IgG2c antibody | MARG2c-5
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Mouse anti Rat IgG2c Heavy Chain:FITC
- Product Type
- Monoclonal Antibody
- Clone
- MARG2c-5
- Isotype
- IgG2a
- Specificity
- IgG2c
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti Rat IgG2c heavy chain antibody, clone MARG2c-5 recognizes the heavy chain of rat immunoglobulin gamma 2C as determined by dot blot analysis. |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- IgG2c (Rat strain LOU/c).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control:FITC | ||||||
References for IgG2c antibody
-
Delriviere, L.D. et al. (1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.
Transplantation. 66: 112-5. -
Lin, Y. et al. (1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.
J Immunol. 163: 2850-7. -
Lin, Y. et al. (2000) Long-term survival of hamster hearts in presensitized rats.
J Immunol. 164: 4883-92. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67.
Further Reading
-
van den Berg, T.K. et al. (2001) Monoclonal antibodies against rat leukocyte surface antigens.
Immunol Rev. 184: 109-16.
- RRID
- AB_323212
- UniProt
- P20762
- Entrez Gene
- LOC362795
- GO Terms
- GO:0003823 antigen binding
MCA1295F
154652
164601
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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