Ranibizumab antibody | AbD16746_hIgG1
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human Anti-Ranibizumab Antibody, clone AbD16746_hIgG1 (HCA305) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with recombinant human VEGF-A (PHP293), ranibizumab, bevacizumab or human Fab VH3-23/kappa1 at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, the human VEGF-A/ranibizumab complex was formed by adding 2 µg/ml ranibizumab to the wells coated with VEGF-A. Detection was performed using HRP conjugated Human Anti-Ranibizumab Antibody, clone AbD16746_hIgG1 (HCA305) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with ranibizumab at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human Anti-Ranibizumab Antibody, clone AbD16746_hIgG1 (HCA305). Detection was performed using HRP conjugated ranibizumab followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the ranibizumab used in this assay was performed using a LYNX Rapid HRP Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
Filter by Application:
E ResetHuman anti Ranibizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD16746_hIgG1
- Isotype
- IgG1
- Specificity
- Ranibizumab
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| Human Anti-Ranibizumab Antibody, clone AbD16746_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody in IgG1 format that recognizes the monoclonal antibody drug ranibizumab. The antibody does not recognize recombinant human vascular endothelial growth factor A (VEGF-A) or ranibizumab when in complex with recombinant human VEGF-A. Clone AbD16746_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a control or calibration standard in an anti-drug antibody (ADA) assay. This antibody also recognizes the monoclonal antibody drug bevacizumab. Ranibizumab (Lucentis) is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab (Avastin); it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab. Ranibizumab was first approved to treat wet age-related macular degeneration (AMD), a common form of age-related vision loss. It is also approved for the treatment of macular edema following retinal vein occlusion, diabetic macular edema, and diabetic retinopathy. Ranibizumab inhibits angiogenesis by binding to VEGF-A, blocking its interaction with VEGF receptors on the surface of endothelial cells, and thus preventing the subsequent growth of new blood vessels. View a summary of all anti-ranibizumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Ranibizumab
- Affinity
- The monovalent intrinsic affinity of AbD16746_hIgG1 was measured as KD= 2 nM by real time, label free molecular interaction analysis on immobilized bevacizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Lucentis is a trademark of Genentech.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD16746_hIgG1 can be used in a direct or indirect ELISA or as a calibrator for the set-up of an anti-drug antibody assay.
Protocol: ADA bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
- Synonyms
- Lucentis
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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