TCR Gamma/Delta antibody | GL3

Hamster anti Mouse TCR Gamma/Delta

Product Type
Monoclonal Antibody
Clone
GL3
Isotype
IgG
Specificity
TCR Gamma/Delta

Product Code Applications Pack Size List Price Your Price Qty
MCA1366GA
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SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 reacts with mouse TCR gamma/delta expressing lymphocytes.

Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 has been shown to have depleting activity in vivo (Van der Heyde et al. 1995 and  Skeenet al. 1993).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
C57BL/6J intra epithelial lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamster were fused with cells of the murine myeloma SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

References for TCR Gamma/Delta antibody

  1. Kinoshita, N. et al. (2002) Autocrine IL-15 mediates intestinal epithelial cell death via the activation of neighboring intraepithelial NK cells.
    J Immunol. 169: 6187-92.
  2. Skelsey, M.E. et al. (2001) Gamma delta T cells are needed for ocular immune privilege and corneal graft survival.
    J Immunol. 166: 4327-33.
  3. Skarstein, K. et al. (1994) Oligoclonality of T cells in salivary glands of autoimmune MRL/lpr mice.
    Immunology. 81 (4): 497-501.
  4. van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
    J Immunol. 154 (8): 3985-90.
  5. Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
    J Exp Med. 178 (3): 971-84.
  6. Karasova, D. et al. (2010) Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis.
    BMC Microbiol. 10: 75.
  7. Laky, K. et al. (2000) Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.
    J Exp Med. 191: 1569-80.

Flow Cytometry

Immunohistology - Frozen

RRID
AB_1102884
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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