IgG2c antibody | MARG2c-5
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Mouse anti Rat IgG2c heavy chain antibody, clone MARG2c-5 recognizes the heavy chain of rat immunoglobulin gamma 2C as determined by dot blot analysis.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- IgG2c (Rat strain LOU/c).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P20762 Related reagents
- Entrez Gene
- LOC362795 Related reagents
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG2c antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 5ug/ml | ||
| ELISA | 5ug/ml as coating antibody | ||
| Flow Cytometry | 1/200 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- ELISA
- Clone MARG2c-5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our HRP-conjugated MARK-1/MARL-15 clone cocktail (MCA1296P) as the detection reagent.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
|
|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Product Specific References
References for IgG2c antibody
-
Delriviere, L.D. et al. (1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.
Transplantation. 66: 112-5. -
Lin, Y. et al. (1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.
J Immunol. 163: 2850-7. -
Lin, Y. et al. (2000) Long-term survival of hamster hearts in presensitized rats.
J Immunol. 164: 4883-92. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67.
Further Reading
-
van den Berg, T.K. et al. (2001) Monoclonal antibodies against rat leukocyte surface antigens.
Immunol Rev. 184: 109-16.
