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IgG4 antibody | HP6025

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Anti Human IgG4 Antibody, clone HP6025 thumbnail image 1 Anti Human IgG4 Antibody, clone HP6025 thumbnail image 2
Anti Human IgG4 Antibody, clone HP6025 gallery image 1
Published customer image:
Horseradish peroxidase conjugated Mouse anti Human IgG4 antibody, clone HP6025 (MCA2098P) used as a detection reagent for the assessment of MuSK IgG4 responses as measured by ELISA
Image caption:
linical severity and serological data of five patients with MuSK-MG from Greece before and after immunosuppressive treatment. (A) Clinical severity by QMG score, (B) IgG concentrations measured by ELISA, (C) IgG4 concentrations measured by nephelometry, (D) MGFA scores. Pharmacological remission is indicated as 0. (E) MuSK IgG concentration assessed by RIA, (F) MuSK IgG4 levels measured by ELISA. Colors indicate individual patients: pink = G1, blue = G2, red = G3, green = G4, and orange =G5. Because of the limited serum volume, the IgG4 of patient G3 and the MuSK IgG of patient G4 could not be assessed. Statistical analysis was not considered appropriate due to the low sample number.

From: Koneczny I, Mané-Damas M, Zong S, De Haas S, Huda S, van Kruining D, Damoiseaux J, De Rosa A, Maestri M, Guida M, Molenaar P, Van Damme P, Fichtenbaum A, Perkmann T, De Baets M, Lazaridis K, Zouvelou V, Tzartos S, Ricciardi R, Losen M, Martinez-Martinez P.
A retrospective multicenter study on clinical and serological parameters in patients with MuSK myasthenia gravis with and without general immunosuppression.
Front Immunol. 2024 Apr 23;15:1325171.
doi: 10.3389/fimmu.2024.1325171.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human IgG4 Antibody, clone HP6025 gallery image 2
Published customer image:
Horseradish peroxidase conjugated Mouse anti Human IgG4 antibody, clone HP6025 (MCA2098P) used as a detection reagent for the assessment of MuSK IgG4 responses as measured by ELISA
Image caption:
MuSK IgG4 levels correlated with clinical severity, but not with treatment status. MuSK IgG4 concentrations were measured by ELISA. (A) MuSK IgG4 concentrations (OD450) correlated with clinical severity (MGFA score). Linear regression followed by correlation with Spearman correlation coefficient. (B) MuSK IgG4 concentrations in patients with and without immunosuppressive treatments. Patients from Greece are indicated by blue symbols. Patients who remained untreated >6 months after disease onset are indicated by orange symbols. ns= non-significant. N = 3. Mann–Whitney test.

From: Koneczny I, Mané-Damas M, Zong S, De Haas S, Huda S, van Kruining D, Damoiseaux J, De Rosa A, Maestri M, Guida M, Molenaar P, Van Damme P, Fichtenbaum A, Perkmann T, De Baets M, Lazaridis K, Zouvelou V, Tzartos S, Ricciardi R, Losen M, Martinez-Martinez P.
A retrospective multicenter study on clinical and serological parameters in patients with MuSK myasthenia gravis with and without general immunosuppression.
Front Immunol. 2024 Apr 23;15:1325171.
doi: 10.3389/fimmu.2024.1325171.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human IgG4:HRP

Product Type
Monoclonal Antibody
Clone
HP6025
Isotype
IgG1
Specificity
IgG4

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Product Code Applications Pack Size List Price Your Price Qty
MCA2098P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P 0.2 mg loader
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loader

Mouse anti Human IgG4 antibody, clone HP6025 recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).

Elevated levels of IgG4 and of IgG4 presenting plasma cells are frequently seen in patients with autoimmune pancreatitis (IAP) and inflammatory bowel disease (Navaneethan et al. 2011) and it is suggested that IAP may develop as a paraneoplastic syndrome in some cancer patients (Shiokawa et al. 2013)

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to horseradish peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified IgG4
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with SP2/0 - Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/20000
Immunohistology - Frozen 1/40 1/80
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for IgG4 antibody

  1. Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.
    Immunol Lett. 10 (3-4): 223-52.
  2. Black, C.M. et al. (1991) Human markers for IgG2 and IgG4 appear to be on the same molecule in the chimpanzee.
    Immunology.72: 94-8.
  3. Yamashita, K. et al. (2008) Degree of IgG4+ plasma cell infiltration in retroperitoneal fibrosis with or without multifocal fibrosclerosis
    Histopathology. 52: 404-9.
  4. Yamashita, K. et al. (2008) Lung involvement in IgG4-related lymphoplasmacytic vasculitis and interstitial fibrosis: report of 3 cases and review of the literature.
    Am J Surg Pathol. 32: 1620-6.
  5. Miyagawa-Hayashino, A. et al. (2009) High ratio of IgG4-positive plasma cell infiltration in cutaneous plasmacytosis--is this a cutaneous manifestation of IgG4-related disease?
    Hum Pathol. 40: 1269-77.
  6. Fernandez-Becerra, C. (2010) Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay.
    Malar J. 9: 29.
  7. Agaimy, A. et al. (2010) Calcifying fibrous tumor of the stomach: clinicopathologic and molecular study of seven cases with literature review and reappraisal of histogenesis.
    Am J Surg Pathol. 34: 271-8.
  8. Navaneethan, U. et al. (2011) Tissue infiltration of IgG4+ plasma cells in symptomatic patients with ileal pouch-anal anastomosis
    J Crohns Colitis. 5: 570-6.
    9. View The Latest Product References
  9. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders.
    J Clin Pathol. 64 (3): 237-43.
  10. Shiokawa, M. et al. (2013) Risk of cancer in patients with autoimmune pancreatitis.
    Am J Gastroenterol. 108 (4): 610-7.
  11. Whelan, S.F. et al. (2013) Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
    Blood. 121: 1039-48.
  12. Fujimoto, M. et al. (2013) Stromal plasma cells expressing immunoglobulin G4 subclass in non-small cell lung cancer.
    Hum Pathol. 44 (8): 1569-76.
  13. Engelmann, R. et al. (2015) Bone resorption correlates with the frequency of CD5⁺ B cells in the blood of patients with rheumatoid arthritis.
    Rheumatology (Oxford). 54 (3): 545-53.
  14. Ráty, S. et al. (2015) Tumor-like Chronic Pancreatitis Is Often Autoimmune Pancreatitis.
    Anticancer Res. 35 (11): 6163-6.
  15. Agaimy, A. et al. (2015) SMARCA4-deficient undifferentiated carcinoma of the ovary (small cell carcinoma, hypercalcemic type): clinicopathologic and immunohistochemical study of 3 cases.
    Ann Diagn Pathol. 19 (5): 283-7.
  16. Pan, Q. et al. (2016) Association between IgG4 Autoantibody and Complement Abnormalities in Systemic Lupus Erythematosus.
    Mediators Inflamm. 2016: 2196986.
  17. Riedemann, N.C. et al. (2017) Controlling the anaphylatoxin C5a in diseases requires a specifically targeted inhibition.
    Clin Immunol. 180: 25-32.
  18. Engelmann, R. et al. (2018) Decreased IgG4 ACPA levels in responders and increased CD1c+ classical dendritic cells in non-responders of patients with rheumatoid arthritis under therapy.
    Clin Rheumatol. 37 (7): 1783-90.
  19. Derakhshandeh, R. et al. (2021) Single Institutional Experience on Orbital Inflammatory Pseudotumor: Diagnostic and Management Challenge.
    Balkan Med J. 38 (4): 239-43.
  20. Agaimy, A. et al. (2023) Paravertebral fibrous pseudotumor: Four cases of a distinctive tumefactive lesion overlapping with eosinophilic angiocentric fibrosis and tumoral erythema elevatum diutinum.
    Ann Diagn Pathol. 62: 152073.
  21. Koneczny, I. et al. (2024) A retrospective multicenter study on clinical and serological parameters in patients with MuSK myasthenia gravis with and without general immunosuppression.
    Front Immunol. 15: 1325171.

ELISA

Immunohistology - Paraffin

RRID
AB_323679
UniProt
P01861
Entrez Gene
IGHG4
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response

MCA2098P

150267 155900 156094 158892 161556

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