TCR Alpha/Beta antibody | H57-597

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Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 thumbnail image 1

Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 thumbnail image 2

Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 gallery image 1 — Staining of mouse spleen cells with Hamster anti Mouse TCR alpha/beta (**MCA1413**).

Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 gallery image 2 — Staining of mouse spleen cells with Hamster anti Mouse TCR Alpha/Beta:FITC (**MCA1413F**).

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Hamster anti Mouse TCR Alpha/Beta Beta Chain

Product Type: Monoclonal Antibody
Clone: H57-597
Isotype: IgG
Specificity: TCR Alpha/Beta
Region: BETA CHAIN

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Secondary Antibodies
References (7)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA1413 Datasheet Datasheet SDS SDS	F IP	0.25 mg		Log in	Get Quote
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Hamster anti mouse TCR α/β, clone H57-597, recognizes the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne 1990).

Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.

Target Species: Mouse
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
Carrier Free: Yes
Immunogen: Affinity purified murine T cell receptor.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			0.2-1ug/10⁶ cells
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description	Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC	STAR104F	F	0.4 mg	Log in	Get Quote
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Description	Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin	STAR79B	E F	0.8 mg	Log in	Get Quote
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Description	Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC	STAR79F	F	0.7 mg	Log in	Get Quote
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Description	Goat anti Hamster IgG:FITC
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Description	Goat anti Hamster IgG:HRP
Goat F(ab')2 anti Hamster IgG:DyLight®550	STAR104D550	F IF WB	0.1 mg	Log in	Get Quote
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Description	Goat F(ab')2 anti Hamster IgG:DyLight®550
Goat F(ab')2 anti Hamster IgG:DyLight®650	STAR104D650	F IF	0.1 mg	Log in	Get Quote
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Description	Goat F(ab')2 anti Hamster IgG:DyLight®650

Description	Product Code	Applications	Pack Size	Your Price
Hamster (Armenian) IgG Negative Control	MCA2356	F	0.25 mg	Log in	Get Quote
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Description	Hamster (Armenian) IgG Negative Control

References for TCR Alpha/Beta antibody

Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
J Immunol. 142 (8): 2736-42.
Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
J Exp Med. 170 (5): 1569-81.
Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
J Biol Chem. 265 (16): 9296-301.
van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
J Immunol. 154 (8): 3985-90.
Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
J Exp Med. 178 (3): 971-84.
Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
Glia. 59 (1): 45-57.
Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
PLoS One. 7 (7): e40657.