Serotonin antibody | YC5/45

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Rat anti Serotonin

Product Type
Monoclonal Antibody
Clone
YC5/45
Isotype
IgG2
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8250-0495 C* IF 0.1 ml
Rat anti serotonin antibody, clone YC5/45 recognizes serotonin and does not cross react with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.

Serotonin, also known as 5-hydroxytryptamine or 5-HT is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotonin is widely distributed in the central and peripheral nervous system, and also in some non-neural tissues such as platelets.

Serotonin appears widely conserved and Rat anti serotonin antibody, clone YC5/45 has been used successfully to demonstrate serotonin immunoreactivity using immunohistochemistry in many diverse groups including primates (Vanoli et al. 2013), rodents (Hosoda et al. 1984), prototherian mammals, avians and reptiles (Adamson and Campbell 1988), teleosts (Takeda et al. 2014) through to invertebrates including hirudinids (Masuda-Nakagawa et al. 1990) and gastropods (Osborne and Dockray 1982).

Product Details

Target Species
Broad
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Goldfish
Cat
Chicken
Marine file snake
Helix aspersa
Hirudo medicinalis
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.05%Thiomersal
Immunogen
Serotonin conjugated to bovine serum albumin.
Fusion Partners
Spleen cells from immunised COB wistar rats were fused with cells of the Y3-Ag-1.2.3 rat myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Serotonin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1
  1. 1Formaldehyde fixation is recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for Serotonin antibody

  1. Consolazione, A. et al. (1981) Immunocytochemical detection of serotonin with monoclonal antibodies.
    J Histochem Cytochem. 29: 1425-30.
  2. Milstein, C. et al. (1983) The discrepancy between the cross-reactivity of a monoclonal antibody to serotonin and its immunohistochemical specificity.
    Mol Immunol. 20 (1): 113-23.
  3. Suárez-Quian, C.A. (1987) The distribution of four lysosomal integral membrane proteins (LIMPs) in rat basophilic leukemia cells.
    Tissue Cell. 19: 495-504.
  4. La Rosa, S. et al. (2011) Histologic characterization and improved prognostic evaluation of 209 gastric neuroendocrine neoplasms.
    Hum Pathol. 42: 1373-84.
  5. Takeda, A. et al. (2015) Axonal regeneration through the fibrous scar in lesioned goldfish spinal cord.
    Neuroscience. 284: 134-52.
  6. Cuello, A.C. et al. (1982) Immunocytochemistry with internally labeled monoclonal antibodies.
    Proc Natl Acad Sci U S A. 79: 665-9.
  7. Gil-Loyzaga, P. et al. (1997) Serotonergic innervation of the organ of Corti of the cat cochlea.
    Neuroreport. 8: 3519-22.
  8. Osborne, N.N. (1982) Uptake, localization and release of serotonin in the chick retina.
    J Physiol. 331: 469-79.
  9. Vanoli, A. et al. (2013) Histologic changes in type A chronic atrophic gastritis indicating increased risk of neuroendocrine tumor development: the predictive role of dysplastic and severely hyperplastic enterochromaffin-like cell lesions.
    Hum Pathol. 44: 1827-37.
  10. Dainese, E. et al. (2013) Linear and micronodular neuroendocrine cell hyperplasia in an ovarian mucinous cystadenoma.
    Pathol Res Pract. 209: 670-3.
  11. Solcia, E. et al. (1990) Gastric argyrophil carcinoidosis in patients with Zollinger-Ellison syndrome due to type 1 multiple endocrine neoplasia. A newly recognized association.
    Am J Surg Pathol. 14 (6): 503-13.