CD45RC antibody | MIL15
Mouse anti Pig CD45RC
- Product Type
- Monoclonal Antibody
- Clone
- MIL15
- Isotype
- IgM
- Specificity
- CD45RC
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD45RC, clone MIL15 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the ~210 kDa RC and ~226 kDa RAC isoforms. MIL15, binds to an epitope alternative to that bound by clone MIL5, also against CD45RC differing in their ability to stain B cells in tissue sections (Zuckerman et al. 2001). |
- Target Species
- Pig
- Product Form
- Tissue culture supernatant - liquid
- Preparation
- Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Cells isolated from porcine mesenteric lymph node.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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References for CD45RC antibody
-
Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
Vet Immunol Immunopathol. 80 (1-2): 165-74.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- RRID
- AB_2174250
MCA1750
155871
L1512
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD45RC specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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