Mouse anti Pig CD45RC, clone MIL5
recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the ~210 kDa RC and ~226 kDa RAC isoforms. MIL15, binds to an epitope alternative to that bound by clone MIL5
, also against CD45RC differing in their ability to stain B cells in tissue sections (Zuckerman et al. 2001
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- Cells isolated from porcine mesenteric lymph node.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RC antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD45RC antibody
Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
Vet Immunol Immunopathol. 80 (1-2): 165-74.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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