Trastuzumab antibody | AbD18141



Human anti Trastuzumab
Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated as a capture antibody in a PK bridging ELISA format with HCA176P.
- Product Type
- Monoclonal Antibody
- Clone
- AbD18141
- Isotype
- HuCAL Fab monovalent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA169 | E | 0.1 mg |
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HCA169G | E | 1 mg |
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Human Anti-Trastuzumab Antibody, clone AbD18141 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture antibody paired with antibody clone AbD16712_hIgG1 (HCA176P) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.
Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.
View a summary of all anti-trastuzumab antibodies.
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Trastuzumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Herceptin is a registered trademark of Genentech, Inc.His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Trastuzumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with HCA176P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
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Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
HuCAL Fab-FH Negative Control | HCA045 | E | 0.1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Trastuzumab | HCA166 | E | 0.1 mg |
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Human anti Trastuzumab | HCA167 | E | 0.1 mg |
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Human anti Trastuzumab | HCA168 | E | 0.1 mg |
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Human anti Trastuzumab | HCA176 | E | 0.1 mg |
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Human anti Trastuzumab | HCA177 | E | 0.1 mg |
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Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
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Product Specific References
References for Trastuzumab antibody
-
Bults, P. et al. (2016) LC-MS/MS-Based Monitoring of In Vivo Protein Biotransformation: Quantitative Determination of Trastuzumab and Its Deamidation Products in Human Plasma.
Anal Chem. 88 (3): 1871-7. -
Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
Anal Biochem. 540-541: 30-7.
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