Trastuzumab antibody | AbD18141
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% milk powder, Human Anti-Trastuzumab Antibody clone AbD18141 (HCA169) was added at a concentration of 2 μg/ml. Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Human Anti-Trastuzumab Antibody, clone AbD18141 (HCA169) at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, increasing concentrations of trastuzumab in PBST was added. Detection was performed using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data is presented as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Trastuzumab Antibody, clone AbD16712 (HCA166) (black) and Human Anti-Trastuzumab Antibody, clone AbD18141 (HCA169) (red) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using Human Anti-Trastuzumab:HRP Antibody, clone AbD16712_hIgG1 (HCA176P) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Human anti Trastuzumab
Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated as a capture antibody in a PK bridging ELISA format with HCA176P.
- Product Type
- Monoclonal Antibody
- Clone
- AbD18141
- Isotype
- HuCAL Fab monovalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA169 | E | 0.1 mg |
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| HCA169G | E | 1 mg |
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Human anti-Trastuzumab Antibody, clone AbD18141, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.
Human Anti-Trastuzumab Antibody, clone AbD18141 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture antibody paired with antibody clone AbD16712_hIgG1 (HCA176P) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.
Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.
View a summary of all anti-trastuzumab antibodies.
Human Anti-Trastuzumab Antibody, clone AbD18141 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture antibody paired with antibody clone AbD16712_hIgG1 (HCA176P) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.
Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.
View a summary of all anti-trastuzumab antibodies.
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Trastuzumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Herceptin is a registered trademark of Genentech, Inc.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Trastuzumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with HCA176P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
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| Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg |
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| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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| Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
|
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| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
|
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| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-FH Negative Control | HCA045 | E | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Trastuzumab | HCA166 | E | 0.1 mg |
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| Human anti Trastuzumab | HCA167 | E | 0.1 mg |
|
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| Human anti Trastuzumab | HCA168 | E | 0.1 mg |
|
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| Human anti Trastuzumab | HCA176 | E | 0.1 mg |
|
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| Human anti Trastuzumab | HCA177 | E | 0.1 mg |
|
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| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
|
Product Specific References
References for Trastuzumab antibody
-
Bults, P. et al. (2016) LC-MS/MS-Based Monitoring of In Vivo Protein Biotransformation: Quantitative Determination of Trastuzumab and Its Deamidation Products in Human Plasma.
Anal Chem. 88 (3): 1871-7. -
Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
Anal Biochem. 540-541: 30-7.
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