Tubulin Alpha antibody | YOL1/34















Rat anti Tubulin alpha antibody, clone YOL1/34 is routinely tested in ELISA on Tubulin.
Product Details
- Target Species
- Yeast
- Species Cross-Reactivity
-
Target Species Cross Reactivity Birds Expected from Sequence Mammals Expected from Sequence Drosophila Fungal Expected from Sequence Human Arabidopsis Saccharomyces Platyzoma Ashbya Mouse Naegleria Asplenium nidus Seagrass (Cymodocea nodosa) - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Yeast tubulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fuzed with cells of the rat YB2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Tubulin Alpha antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 10ug/ml as detecting antibody | ||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Radioimmunoassays | |||
Western Blotting |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Product Specific References
References for Tubulin Alpha antibody
-
Kilmartin, J.V. et al. (1982) Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
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White, J. et al. (2005) Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation.
Mol Biol Cell. 16 (4): 2018-27. -
Lang, C. et al. (2010) Structural mutants of the spindle pole body cause distinct alteration of cytoplasmic microtubules and nuclear dynamics in multinucleated hyphae.
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Hao, N. et al. (2011) Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR.
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D'Ambrosio, C. et al. (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes.
Genes Dev. 22: 2215-27 -
Hartl, T.A. et al. (2008) Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis.
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Mirchenko, L. and Uhlmann, F. (2010) Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset.
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Sullivan, M. et al. (2001) Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19.
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Sullivan, M. et al. (2008) Cyclin-specific control of ribosomal DNA segregation.
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Yu, H.G. et al. (2007) The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
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Rossio, V. and Yoshida, S. (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.
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Grava, S. et al. (2011) Clustering of Nuclei in Multinucleated Hyphae Is Prevented by Dynein-Driven Bidirectional Nuclear Movements and Microtubule Growth Control in Ashbya gossypii.
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Raspelli, E. et al. (2011) Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
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Finlayson, M.R. et al. (2011) Regulation of exit from mitosis in multinucleate Ashbya gossypii cells relies on a minimal network of genes.
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Chatzimeletiou, K. et al. (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy.
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Chatzimeletiou, K. et al. (2012) Cytoskeletal analysis of human blastocysts by confocal laser scanning microscopy following vitrification.
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Keeling, J.W. and Miller, R.K. (2011) Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast.
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Elhanany-Tamir, H. et al. (2012) Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules.
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Walsh, C.J. (2012) The structure of the mitotic spindle and nucleolus during mitosis in the amebo-flagellate Naegleria.
PLoS One. 7: e34763. -
Adamakis, I.D. et al. (2013) Effects of bisphenol A on the microtubule arrays in root meristematic cells of Pisum sativum L.
Mutat Res. 750 (1-2): 111-20. -
Buerstenbinder, K. et al. (2013) Arabidopsis Calmodulin-binding IQD1 Localizes to Microtubules and Interacts with Kinesin Light Chain-Related Protein-1.
J Biol Chem. 288: 1871-82. -
Panteris, E. et al. (2013) The distribution of TPX2 in dividing leaf cells of the fern Asplenium nidus.
Plant Biol (Stuttg). 15: 203-9. -
Malea, P. et al. (2013) Microtubule integrity and cell viability under metal (Cu, Ni and Cr) stress in the seagrass Cymodocea nodosa.
Chemosphere. pii: S0045-6535(13)00820-5. -
Eleftheriou, E.P. et al. (2016) Hexavalent chromium-induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of α-tubulin.
Protoplasma. 253 (2): 531-42. -
Wang, S. et al. (2015) Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1.
J Cell Biol. 209 (4): 529-38. -
Okamoto, M. et al. (2016) Fyn Accelerates M Phase Progression by Promoting the Assembly of Mitotic Spindle Microtubules.
J Cell Biochem. 117 (4): 894-903. -
Eleftheriou, E.P. et al. (2015) Aberration of mitosis by hexavalent chromium in some Fabaceae members is mediated by species-specific microtubule disruption.
Environ Sci Pollut Res Int. 22 (10): 7590-9. -
Sullivan, A.E. et al. (2016) MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors.
FEBS J. 283 (18): 3488-502. -
Schweizer, N. et al. (2015) An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.
J Cell Biol. 210 (5): 695-704. -
Livanos P et al. (2016) Deliberate ROS production and auxin synergistically trigger the asymmetrical division generating the subsidiary cells in Zea mays stomatal complexes.
Protoplasma. 253 (4): 1081-99. -
Xie, J.L. et al. (2017) Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A.
mSphere. 2 (2): Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr. -
Bacon, T. et al. (2015) Histone deacetylase 3 indirectly modulates tubulin acetylation.
Biochem J. 472 (3): 367-77. -
Kowanda, M. et al. (2016) Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.
Biol Open. 5 (8): 1040-51. -
Diao, L.T. et al. (2017) Delineation of the role of chromatin assembly and the Rtt101Mms1 E3 ubiquitin ligase in DNA damage checkpoint recovery in budding yeast.
PLoS One. 12 (7): e0180556. -
Játiva, S. et al. (2019) Cdc14 activation requires coordinated Cdk1-dependent phosphorylation of Net1 and PP2A-Cdc55 at anaphase onset.
Cell Mol Life Sci. 76 (18): 3601-20. -
Jühlen, R. et al. (2020) Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts.
Sci Rep. 10 (1): 19301. -
Yam, C.Q.X. et al. (2020) Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.
Nucleic Acids Res. 48 (11): 6092-107. -
Avilés-Pagán, E.E. et al. (2020) Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.
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