Trastuzumab antibody | AbD18018_hIgG4_Pro

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Human anti Trastuzumab

Anti-trastuzumab antibody is a recombinant, inhibitory, high affinity anti-idiotypic antibody in fully human immunoglobulin format (IgG4 isotype) for bioanalytical method development. It is recommended as the detection antibody in a PK bridging ELISA.

Product Type
Monoclonal Antibody
Clone
AbD18018_hIgG4_Pro
Isotype
IgG4
Product Code Applications Pack Size List Price Quantity
HCA270
Datasheet
E
MSDS
0.1 mg loader

Human anti trastuzumab, clone AbD18018_hIgG4_Pro, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. It is a fully human recombinant monoclonal antibody with IgG4 isotype. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.

Anti-trastuzumab antibody clone AbD18018 has been converted to human IgG4 isotype to offer assay developers increased flexibility in assay design. When used as an ELISA detection antibody, a secondary anti-human IgG4 antibody is required, which can be labeled according to the requirements of the user's preferred assay platform.

The antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug in preclinical research and clinical trials using patient sera. Antibody clones AbD16712 (HCA166) or AbD18141 (HCA169), both monovalent Fab format, are recommended as the capture antibody. Detection can be performed using clone AbD18018_hIgG4_Pro and an HRP conjugated anti-human IgG4 secondary antibody (MCA2098P).

This clone is also available as an IgG1 isotype in both a purified unconjugated format (HCA177) and directly conjugated to HRP (HCA177P).

Trastuzumab, also known as Herceptin®, is a drug used in the treatment of HER2 postive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

View a summary of all anti-trastuzumab antibodies

Product Details

Product Form
Human IgG4 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Trastuzumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.02 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Trastuzumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with an HRP conjugated anti human IgG4 secondary antibody (MCA2098P) and HCA169 as the capture reagent.

Protocol: In preparation.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human IgG4:HRP MCA2098P C E P 0.2 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Trastuzumab HCA166 E 0.1 mg loader
Human anti Trastuzumab HCA169 E 0.1 mg loader
Human anti Trastuzumab HCA177 E 0.1 mg loader
Human anti Trastuzumab:HRP HCA177P E 0.1 mg loader