Ustekinumab antibody | AbD17827_hIgG1



Human anti Ustekinumab:HRP
Anti-ustekinumab antibody is a recombinant, high affinity anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assay development for ustekinumab and biosimilars. It is recommended as the detection antibody in PK bridging ELISA format with HCA208 or HCA209 as capture antibody.
Human anti Ustekinumab
Anti-ustekinumab antibody is a recombinant, high affinity anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assay development for ustekinumab and biosimilars. It is recommended as the detection antibody in PK bridging ELISA format with HCA208 or HCA209 as capture antibody.
- Product Type
- Monoclonal Antibody
- Clone
- AbD17827_hIgG1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA210P | E | 0.1 mg |
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HCA210 | E | 0.1 mg |
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HCA210G | E | 1 mg |
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Product Details
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized ustekinumab.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.5 mg/ml.Pack Size: 1 mgIgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Ustekinumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
Protocol: PK bridging ELISA to measure free drug - ELISA
- This product may be used in a direct ELISA or as a detection antibody, in its HRP conjugated format, in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
Protocol: ADA bridging ELISA
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Ustekinumab | HCA208 | E | 0.1 mg |
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Human anti Ustekinumab | HCA209 | E | 0.1 mg |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Ustekinumab | HCA208 | E | 0.1 mg |
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Human anti Ustekinumab | HCA209 | E | 0.1 mg |
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LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
Product Specific References
References for Ustekinumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1
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