Trastuzumab antibody | AbD18018_hIgG4_Pro
A microtiter plate was coated over night with Human anti trastuzumab, clone AbD18141 (HCA169) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum spiked with increasing concentrations of trastuzumab was added. Detection was performed using either HRP conjugated Human anti trastuzumab, clone AbD18018_hIgG4_Pro (HCA270) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) (black), or clone AbD18018_hIgG4Pro at 2 μg/ml followed by Mouse anti-human IgG4:HRP (MCA2098P) at a 1:10,000 dilution (green) in HISPEC assay diluent; QuantaBlu fluorogenic peroxidase substrate was used
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% milk powder, detection was performed using Human Anti-Trastuzumab Antibody, clone AbD18018_hIgG4_Pro (HCA270) at a concentration of 2 μg/ml followed by Mouse Anti-Human IgG4:HRP Antibody (MCA2098P) at 1:25,600 dilution in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Filter by Application:
E ResetHuman anti Trastuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD18018_hIgG4_Pro
- Isotype
- IgG4
- Specificity
- Trastuzumab
Antibody Quality and Performance Guarantee
| Human anti trastuzumab, clone AbD18018_hIgG4_Pro, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. It is a fully human recombinant monoclonal antibody with IgG4 isotype. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays. Anti-trastuzumab antibody clone AbD18018 has been converted to human IgG4 isotype to offer assay developers increased flexibility in assay design. When used as an ELISA detection antibody, a secondary anti-human IgG4 antibody is required, which can be labeled according to the requirements of the user's preferred assay platform. The antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug in preclinical research and clinical trials using patient sera. Antibody clones AbD16712 (HCA166) or AbD18141 (HCA169), both monovalent Fab format, are recommended as the capture antibody. Detection can be performed using clone AbD18018_hIgG4_Pro and an HRP conjugated anti-human IgG4 secondary antibody (MCA2098P). This clone is also available as an IgG1 isotype in both a purified unconjugated format (HCA177) and directly conjugated to HRP (HCA177P). Trastuzumab, also known as Herceptin®, is a drug used in the treatment of HER2 postive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells. View a summary of all anti-trastuzumab antibodies |
- Product Form
- Human IgG4 antibody selected from the HuCAL phage display library and expressed in a human cell line - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Trastuzumab
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=0.02 nM as measured by real time, label-free molecular interaction analysis on immobilized trastuzumab.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA together with an HRP conjugated anti human IgG4 secondary antibody (MCA2098P) and HCA169 as the capture reagent.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human IgG4:HRP | MCA2098P | C E P | 0.2 mg |
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| Description | Mouse anti Human IgG4:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Trastuzumab | HCA166 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA169 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA177 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab:HRP | HCA177P | E | 0.1 mg |
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| Description | Human anti Trastuzumab:HRP | ||||||
- Synonyms
- Herceptin
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA270
149997
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