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Tubulin Alpha antibody | YOL1/34

Rat anti Tubulin Alpha

Product Type
Monoclonal Antibody
Tubulin Alpha

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF R WB 0.5 mg loader
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Rat anti tubulin alpha antibody, clone YOL1/34 recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with clone YL1/2.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Birds Expected from Sequence
Mammals Expected from Sequence
Fungal Expected from Sequence
Arabidopsis thaliana
Asplenium nidus
Seagrass (Cymodocea nodosa)
Rye (Secale cereale L.)
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yeast tubulin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized LOU rats were fused with cells of the rat YB2/0 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 10ug/ml as detecting antibody
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Source Reference

  1. Kilmartin, J.V. et al. (1982) Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
    J Cell Biol. 93 (3): 576-82.

References for Tubulin Alpha antibody

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  4. Chatzimeletiou, K. et al. (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy.
    Hum Reprod. 20: 672-82.
  5. White, J. et al. (2005) Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation.
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  9. View The Latest Product References
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    Hum Reprod. 27: 106-13.
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    Plant Biol (Stuttg). 15: 203-9.
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    Environ Sci Pollut Res Int. 22 (10): 7590-9.
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Western Blotting



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