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Pertuzumab antibody | AbD35668

Human anti Pertuzumab

Product Type
Monoclonal Antibody
HuCAL Fab monovalent

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg
List Price Your Price

Human Anti-Pertuzumab Antibody, clone AbD35668 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody pertuzumab. It does not recognize free human epidermal growth factor receptor 2 (HER2) nor pertuzumab in complex with HER2. The antibody can be used to measure pertuzumab or its biosimilars in patient samples.

Clone AbD35668 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as the capture antibody in a pharmacokinetic (PK) bridging ELISA.

Pertuzumab (Perjeta) is an IgG1/kappa antibody humanized from mouse. It has been approved for the treatment of patients with metastatic HER2-positive breast cancer in combination with trastuzumab. Pertuzumab binds to the extracellular domain II of HER2 and inhibits HER2–HER3 dimerization, reducing signaling through intracellular pathways (Capelan et al. 2013).

View a summary of all anti-pertuzumab antibodies

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK). This antibody is supplied as a liquid.
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Theintrinsic affinity of the monovalent form of Human anti Pertuzumab antibody, clone AbD35668 is KD = 14 nM as measured by real time, label free molecular interaction analysis on immobilized Pertuzumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Perjeta is a trademark of Genentech, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Human anti Pertuzumab antibody, clone AbD35668 can be used in an indirect ELISA or as capture antibody for Pertuzumab in a bridging ELISA.

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Further Reading

  1. Capelan, M. et al. (2013) Pertuzumab: new hope for patients with HER2-positive breast cancer.
    Ann Oncol. 24 (2): 273-282.

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.


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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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