 (MCA78G) used to demonstrate the mitotic spindle in dividing yeast cells by immunofluorescence.
Image caption:
Dun1 deficient cells are unable to arrest in G2 in response to DNA damage.
Photomicrographs of cells at 6 h. Cell morphology, the state of mitotic spindle and the nucleus in both DUN1 and dun1Δ are shown. Individual cells are outlined (scale bar: 5 μm).
From: Yam CQX, Chia DB, Shi I, Lim HH, Surana U.
Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.
Nucleic Acids Res. 2020 Jun 19;48(11):6092-6107.
doi: 10.1093/nar/gkaa355.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin-α antibody, clone YOL1/34 (MCA78G) used to demonstratel microtubules in dividing Drosophila larvae by immunofluorescence.
Image caption:
Mutations in nebu lead to mitotic defects in early embryogenesis. (A) Embryos from females mutant for nebu (CG10960) exhibited aberrant mitotic divisions, resulting in loss of integral nuclear doublings and abnormal mitotic spindles, such as those shown in (B). Each image represents a different embryo of the designated genotype. Scale bar represents 100 μm. DNA is shown in magenta, and tubulin in green.
From: Avilés-Pagán EE, Kang ASW, Orr-Weaver TL. Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.
G3 (Bethesda). 2020 Sep 2;10(9):2989-98.
doi: 10.1534/g3.120.401415.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α tubulin antibody, clone YOL1/34 (MCA78G) used to demonstrate &alpha tubulin expression in HeLa cells by immunofluorescence.
Image caption:
Tyrosine phosphorylation on mitotic spindles. Taxol-stabilized mitotic spindles were isolated from cells released from nocodazole-induced arrest.
(a) Mitotic spindles isolated from parental HeLa cells (upper panels) and HeLa S3/Fyn cells (lower panels) were doubly stained for endogenous Fyn (red) plus α-tubulin (green) (upper panels) and expressed Fyn (red) plus α-tubulin (green) (lower panels). Scale bar, 10 μm.
From: Morii M, et al.
Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
Sci Rep. 2021 Jan 28;11(1):2616.
doi: 10.1038/s41598-021-82189-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α tubulin antibody, clone YOL1/34 (MCA78G) used to demonstrate &alpha tubulin expression in HeLa cells by immunofluorescence.
Image caption:
Tyrosine phosphorylation on mitotic spindles. Taxol-stabilized mitotic spindles were isolated from cells released from nocodazole-induced arrest. Mitotic spindles isolated from HeLa S3/Fyn cells were doubly stained for Fyn (red) plus α-tubulin (green) (upper panels) and pTyr (red) plus α-tubulin (green) (lower panels). Scale bars, 10 μm.
From: Morii M, et al.
Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
Sci Rep. 2021 Jan 28;11(1):2616.
doi: 10.1038/s41598-021-82189-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α tubulin antibody, clone YOL1/34 (MCA78G) used to demonstrate &alpha tubulin expression in HeLa cells by western blotting.
Image caption:
Tyrosine phosphorylation on mitotic spindles. Taxol-stabilized mitotic spindles were isolated from cells released from nocodazole-induced arrest. HeLa S3/Fyn cells, treated with or without 10 μM PP2 were lysed and analyzed by Western blotting using the indicated antibodies.
From: Morii M, et al.
Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
Sci Rep. 2021 Jan 28;11(1):2616.
doi: 10.1038/s41598-021-82189-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α tubulin antibody, clone YOL1/34 (MCA78G) used to demonstrate &alpha tubulin expression in HeLa cells by western blotting.
Image caption:
Tyrosine phosphorylation on mitotic spindles. Taxol-stabilized mitotic spindles were isolated from cells released from nocodazole-induced arrest. parental HeLa S3 and HeLa S3/Fyn cells, treated with or without 10 μM PP2 were lysed and analyzed by Western blotting using the indicated antibodies.
From: Morii M, et al.
Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
Sci Rep. 2021 Jan 28;11(1):2616.
doi: 10.1038/s41598-021-82189-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α tubulin antibody, clone YOL1/34 (MCA78G) used to demonstrate &alpha tubulin expression in HeLa cells by immunofluorescence.
Image caption:
The effects of the tyrosine phosphorylation of PRC1 at Tyr-464 on its association with microtubules. Parental HeLa S3/TR cells and HeLa S3/TR cells, which express myc-PRC1-wt, myc-PRC1-Y464E, or myc-PRC1-Y464F, were treated with Dox for inducible expression. Cells were treated with or without 1 μg/ml nocodazole for the last 30 min. Scale bars, 20 μm.
From: Morii M, et al.
Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
Sci Rep. 2021 Jan 28;11(1):2616.
doi: 10.1038/s41598-021-82189-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used to label microtubules in murine zygote preparations by immunofluorescence.
Image caption:
Three-dimensional images of zygotes expressing miRFP670-Trim21 and microinjected with Mouse-IgG or Pericentrin-IgG, fixed 5 hpi and stained for aMTOCs (anti-pericentrin), microtubules (anti-α-tubulin) and DNA (Hoechst) (z-projection of 11 sections, every 1 μm). The regions outlined in the overviews are magnified in bottom panel.
From: Scheffler K, Uraji J, Jentoft I, Cavazza T, Mönnich E, Mogessie B, Schuh M.
Two mechanisms drive pronuclear migration in mouse zygotes.
Nat Commun. 2021 Feb 5;12 (1): 841.
doi: 10.1038/s41467-021-21020-x.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α-tubulin antibody, clone YOL1/34 (MCA78G) used to visualize microtubules in dividing murine oocytes by immunofluorescence.
Image caption:
Dmc1−/−Puma−/−/−−Noxa−/− oocytes resume meiosis.
b, Representative images of chromosomes (Hoechst, magenta), kinetochores (CREST, white), and microtubules (α-tubulin, green) in metaphase I (z-projections every 0.19 μm) cold-treated oocytes from control (Dmc1+/− Puma+/− Noxa+/− or Dmc+/+ Puma+/+ Noxa+/+; n = 10 females), Dmc1+/+ Puma−/− Noxa−/− (n = 8 females) and Dmc1−/− Puma−/− Noxa−/− (n = 2 females). Insets show a magnification of a meiosis I bivalent chromosome (left and middle) or two prematurely dissociated univalent chromosomes (right). All oocytes preserved at metaphase I had all 20 chromosomes in either the bivalent or in the univalent morphology. Scale bar, 10 μm.
From: ElInati E, Zielinska AP, McCarthy A, Kubikova N, Maciulyte V, Mahadevaiah S, Sangrithi MN, Ojarikre O, Wells D, Niakan KK, Schuh M, Turner JMA.
The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes.
Nat Commun. 2020 May 25;11 (1): 2598.
doi: 10.1038/s41467-020-16441-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti α-tubulin antibody, clone YOL1/34 (MCA78G) used to visualize microtubules in dividing murine oocytes by immunofluorescence.
Image caption:
Dmc1−/−Puma−/−/−−Noxa−/− oocytes resume meiosis.
b, Representative images of chromosomes (Hoechst, magenta), kinetochores (CREST, white), and microtubules (α-tubulin, green) in metaphase II (z-projections every 0.19 μm) cold-treated oocytes from control (Dmc1+/− Puma+/− Noxa+/− or Dmc+/+ Puma+/+ Noxa+/+; n = 10 females), Dmc1+/+ Puma−/− Noxa−/− (n = 8 females) and Dmc1−/− Puma−/− Noxa−/− (n = 2 females). Insets show a magnification of a meiosis I bivalent chromosome (left and middle) or two prematurely dissociated univalent chromosomes (right). All oocytes preserved at metaphase I had all 20 chromosomes in either the bivalent or in the univalent morphology. Scale bar, 10 μm.
From: ElInati E, Zielinska AP, McCarthy A, Kubikova N, Maciulyte V, Mahadevaiah S, Sangrithi MN, Ojarikre O, Wells D, Niakan KK, Schuh M, Turner JMA.
The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes.
Nat Commun. 2020 May 25;11 (1): 2598.
doi: 10.1038/s41467-020-16441-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used to show microtubules in HeLa cells by immunofluorescence.
Image caption:
HeLa cells expressing GFP-BtpB (green) were labelled and analyzed by confocal immunofluorescence microscopy. Representative images are shown. Cells were labelled with anti-tubulin antibody for visualization of the microtubules (red). Scale bars correspond to 5 μm. The image was obtained with Airyscan confocal imaging mode.
From: Coronas-Serna JM, Louche A, Rodríguez-Escudero M, Roussin M, Imbert PRC, Rodríguez-Escudero I, et al.. (2020)
The TIR-domain containing effectors BtpA and BtpB from Brucella abortus impact NAD metabolism.
PLoS Pathog 16(4): e1007979.
doi: 10.1371/journal.ppat.1007979
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used to show microtubules in HeLa cells by immunofluorescence.
Image caption:
The HeLa cells were transfected with GFP-BtpB, GFP-BtpB-N (1–139), GFP-BtpB-TIR (140–292) and GFP-BtpB E234A. Cells were then labelled for tubulin (red). Scale bars correspond to 5 μm and all images obtained with Airyscan confocal imaging mode.
From: Coronas-Serna JM, Louche A, Rodríguez-Escudero M, Roussin M, Imbert PRC, Rodríguez-Escudero I, et al.. (2020)
The TIR-domain containing effectors BtpA and BtpB from Brucella abortus impact NAD metabolism.
PLoS Pathog 16(4): e1007979.
doi: 10.1371/journal.ppat.1007979
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used to show microtubules in HeLa cells by immunofluorescence.
Image caption:
Localization and effects of GFP-BtpB versions in HeLa cells.
(C) HeLa cells were transfected with GFP-BtpB-TIR (green) and then labelled for tubulin (red) . (E) Representative image of cells labelled with anti-tubulin antibody (red) expressing aggregates of GFP-BtpB E234A (green). (B) GFP-BtpB (green) can be detected at intercellular contacts (arrow and zoomed image). Cells were labeled with an anti-tubulin antibody (red). Scale bars correspond to 5 μm.
PLoS Pathog 16(4): e1007979.
doi: 10.1371/journal.ppat.1007979
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin-α antibody, clone YOL1/34 (MCA78G) used to label microtubules in mitotic HeLa cells by immunofluorescence.
Image caption:
Depletion of linc00899 causes chromosome congression defects.
Characterisation of mitotic phenotypes in HeLa cells after RNAi- and LNA-mediated depletion of linc00899 and C1QTNF1-AS1. Mitotic cells with (i) narrow metaphase plate (blue), (ii) wide metaphase plate (orange), (iii) congression defect I (dark pink), and (iv) congression defect II (brown) using α-tubulin (microtubules, in green) and CREST (kinetochore marker, in red). Scale bar, 5 μm.
From: Stojic L, Lun ATL, Mascalchi P, Ernst C, Redmond AM, Mangei J, Barr AR, Bousgouni V, Bakal C, Marioni JC, Odom DT, Gergely F.
A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.
Nat Commun. 2020 Apr 15;11(1):1851.
doi: 10.1038/s41467-020-14978-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Tubulin-α antibody, clone YOL1/34 (MCA78G) used to demonstrate microtubules in Asplenium nidus cells.
Image caption:
(a) Median paradermal section of a group of A. nidus protodermal cells, as seen in light microscope after toluidine blue staining. The asterisk marks a cell in cytokinesis. The arrows point to daughter cell walls of symmetrical divisions that are arranged on the same plane. The arrowheads show the cell plate of the cytokinetic cell. (b,c) Median optical sections of groups of A. nidus protodermal cells, where (b) MT and (c) ER membrane immunolabeling has taken place. The arrows point to profiles of (b) MT-PPBs and (c) ER-PPBs.
From: Giannoutsou E, Galatis B, Apostolakos P.
De-Esterified Homogalacturonan Enrichment of the Cell Wall Region Adjoining the Preprophase Cortical Cytoplasmic Zone in Some Protodermal Cell Types of Three Land Plants.
Int J Mol Sci. 2019 Dec 20;21(1):81.
doi: 10.3390/ijms21010081.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Tubulin-α antibody, clone YOL1/34 (MCA78G) used to demonstrate microtubules in Zea mays stomatal cells.
Image caption:
(a–g) Z. mays stomatal rows regions seen in (a‒d) DIC optics and (e‒g) after MT immunolabeling. Asterisks mark the GMCs, squares the SMCs and circles the nuclei. The double-headed arrow in (a) indicates the orientation of the leaf axis. (a) Part of a stomatal row near leaf meristem. The arrows point to the daughter cell walls of symmetrical divisions, while the arrowhead to the daughter cell wall of the asymmetrical division creating a GMC. (b) Newly-formed GMC. (c) GMC at the stage of induction of the adjacent SMCs becomes polarized and divides asymmetrically. The nucleus (circle) of the one SMC has been moved near the inducing GMC, while the other SMC has divided asymmetrically. The arrow points to the daughter cell wall of the asymmetrical division. (d) Newly-formed stomatal complex. The arrow shows the daughter cell wall of the symmetrical division of the GMC, while the arrowheads mark the daughter cell wall of the asymmetrical division separating the subsidiary cells. (e) Surface optical section of a preprophase cell of the stomatal row that is going to divide asymmetrically to create a GMC. The arrows indicate the MT-PPB. (f1,2) Optical sections passing through an (f1) external and (f2) a median plane of a preprophase SMC. The arrows point to the MT-PPB as seen in these two (f1,2) different planes. The circle marks the nucleus of the SMC. (g) Optical section through a median plane of a preprophase GMC. The arrows mark the MT-PPB. The arrowheads designate the MT-PPB in the adjacent SMC. The circle shows the nucleus of the SMC. Scale bars = 10 μm. Triangles are referred as arrowheads.
From: Giannoutsou E, Galatis B, Apostolakos P.
De-Esterified Homogalacturonan Enrichment of the Cell Wall Region Adjoining the Preprophase Cortical Cytoplasmic Zone in Some Protodermal Cell Types of Three Land Plants.
Int J Mol Sci. 2019 Dec 20;21(1):81.
doi: 10.3390/ijms21010081.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti tubulin alpha antibody, clone YOL1/34 (MCA78G) used to label microtubules in the seagrass Halophila stipulacea by immunofluorescence.
Image caption:
Maximum intensity projections of CLSM sections of cortical microtubules in control (A1) and Cr (VI)-treated Halophila stipulacea leaf cells (A2): A1. Abundant and unaligned cortical microtubules can be observed in the control. A2. At 10 mg L−1 (14d), cortical microtubules appear unaffected. Selected images from a set of at least three images per concentration and day. Scale bar: 10 μm
From: Malea P et al.
Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea.
Ecological Indicators 2020. 122: 107224.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB IF ResetRat anti Tubulin Alpha
- Product Type
- Monoclonal Antibody
- Clone
- YOL1/34
- Isotype
- IgG2a
- Specificity
- Tubulin Alpha
Quick Links:
Antibody Quality and 
Unrivaled 
| Rat anti Tubulin alpha antibody, clone YOL1/34 recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2. Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin. |
- Target Species
- Yeast
- Species Cross-Reactivity
-
Target Species Cross Reactivity Birds Expected from Sequence Mammals Expected from Sequence Drosophila Fungal Expected from Sequence Human Arabidopsis thaliana Saccharomyces Platyzoma Ashbya Mouse Naegleria Asplenium nidus Seagrass (Cymodocea nodosa) - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Yeast tubulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fuzed with cells of the rat YB2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
10ug/ml as detecting antibody | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Radioimmunoassays | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Tubulin Alpha antibody
-
Goto, G.H. et al. (2011) Bub1-mediated adaptation of the spindle checkpoint.
PLoS Genet. 7: e1001282. -
Fonseca, A.V. et al. (2010) Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network.
J Biol Chem. 285: 31661-71. -
Lang, C. et al. (2010) Mobility, microtubule nucleation and structure of microtubule-organizing centers in multinucleated hyphae of Ashbya gossypii.
Mol Biol Cell. 21: 18-28. -
Petronczki, M. et al. (2004) Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II.
J Cell Sci. 117: 3547-59. -
Rossio, V. et al. (2010) The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase
J Cell Biol. 191: 981-97. -
Takeo, S. et al. (2010) Calcineurin and its regulation by Sra/RCAN is required for completion of meiosis in Drosophila.
Dev Biol. 344: 957-67. -
Trieselmann, N. et al. (2003) Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation.
J Cell Sci. 116: 4791-8. -
Whelan, F. et al. (2010) Amino acid substitutions in the aryl hydrocarbon receptor ligand binding domain reveal YH439 as an atypical AhR activator.
Mol Pharmacol. 77: 1037-46.
View The Latest Product References
-
Hao, N. et al. (2013) Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation.
Nucleic Acids Res. 41 (11): 5626-38. -
Kilmartin, J.V. et al. (1982) Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
J Cell Biol. 93 (3): 576-82. -
Waples, W.G. et al. (2009) Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit.
Mol Biol Cell. 20 (1): 245-55. -
White, J. et al. (2005) Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation.
Mol Biol Cell. 16 (4): 2018-27. -
Lang, C. et al. (2010) Structural mutants of the spindle pole body cause distinct alteration of cytoplasmic microtubules and nuclear dynamics in multinucleated hyphae.
Mol Biol Cell. 21 (5): 753-66. -
Hao, N. et al. (2011) Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR.
Nucleic Acids Res. 39 (9): 3695-709. -
D'Ambrosio, C. et al. (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.
Genes Dev. 22: 2215-27 -
Hartl, T.A. et al. (2008) Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis.
PLoS Genet. 4: e1000228. -
Mirchenko, L. and Uhlmann, F. (2010) Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset.
Curr Biol. 20: 1396-401. -
Sullivan, M. et al. (2001) Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19.
Nat Cell Biol. 3: 771-7. -
Sullivan, M. et al. (2008) Cyclin-specific control of ribosomal DNA segregation.
Mol Cell Biol. 28: 5328-36. -
Yu, H.G. et al. (2007) The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
J Cell Biol. 176: 911-8. -
Rossio, V. and Yoshida, S. (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.
J Cell Biol. 193: 445-54. -
Grava, S. et al. (2011) Clustering of Nuclei in Multinucleated Hyphae Is Prevented by Dynein-Driven Bidirectional Nuclear Movements and Microtubule Growth Control in Ashbya gossypii.
Eukaryot Cell. 10: 902-15. -
Raspelli, E. et al. (2011) Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
Mol Biol Cell. 22: 2185-97. -
Finlayson, M.R. et al. (2011) Regulation of exit from mitosis in multinucleate Ashbya gossypii cells relies on a minimal network of genes.
Mol Biol Cell. 22 (17): 3081-93. -
Chatzimeletiou, K. et al. (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy.
Hum Reprod. 20: 672-82. -
Chatzimeletiou, K. et al. (2012) Cytoskeletal analysis of human blastocysts by confocal laser scanning microscopy following vitrification.
Hum Reprod. 27: 106-13. -
Keeling, J.W. and Miller, R.K. (2011) Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast.
Methods Mol Biol. 782: 231-44. -
Elhanany-Tamir, H. et al. (2012) Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules.
J Cell Biol. 198 (5): 833-46. -
Walsh, C.J. (2012) The structure of the mitotic spindle and nucleolus during mitosis in the amebo-flagellate Naegleria.
PLoS One. 7: e34763. -
Adamakis, I.D. et al. (2013) Effects of bisphenol A on the microtubule arrays in root meristematic cells of Pisum sativum L.
Mutat Res. 750 (1-2): 111-20. -
Buerstenbinder, K. et al. (2013) Arabidopsis Calmodulin-binding IQD1 Localizes to Microtubules and Interacts with Kinesin Light Chain-Related Protein-1.
J Biol Chem. 288: 1871-82. -
Panteris, E. et al. (2013) The distribution of TPX2 in dividing leaf cells of the fern Asplenium nidus.
Plant Biol (Stuttg). 15: 203-9. -
Malea, P. et al. (2013) Microtubule integrity and cell viability under metal (Cu, Ni and Cr) stress in the seagrass Cymodocea nodosa.
Chemosphere. pii: S0045-6535(13)00820-5. -
Eleftheriou, E.P. et al. (2016) Hexavalent chromium-induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of α-tubulin.
Protoplasma. 253 (2): 531-42. -
Wang, S. et al. (2015) Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1.
J Cell Biol. 209 (4): 529-38. -
Okamoto, M. et al. (2016) Fyn Accelerates M Phase Progression by Promoting the Assembly of Mitotic Spindle Microtubules.
J Cell Biochem. 117 (4): 894-903. -
Eleftheriou, E.P. et al. (2015) Aberration of mitosis by hexavalent chromium in some Fabaceae members is mediated by species-specific microtubule disruption.
Environ Sci Pollut Res Int. 22 (10): 7590-9. -
Sullivan, A.E. et al. (2016) MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors.
FEBS J. 283 (18): 3488-502. -
Schweizer, N. et al. (2015) An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.
J Cell Biol. 210 (5): 695-704. -
Livanos P et al. (2016) Deliberate ROS production and auxin synergistically trigger the asymmetrical division generating the subsidiary cells in Zea mays stomatal complexes.
Protoplasma. 253 (4): 1081-99. -
Xie, J.L. et al. (2017) Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2 (2): Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr. -
Bacon, T. et al. (2015) Histone deacetylase 3 indirectly modulates tubulin acetylation.
Biochem J. 472 (3): 367-77. -
Kowanda, M. et al. (2016) Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.
Biol Open. 5 (8): 1040-51. -
Diao, L.T. et al. (2017) Delineation of the role of chromatin assembly and the Rtt101Mms1 E3 ubiquitin ligase in DNA damage checkpoint recovery in budding yeast.
PLoS One. 12 (7): e0180556. -
Játiva, S. et al. (2019) Cdc14 activation requires coordinated Cdk1-dependent phosphorylation of Net1 and PP2A-Cdc55 at anaphase onset.
Cell Mol Life Sci. 76 (18): 3601-20. -
Jühlen, R. et al. (2020) Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts.
Sci Rep. 10 (1): 19301. -
Yam, C.Q.X. et al. (2020) Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.
Nucleic Acids Res. 48 (11): 6092-107. -
Avilés-Pagán, E.E. et al. (2020) Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.
G3 (Bethesda). 10 (9): 2989-2998. -
Morii, M. et al. (2021) Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle.
Sci Rep. 11 (1): 2616. -
Scheffler, K. et al. (2021) Two mechanisms drive pronuclear migration in mouse zygotes.
Nat Commun. 12 (1): 841. -
ElInati, E. et al. (2020) The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes.
Nat Commun. 11 (1): 2598. -
Coronas-Serna, J.M. et al. (2020) The TIR-domain containing effectors BtpA and BtpB from Brucella abortus impact NAD metabolism.
PLoS Pathog. 16 (4): e1007979. -
Stojic, L. et al. (2020) A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.
Nat Commun. 11 (1): 1851. -
Giannoutsou, E. et al. (2019) De-Esterified Homogalacturonan Enrichment of the Cell Wall Region Adjoining the Preprophase Cortical Cytoplasmic Zone in Some Protodermal Cell Types of Three Land Plants.
Int J Mol Sci. 21(1):81. -
Cavazza, T. et al. (2021) Parental genome unification is highly error-prone in mammalian embryos.
Cell. Apr 30 [Epub ahead of print]. -
Panteris, E. et al. (2018) Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin.
J Biol Res (Thessalon). 25: 12. -
Giourieva, V. & Panteris, E. (2021) Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana..
J Biol Res (Thessalon). 28 (1): 13. -
Malea, P. et al. (2021) Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea..
Ecological Indicators. 122: 107224.
- RRID
- AB_325005
View more products with TUBULIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Yeast ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up