Tubulin Alpha antibody | YL1/2
Rat anti Tubulin Alpha
- Product Type
- Monoclonal Antibody
- Clone
- YL1/2
- Isotype
- IgG2a
- Specificity
- Tubulin Alpha
Rat anti tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987). Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin. |
- Target Species
- Yeast
- Species Cross-Reactivity
-
Target Species Cross Reactivity Ashbya Birds Expected from Sequence Echinoderm Expected from Sequence Human Mouse Dog Rat Pig Drosophila Plants Expected from Sequence Amphibia Expected from Sequence Saccharomyces Pleurobrachia Caenorhabditis Dictyostelium discoideum Xenopus Pig-tailed macaque Clytia sp. Arabidopsis Strongylocentrotus purpuratus Dendraster excentricus Trypanosoma brucei Potorous tridactylis Bovine Nephrotoma suturalis Hemicentrotus pulcherrimus Potato Bombyx mori Rhodnius prolixus Beroe abyssicola Candida sp. - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Yeast tubulin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100 | 1/1000 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Radioimmunoassays | |||
Western Blotting | 1/200 | 1/2000 |
- Western Blotting
- Rat anti tubulin alpha antibody, clone YL1/2 recognizes a band of ~55 kDa in cell lysate from a wide range of species. Rat anti tubulin alpha antibody, clone YL1/2 is suitable for use as a western blotting loading control.
References for Tubulin Alpha antibody
-
Kilmartin, J.V. et al. (1982) Rat monoclonal anti tubulin antibodies derived by using a new nonsecreting rat cell line.
J Cell Biol. 93 (3): 576-82. -
Wehland, J. et al. (1983) A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo.
J Cell Biol. 97 (5 Pt 1): 1467-75. -
Wehland, J. et al. (1984) Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.
EMBO J. 3 (6): 1295-300. -
Burns, R. (1987) Cytoskeleton. Tubulin's terminal tyrosine.
Nature. 327 (6118): 103-4. -
Skinner, R.H. et al. (1991) Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins.
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Berrueta, L. et al. (1998) The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules.
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Gordon-Weeks, R. et al. (2003) Restricted spatial expression of a high-affinity phosphate transporter in potato roots.
J Cell Sci.116: 3135-44. -
Ligon, L.A. et al. (2003) The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.
Mol Biol Cell. 14: 1405-17.
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Genes Cells. Jun 25 [Epub ahead of print].
- RRID
- AB_325003
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