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CD335 antibody | VIV-KM1

Mouse anti Pig CD335:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
VIV-KM1
Isotype
IgG1
Specificity
CD335

Product Code Applications Pack Size List Price Your Price Qty
MCA5972A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Mouse anti Pig CD335 antibody, clone VIV-KM1 recognizes the porcine homologue of human CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family.

CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development. CD335 is expressed by human natural killer cells (Sivori et al.1997). The development of monoclonal antibodies to bovine CD335 (clone AKS1) (Storset et al. 2004) and ovine CD335 (clone EC1.1) (Connelley et al.2011) have enabled researchers to identify and better understand ruminant NK cells.

Mouse anti Pig CD335 antibody, clone VIV-KM is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the pig immune system and aid in the understanding of the role of NK cells in host pathogen defense. Porcine CD335 is not expressed by all NK cells and expression may be influenced by cytokine production (Mair et al. 2012).

Target Species
Pig
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Fusion protein consisting of the extracellular region of porcine CD335.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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Description Mouse IgG1 Negative Control:Alexa Fluor® 488

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Source Reference

  1. Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
    Eur J Immunol. 42 (5): 1261-71.

References for CD335 antibody

  1. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  2. Forberg, H. et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
    PLoS One. 9 (6): e100619.
  3. Yang, G. et al. (2017) Characterizing porcine invariant natural killer T cells: A comparative study with NK cells and T cells.
    Dev Comp Immunol. 76: 343-51.
  4. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  5. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.
  6. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.

Further Reading

  1. Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation.
    J Exp Med. 186 (7): 1129-36.
  2. Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.
    Eur J Immunol. 34 (3): 669-76.
  3. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.

Synonyms
NKp46

MCA5972A488

152841 1609 162590 168037

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