M13 Bacteriophage antibody | E1
Mouse anti M13 Bacteriophage antibody, clone E1 recognizes the gp3 (G3P) phage coat protein of the M13 bacteriophage widely used in the preparation of 'phage-display' immunoglobulin libraries. G3P plays a role in the entry of the viral genome into the bacterial host..
This antibody may be used to detect binding of ScFv antibodies developed in the M13 system.
This antibody may be used to detect binding of ScFv antibodies developed in the M13 system.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.08% Sodium Azide - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- M13 Phage coat proteins.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of M13 Bacteriophage antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | 5ug/ml | |
| Flow Cytometry | |||
| ELISA | |||
| Flow Cytometry | 10ug/ml | ||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
|
|
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
|
Product Specific References
References for M13 Bacteriophage antibody
-
Jaye, D.L.et al. (2003) Use of real-time polymerase chain reaction to identify cell- and tissue-type-selective
peptides by phage display.
Am J Pathol. 162:1419-29.
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