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CD31 antibody | TLD-3A12

Mouse anti Rat CD31:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 50 µg loader
List Price Your Price
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.

Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009).

Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
MCA1334FB, MCA1334F: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1334FA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Activated, Lewis rat derived microglial cells.
Approx. Protein Concentrations
MCA1334FB: IgG concentration 0.5 mg/ml
MCA1334FA, MCA1334F: IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100 Pack Size: 0.5 mg
Neat Pack Size: 0.1 mg
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. Flaris, N.A. et al. (1993) Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction.
    Glia. 7 (1): 34-40.

References for CD31 antibody

  1. Williams, K.C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat.
    J Neurosci Res. 45 (6): 747-57.
  2. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration.
    J Pharmacol Exp Ther. 286: 447-58.
  3. MacLean, A.G. et al. (2001) Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells.
    J Neuroimmunol. 118: 223-32.
  4. Frye, C.A. & Patrick, C.W. Jr (2002) Isolation and culture of rat microvascular endothelial cells.
    In Vitro Cell Dev Biol Anim. 38 (4): 208-12.
  5. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury.
    Am J Pathol. 163: 1587-98.
  6. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation.
    J Pharmacol Exp Ther. 306: 8-12.
  7. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis.
    Arthritis Rheum. 48: 2173-7.
  8. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues.
    J Immunol Methods. 322: 137-42.
  9. View The Latest Product References
  10. Ohnishi, T. et al. (2007) Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans.
    Toxicol Pathol. 35: 904-9.
  11. Ceelen, W. et al. (2007) Recombinant human erythropoietin alpha modulates the effects of radiotherapy on colorectal cancer microvessels.
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  12. Fujimoto, K.L. et al. (2007) An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction.
    J Am Coll Cardiol. 49: 2292-300.
  13. Arkudas, A. et al. (2007) Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model.
    Mol Med. 13: 480-7.
  14. Schilte, M.N. et al. (2009) Long-term intervention with heparins in a rat model of peritoneal dialysis.
    Perit Dial Int. 29: 26-35.
  15. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18 (10): 1389-98.
  16. Pedram, M.S. et al. (2010) Transplantation of a combination of autologous neural differentiated and undifferentiated mesenchymal stem cells into injured spinal cord of rats.
    Spinal Cord. 48 (6): 457-63.
  17. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development.
    Am J Pathol. 157: 647-58.
  18. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation.
    J Immunol. 183: 3099-108.
  19. Willis, C.L. et al. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation.
    J Cereb Blood Flow Metab. 30: 1847-59.
  20. Lochhead, J.J. et al. (2010) Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation.
    J Cereb Blood Flow Metab. 30: 1625-36.
  21. Teng, B.T. et al. (2011) Protective effect of caspase inhibition on compression-induced muscle damage.
    J Physiol. 589: 3349-69.
  22. Salehi-Had, H. et al. (2011) Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization.
    PLoS One. 6: e18864.
  23. Nakao, A. et al. (2011) Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts.
    Cardiovasc Res. 89: 457-63.
  24. Sheu, J.J. et al. (2012) Combination of cilostazol and clopidogrel attenuates rat critical limb ischemia.
    J Transl Med. 10: 164.
  25. Matsugami, al. (2014) VEGF secretion by adipose tissue-derived regenerative cells is impaired under hyperglycemic conditions via glucose transporter activation and ROS increase.
    Biomed Res. 35 (6): 397-405.
  26. Brandl, A. et al. (2014) A novel early precursor cell population from rat bone marrow promotes angiogenesis in vitro.
    BMC Cell Biol. 15: 12.
  27. Oboshi, M. et al. (2015) Temporary dietary iron restriction affects the process of thrombus resolution in a rat model of deep vein thrombosis.
    PLoS One. 10 (5): e0126611.
  28. Stavenuiter, A.W. et al. (2015) Protective Effects of Paricalcitol on Peritoneal Remodeling during Peritoneal Dialysis.
    Biomed Res Int. 2015: 468574.
  29. Naaijkens BA et al. (2015) Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue.
    Cell Tissue Res. 362 (3): 623-32.
  30. Sun, C.K. et al. (2015) Mixed serum-deprived and normal adipose-derived mesenchymal stem cells against acute lung ischemia-reperfusion injury in rats.
    Am J Transl Res. 7 (2): 209-31.
  31. Kakaiy, A. et al. (2015) Comparing protective effect of grape seed extract versus atorvastatin on endometriosis in rat model: Evidence for immunohistochemical and biochemical alterations.
    Vet Res Forum. 6 (2): 101-10.
  32. Ikutomi, M. et al. (2015) Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation.
    J Mol Cell Cardiol. 86: 121-35.
  33. Wu, S.H. et al. (2015) Autologous adipose-derived stem cells attenuate muscular atrophy and protect spinal cord ventral horn motor neurons in an animal model of burn injury.
    Cytotherapy. 17 (8): 1066-75.
  34. Jiang, Y. et al. (2015) SOD1 nanozyme salvages ischemic brain by locally protecting cerebral vasculature.
    J Control Release. 213: 36-44.
  35. Tung, H.C. et al. (2015) The Beneficial Effects of P2X7 Antagonism in Rats with Bile Duct Ligation-induced Cirrhosis.
    PLoS One. 10 (5): e0124654.
  36. Park; J.R. et al. (2016) Effects of Peroxisome Proliferator-Activated Receptor-δ Agonist on Cardiac Healing after Myocardial Infarction.
    PLoS One. 11 (2): e0148510.
  37. Lux, M. et al. (2016) In vitro maturation of large-scale cardiac patches based on a perfusable starter matrix by cyclic mechanical stimulation.
    Acta Biomater. 30: 177-87.
  38. Ferrantelli, E. et al. (2016) The dipeptide alanyl-glutamine ameliorates peritoneal fibrosis and attenuates IL-17 dependent pathways during peritoneal dialysis.
    Kidney Int. 89 (3): 625-35.
  39. Mirzaei, M. et al. (2017) Nanosilver particles increase follicular atresia: Correlation with oxidative stress and aromatization.
    Environ Toxicol. 32 (10): 2244-55.
  40. Lim, S. et al. (2017) Attenuation of carotid neointimal formation after direct delivery of a recombinant adenovirus expressing glucagon-like peptide-1 in diabetic rats.
    Cardiovasc Res. 113 (2): 183-94.
  41. Ichihara, Y. et al. (2018) Self-assembling peptide hydrogel enables instant epicardial coating of the heart with mesenchymal stromal cells for the treatment of heart failure.
    Biomaterials. 154: 12-23.
  42. Melly, L. et al. (2018) Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels.
    J Cell Mol Med. 22 (5): 2580-91.
  43. Costa, B.P. et al. (2018) Intestinal Epithelial Stem Cells: Distinct Behavior After Surgical Injury and Teduglutide Administration.
    J Invest Surg. 31 (3): 243-52.
  44. Sønstevold, T. et al. (2018) Hyperbaric oxygen treatment did not significantly affect radiation injury in the mandibular area of rats.
    Oral Surg Oral Med Oral Pathol Oral Radiol. 125 (2): 112-9.
  45. Aminzadeh, A. et al. (2020) Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
    Cell J. 22 (Suppl 1): 38-48.
  46. Kuriyama, T. et al. (2020) A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer.
    Regen Ther. 14: 1-10.
  47. Pun, C.K. et al. (2021) Glycyrrhizin Attenuates Portal Hypertension and Collateral Shunting via Inhibition of Extrahepatic Angiogenesis in Cirrhotic Rats.
    Int J Mol Sci. 22(14):7662.
  48. Huang, H.C. et al. (2021) Matrix metalloproteinase-9 inhibition or deletion attenuates portal hypertension in rodents.
    J Cell Mol Med. 25 (21): 10073-87.
  49. Huang, H.C. et al. (2021) Microbiota transplants from feces or gut content attenuated portal hypertension and portosystemic collaterals in cirrhotic rats.
    Clin Sci (Lond). 135 (24): 2709-2728.
  50. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.
  51. Huang, H.C. et al. (2022) Effects of PCSK-9 Inhibition by Alirocumab Treatments on Biliary Cirrhotic Rats.
    Int J Mol Sci. 23 (13): 7378.
  52. Zhou, X. et al. (2022) Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.
    Nat Commun. 13 (1): 6672.
  53. Sun, Y. et al. (2023) Effects of acupuncture on angiogenesis-associated factor expression in ischemic brain tissue following cerebral infarction in rats
    Acupuncture and Herbal Medicine. Jan [Epub ahead of print].
  54. Zhang, Q. et al. (2023) Harnessing the synergy of perfusable muscle flap matrix and adipose-derived stem cells for prevascularization and macrophage polarization to reconstruct volumetric muscle loss.
    Bioact Mater. 22: 588-614.
  55. Pun, C.K. et al. (2023) Dual angiotensin receptor and neprilysin inhibitor reduced portal pressure through peripheral vasodilatation and decreasing systemic arterial pressure in cirrhotic rats.
    J Chin Med Assoc. 86 (9): 786-94.
  56. Wu, H.N. et al. (2023) Molecular Mechanism of Angiogenesis for Cerebral Infarction Rats by Acupuncture Intervention Based on Sonic Hedgehog Signaling Pathway.
    Physiol Behav. Nov 28:114420 [Epub ahead of print].
  57. Hsin, I.F. et al. (2018) Insulin reverses major portal hypertension-related derangements in rats with liver cirrhosis and diabetes.
    Clin Sci (Lond). 132 (22): 2391-405.
  58. Wang, X. et al. (2024) Curcumol Attenuates Portal Hypertension and Collateral Shunting Via Inhibition of Extrahepatic Angiogenesis in Cirrhotic Rats.
    Biochem Genet. Mar 04 [Epub ahead of print].
  59. Huang, H.C. et al. (2022) Lycopene treatment improves intrahepatic fibrosis and attenuates pathological angiogenesis in biliary cirrhotic rats.
    J Chin Med Assoc. 85 (4): 414-20.

Flow Cytometry


Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0002687 positive regulation of leukocyte migration
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006928 cellular component movement
GO:0009986 cell surface
GO:0030054 cell junction
GO:0034097 response to cytokine stimulus


0913 163512




152267 159444

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