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Tubulin Alpha antibody | YL1/2

Rat anti Tubulin Alpha

Product Type
Monoclonal Antibody
Clone
YL1/2
Isotype
IgG2a
Specificity
Tubulin Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA77G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF IP R WB 0.5 mg loader
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loader

Rat anti tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).

The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).

Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.

Target Species
Yeast
Species Cross-Reactivity
Target SpeciesCross Reactivity
Ashbya
Birds Expected from Sequence
Echinoderm Expected from Sequence
Human
Mouse
Dog
Rat
Pig
Drosophila
Plants Expected from Sequence
Amphibia Expected from Sequence
Saccharomyces
Pleurobrachia
Caenorhabditis
Dictyostelium discoideum
Xenopus
Pig-tailed macaque
Clytia sp.
Arabidopsis
Strongylocentrotus purpuratus
Dendraster excentricus
Trypanosoma brucei
Potorous tridactylis
Bovine
Nephrotoma suturalis
Hemicentrotus pulcherrimus
Potato
Bombyx mori
Rhodnius prolixus
Beroe abyssicola
Candida sp.
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Yeast tubulin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Radioimmunoassays
Western Blotting 1/200 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
Rat anti tubulin alpha antibody, clone YL1/2 recognizes a band of ~55 kDa in cell lysate from a wide range of species. Rat anti tubulin alpha antibody, clone YL1/2 is suitable for use as a western blotting loading control.

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References for Tubulin Alpha antibody

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RRID
AB_325003

MCA77G

147397 155610 157801 161496 162983 163862 165044 165568

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