M13 Bacteriophage antibody | E1

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Anti M13 Bacteriophage Antibody, clone E1 thumbnail image 1

Anti M13 Bacteriophage Antibody, clone E1 gallery image 1 — Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10 ug/ml anti-M13 antibody (**MCA1858R**) followed by rabbit anti-mouse IgG:FITC (STAR9B)

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Mouse anti M13 Bacteriophage

Product Type: Monoclonal Antibody
Clone: E1
Isotype: IgG3
Specificity: M13 Bacteriophage

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Secondary Antibodies
References (1)
Applications
Storage Information

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA1858R Datasheet Datasheet SDS SDS	C E F	0.25 mg		Log in	Get Quote
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Mouse anti M13 Bacteriophage antibody, clone E1 recognizes the gp3 (G3P) phage coat protein of the M13 bacteriophage widely used in the preparation of 'phage-display' immunoglobulin libraries. G3P plays a role in the entry of the viral genome into the bacterial host..

This antibody may be used to detect binding of ScFv antibodies developed in the M13 system.

Target Species: Viral
Product Form: Purified IgG - liquid
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: M13 Phage coat proteins.
Approx. Protein Concentrations: 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry			10ug/ml
Immunohistology - Frozen

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Description	Product Code	Applications	Pack Size	Your Price
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
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Description	Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
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Description	Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote
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Description	Rabbit F(ab')2 anti Mouse IgG:FITC

References for M13 Bacteriophage antibody

Jaye, D.L.et al. (2003) Use of real-time polymerase chain reaction to identify cell- and tissue-type-selective peptides by phage display.
Am J Pathol. 162:1419-29.