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CAS9 antibody | AbD34371kg

Human anti CAS9

Product Type
Monoclonal Antibody

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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Human anti Cas9 antibody, clone AbD34371kg recognizes CRISPR-associated endonuclease Cas9, also known as Cas9 and SpCas9.

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), together with CRISPR-associated (cas) genes, form an adaptive immune system found in some prokaryotes including Streptococcus pyogenes. The system allows direct gene editing in response to viral (bacteriophage) threat, allowing the prokaryote to create a sequence-specific memory of the infection and provide immunity (Sternberg et al. 2014). Cas9 facilitates gene editing as an RNA-guided endonuclease and its guide sequence can be easily altered in the lab, reprogramming Cas9 to target new sites (Wang et al. 2016). Importantly, Cas9 can be co-opted for in vitro genome alteration in mammalian cells, allowing production of modified clonal cell lines within 2-3 weeks (Ann Ran et al. 2013). Co-opting the CRISPR-Cas9 system has strong implications for personalized medicine (Xing and Meng 2020), and successful treatment of patients with inherited disease has been achieved using CRISPR (Ribeil et al. 2017). Human anti Cas9 antibody, clone AbD34371kg, has been tested in western blotting. Anti rabbit IgG specific antibodies are recommended as secondary detection reagents.

Target Species
Streptococcus sp.
Product Form
Recombinant chimeric human/rabbit IgG antibody selected from the HuCAL phage display library and expressed in a human cell line. In this antibody, the VH-CH1 regions and light chains are human while the Fc region is derived from rabbit IgG. This antibody is supplied as a liquid.
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Wild type recombinant Cas9 from Streptococcus pyogenes, expressed in E.coli.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/250 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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