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CD45RC antibody | MIL15

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MCA1750
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SDS Safety Datasheet SDS
C F P 2 ml loader
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loader

Mouse anti Pig CD45RC, clone MIL5 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the ~210 kDa RC and ~226 kDa RAC isoforms. MIL15, binds to an epitope alternative to that bound by clone MIL5, also against CD45RC differing in their ability to stain B cells in tissue sections (Zuckerman et al. 2001).

Target Species
Pig
Product Form
Tissue culture supernatant - liquid
Preparation
Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Cells isolated from porcine mesenteric lymph node.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for CD45RC antibody

  1. Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
    Vet Immunol Immunopathol. 80 (1-2): 165-74.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

RRID
AB_2174250

MCA1750

155871 L1512

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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