TCR Gamma/Delta antibody | GL3
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Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 reacts with mouse TCR gamma/delta expressing lymphocytes.
Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 has been shown to have depleting activity in vivo (Van der Heyde et al. 1995 and Skeenet al. 1993).
Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 has been shown to have depleting activity in vivo (Van der Heyde et al. 1995 and Skeenet al. 1993).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.5% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- C57BL/6J intra epithelial lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized Armenian hamster were fused with cells of the murine myeloma SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
- Guarantee
- 6 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of TCR Gamma/Delta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/5 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:DyLight®549 | STAR104D549GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:DyLight®649 | STAR104D649GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | 0.1 mg | F IF WB | ||
| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | 0.4 mg | F | ||
| Goat anti Hamster IgG:Biotin | STAR79B | 0.8 mg | E F | ||
| Goat anti Hamster IgG:FITC | STAR79F | 0.7 mg | F | ||
| Goat anti Hamster IgG:HRP | STAR79P | 0.8 mg | E |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | 0.1 mg | F | ||
| Hamster (Armenian) IgG Negative Control | MCA2356 | 0.25 mg | F | ||
| Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | 0.1 mg | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Product Specific References
References for TCR Gamma/Delta antibody
-
Skarstein, K. et al. (1994) Oligoclonality of T cells in salivary glands of autoimmune MRL/lpr mice.
Immunology. 81 (4): 497-501. -
van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
J Immunol. 154 (8): 3985-90. -
Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
J Exp Med. 178 (3): 971-84. -
Karasova, D. et al. (2010) Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis.
BMC Microbiol. 10: 75. -
Kinoshita, N. et al. (2002) Autocrine IL-15 mediates intestinal epithelial cell death via the activation of neighboring intraepithelial NK cells.
J Immunol. 169: 6187-92. -
Laky, K. et al. (2000) Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.
J Exp Med. 191: 1569-80. -
Skelsey, M.E. et al. (2001) Gamma delta T cells are needed for ocular immune privilege and corneal graft survival.
J Immunol. 166: 4327-33.
