Trastuzumab antibody | AbD16712_hIgG1

Human anti Trastuzumab

Product Type
Monoclonal Antibody
Clone
AbD16712_hIgG1
Isotype
IgG1
Specificity
Trastuzumab

Product Code Applications Pack Size List Price Your Price Qty
HCA176
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
HCA176G
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SDS Safety Datasheet SDS
E 1 mg loader
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Search for Batch Specific Datasheets

Human anti Trastuzumab, clone AbD16712_hIgG1, is an anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody drug trastuzumab, also known as Herceptin®. Clone AbD16712_hIgG can be used to measure the levels of trastuzumab and biosimilar products using bioanalytical assays.

In the HRP-conjugated format clone AbD16712_hIgG1 is ideal for use as a detection antibody in pharmacokinetic (PK) assays. It is a fully human antibody and therefore may also be used as a calibrator in immune response assays to measure the anti-drug-antibody (ADA) response in patient sera.

Trastuzumab is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells

View a summary of all anti-trastuzumab antibodies.

Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Trastuzumab (Herceptin®)
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml.
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Herceptin® is a registered trademark of Genentech, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human IgG1 Lambda Allotype G1m3 HCA049 E 0.1 mg loader
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Description Recombinant Human IgG1 Lambda Allotype G1m3

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Trastuzumab HCA166 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA167 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA168 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA169 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA177 E 0.1 mg loader
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Description Human anti Trastuzumab
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
LYNX Rapid HRP Antibody Conjugation Kit LNK003P CJ 1 Conjugation For 4mg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
LYNX Rapid HRP Antibody Conjugation Kit LNK004P CJ 5 Conjugations For 4mg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
LYNX Rapid HRP Antibody Conjugation Kit LNK005P CJ 1 Conjugation For 20mg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
LYNX Rapid HRP Antibody Conjugation Kit LNK006P CJ 3 Conjugations For 40µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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Description Mouse anti Human IgG (Fc) CH2 Domain:HRP

References for Trastuzumab antibody

  1. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    US Patent Application US20170315118
  2. Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
    Anal Biochem. 540-541: 30-7.

Synonyms
HERCEPTIN®
RRID
AB_11152942
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"