IgG2c antibody | MARG2c-5

Mouse anti Rat IgG2c Heavy Chain

Product Type
Monoclonal Antibody
Clone
MARG2c-5
Isotype
IgG2a
Specificity
IgG2c
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA1295
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F 0.5 mg loader
List Price Your Price
loader

Mouse anti Rat IgG2c heavy chain antibody, clone MARG2c-5 recognizes the heavy chain of rat immunoglobulin gamma 2C as determined by dot blot analysis.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
IgG2c (Rat strain LOU/c).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml as coating antibody
Flow Cytometry 1/200 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
ELISA
Clone MARG2c-5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with (HRP-conjugated MARK-1/MARL-15 clone cocktail, MCA1296P) as the detection reagent

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG2a Negative Control

References for IgG2c antibody

  1. Manouvriez, P.et al. (1986) Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by ELISA with mouse monoclonal antibodies.
    Immunobiology 173: 307-8.
  2. Delriviere, L.D. et al. (1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.
    Transplantation. 66: 112-5.
  3. Lin, Y. et al. (1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.
    J Immunol. 163: 2850-7.
  4. Lin, Y. et al. (2000) Long-term survival of hamster hearts in presensitized rats.
    J Immunol. 164: 4883-92.
  5. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.

Further Reading

  1. van den Berg, T.K. et al. (2001) Monoclonal antibodies against rat leukocyte surface antigens.
    Immunol Rev. 184: 109-16.

RRID
AB_321841
UniProt
P20762
Entrez Gene
LOC362795
GO Terms
GO:0003823 antigen binding

MCA1295

180220

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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