Ustekinumab antibody | AbD17827_hIgG1



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E ResetHuman anti Ustekinumab:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD17827_hIgG1
- Isotype
- IgG1
- Specificity
- Ustekinumab
Human Anti-Ustekinumab Antibody, clone AbD17827_hIgG1 is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug ustekinumab and inhibits binding to its target. The antibody can be used to measure the levels of ustekinumab and biosimilar products in bioanalytical assays. Clone AbD17827_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. Additionally it can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody is recommended as the detection antibody, paired with an anti-ustekinumab antibody in monovalent Fab format as the capture antibody, clone AbD17829 (HCA208) or clone AbD18334 (HCA209). Ustekinumab (Stelara) is a fully human monoclonal antibody drug (IgG1/kappa) that has been approved in US, Canada, and Europe for the treatment of plaque psoriasis and psoriatic arthritis. The FDA approved the medication for the treatment of moderate-severe Crohn's disease in 2016. The drug blocks interleukin IL-12 and IL-23 by binding with high affinity and specificity to the p40 subunit, preventing the binding of these two cytokines to T-lymphocytes, and thereby disrupting the proinflammatory pathway. View a summary of all anti-ustekinumab antibodies |
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- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Ustekinumab
- Affinity
- The monovalent intrinsic affinity of this antibody is KD=0.2 nM as measured by real time, label-free molecular interaction analysis on immobilized ustekinumab.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
Protocol: PK bridging ELISA to measure free drug.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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Description | Hispec Assay Diluent | ||||||
Human anti Ustekinumab | HCA208 | E | 0.1 mg |
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Description | Human anti Ustekinumab | ||||||
Human anti Ustekinumab | HCA209 | E | 0.1 mg |
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List Price | Your Price | ||||||
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Description | Human anti Ustekinumab |
References for Ustekinumab antibody
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Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1
- Synonyms
- Stelara®
HCA210P


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