CD52 antibody | 11/305/44
Mouse anti Pig CD52, clone 11/305/44 is a monoclonal antibody recognizing the porcine homologue of human CD52, a ~19 kDa antigen expressed by mature lymphocytes, monocytes and dendritic cells.
Mouse anti Pig CD52, clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1 (Lunney et al.1994). SWC1 is the porcine orthologue to human CD52, expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets (Piriou-Guyzlack et al. 2008) & (Leitner et al. 2012). Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999) & (McCollough et al. 1999).
Mouse anti Pig CD52, clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1 (Lunney et al.1994). SWC1 is the porcine orthologue to human CD52, expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets (Piriou-Guyzlack et al. 2008) & (Leitner et al. 2012). Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999) & (McCollough et al. 1999).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- H8ZRT6
- Regulatory
- For research purposes only
Applications of CD52 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | STAR117D405GA | F IF | 0.1 mg |
|
|
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg |
|
|
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests |
|
|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig CD203a | MCA1973GA | C F IP | 0.1 mg |
|
|
| Mouse anti Pig CD203a:FITC | MCA1973F | F | 0.1 mg |
|
Product Specific References
References for CD52 antibody
-
Leitner, J. et al. (2012) Porcine SWC1 is CD52--final determination by the use of a retroviral cDNA expression library.
Vet Immunol Immunopathol. 146 (1): 27-34. -
Lunney, J.K. et al. (1994) Overview of the First International Workshop to Define Swine Leukocyte Cluster of Differentiation (CD) Antigens.
Vet Immunol Immunopathol. 43: 193-206. -
Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 43: 35. -
Shao, L. et al. (2016) Tissue-specific mRNA expression profiles of porcine Toll-like receptors at different ages in germ-free and conventional pigs.
Vet Immunol Immunopathol. 171: 7-16.
Further Reading
-
Piriou-Guzylack, L. et al. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54 -
Sánchez, C. et al. (1999) The Porcine 2A10 Antigen is Homologous to Human CD163 and Related to Macrophage Differentiation.
J Immunol. 162: 5230-7. -
Mccullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
Immunology. 98 (2): 203-12.
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