CD203a antibody | PM18-7

Mouse anti Pig CD203a:FITC

Product Type
Monoclonal Antibody
Clone
PM18-7
Isotype
IgG1
Specificity
CD203a

Product Code Applications Pack Size List Price Your Price Qty
MCA1973F
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SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007).

Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of porcine alveolar macrophages.

CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015).

SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Porcine alveolar macrophages.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with P3X63-Ag8-653 murine myeloma cells (Kearney et al. 1979).
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

References for CD203a antibody

  1. McCullough, K.C. et al. (1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.
    Vet Immunol Immunopathol. 58 (3-4): 265-75.
  2. McCullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
    Immunology. 98 (2): 203-12.
  3. Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
    Vet Immunol Immunopathol. 80 (1-2): 63-78.
  4. Domínguez, J. et al. (1998) Porcine myelomonocytic markers: summary of the Second International Swine CD Workshop.
    Vet Immunol Immunopathol. 60 (3-4): 329-41.
  5. Dominguez, J. et al. (1998) Workshop studies with monoclonal antibodies identifying a novel porcine differentiation antigen, SWC9.
    Vet Immunol Immunopathol. 60 (3-4): 343-9.
  6. Petersen, C.B. et al. (2007) Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies.
    Dev Comp Immunol. 31 (6): 618-31.
  7. Basta, S. et al. (1999) Modulation of monocytic cell activity and virus susceptibility during differentiation into macrophages.
    J Immunol. 162 (7): 3961-9.
  8. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
    Vet Res. 42: 9.
  9. View The Latest Product References
  10. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
    J Immunol. 162 (9): 5230-7.
  11. Cantu, E. et al. (2006) Depletion of pulmonary intravascular macrophages prevents hyperacute pulmonary xenograft dysfunction.
    Transplantation. 81 (8): 1157-64.
  12. Basta, S. et al. (2001) Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility.
    Immunology. 103 (4): 488-97.
  13. Lithgow, P. et al. (2014) Correlation of cell surface marker expression with African swine fever virus infection.
    Vet Microbiol. 168: 413 - 9.
  14. Ondrackova, P. et al. (2013) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and< i> Salmonella-infected piglets by seven-colour flow cytometry.
    Res Vet Sci. 94: 240 - 5.
  15. Tsai, Y.C. et al. (2014) Differences in the expression of innate immune response-modulating genes in blood monocytes between subclinically porcine circovirus type s (PCV2)-infected and PCV2-free pigs prior to and after lipopolysaccharide stimulation in vitro
    Taiwan Veterinary Journal. 40 (01): 37-48.
  16. Hwang, J.H.et al. (2015) Characterization of monoclonal antibodies against porcine pulmonary alveolar macrophages of gnotobiotic miniature swine.
    Biochem Biophys Res Commun. 461 (2): 427-34.
  17. Shao, L. et al. (2016) Tissue-specific mRNA expression profiles of porcine Toll-like receptors at different ages in germ-free and conventional pigs.
    Vet Immunol Immunopathol. 171: 7-16.
  18. Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
    Dev Comp Immunol. 81: 95-104.
  19. Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
    Dev Comp Immunol. 84: 181-92.
  20. Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
    PLoS Pathog. 13 (2): e1006206.
  21. Zimmermann, C.E. et al. (2021) Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.
    J Stem Cells Regen Med. 17 (2): 49-55.

Further Reading

  1. Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39 (6): 54.

Flow Cytometry

Synonyms
SWC9
RRID
AB_324137
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