IgG2c antibody | MARG2c-5

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			5ug/ml as coating antibody
Flow Cytometry		1/200	1/1000

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

ELISA

Clone MARG2c-5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with (HRP-conjugated MARK-1/MARL-15 clone cocktail, MCA1296P) as the detection reagent

References for IgG2c antibody

1. Manouvriez, P.et al. (1986) Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by ELISA with mouse monoclonal antibodies.
   Immunobiology 173: 307-8.
2. Delriviere, L.D. et al. (1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.
   Transplantation. 66: 112-5.
3. Lin, Y. et al. (1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.
   J Immunol. 163: 2850-7.
4. Lin, Y. et al. (2000) Long-term survival of hamster hearts in presensitized rats.
   J Immunol. 164: 4883-92.
5. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
   Int Immunol. 30 (2): 53-67.

Further Reading

1. van den Berg, T.K. et al. (2001) Monoclonal antibodies against rat leukocyte surface antigens.
   Immunol Rev. 184: 109-16.

RRID

AB_321841

UniProt

P20762

Entrez Gene

LOC362795

GO Terms

GO:0003823 antigen binding

MCA1295

180220

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