Trastuzumab antibody | AbD16712_hIgG1

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Human anti Trastuzumab:HRP

Anti-trastuzumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical assay development. It can be used as a reference standard in an ADA bridging ELISA and as detection antibody in a PK bridging ELISA with HCA169.

Human anti Trastuzumab

Anti-trastuzumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical assay development. It can be used as a reference standard in an ADA bridging ELISA and as detection antibody in a PK bridging ELISA with HCA169.

Product Type
Monoclonal Antibody
Clone
AbD16712_hIgG1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA176P E 0.1 mg
HCA176 E 0.1 mg
Human anti Trastuzumab, clone AbD16712_hIgG1, is an anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody drug trastuzumab, also known as Herceptin®. Clone AbD16712_hIgG can be used to measure the levels of trastuzumab and biosimilar products using bioanalytical assays.

In the HRP-conjugated format clone AbD16712_hIgG1 is ideal for use as a detection antibody in pharmacokinetic (PK) assays. It is a fully human antibody and therefore may also be used as a calibrator in immune response assays to measure the anti-drug-antibody (ADA) response in patient sera.

Trastuzumab is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells

View a summary of all anti-trastuzumab antibodies.

Product Details

Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Trastuzumab (Herceptin®)
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.
Guarantee
12 months from date of despatch.

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Herceptin® is a registered trademark of Genentech, Inc.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Trastuzumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with HCA169 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
ELISA
This product may be used as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human IgG1 Lambda HCA049 0.1 mg E

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Trastuzumab HCA166 0.1 mg E
Human anti Trastuzumab HCA169 0.1 mg E
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Trastuzumab HCA166 0.1 mg E
Human anti Trastuzumab HCA167 0.1 mg E
Human anti Trastuzumab HCA168 0.1 mg E
Human anti Trastuzumab HCA169 0.1 mg E
Human anti Trastuzumab HCA177 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK001P 1 Conjugation For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK003P 1 Conjugation For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK004P 5 Conjugations For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK005P 1 Conjugation For 20mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK006P 3 Conjugations For 40µg Antibody CJ
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P 0.2 mg C* E

Product Specific References

References for Trastuzumab antibody

  1. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    US Patent Application US20170315118
  2. Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
    Anal Biochem. 540-541: 30-7.
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