M13 Bacteriophage antibody | E1

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Mouse anti M13 Bacteriophage:Biotin

Mouse anti M13 Bacteriophage

Product Type
Monoclonal Antibody
Clone
E1
Isotype
IgG3
Product Code Applications Pack Size List Price Quantity
MCA1858B
Datasheet
E F
MSDS
0.1 mg loader

MCA1858R
Datasheet
C E F
MSDS
0.25 mg loader

Mouse anti M13 Bacteriophage antibody, clone E1 recognizes the gp3 (G3P) phage coat protein of the M13 bacteriophage widely used in the preparation of 'phage-display' immunoglobulin libraries. G3P plays a role in the entry of the viral genome into the bacterial host..

This antibody may be used to detect binding of ScFv antibodies developed in the M13 system.

Product Details

Target Species
Viral
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.08%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
M13 Phage coat proteins.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of M13 Bacteriophage antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 5ug/ml
Flow Cytometry
ELISA
Flow Cytometry 10ug/ml
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for M13 Bacteriophage antibody

  1. Jaye, D.L.et al. (2003) Use of real-time polymerase chain reaction to identify cell- and tissue-type-selective peptides by phage display.
    Am J Pathol. 162:1419-29.