TCR Alpha/Beta antibody | H57-597

Hamster anti Mouse TCR Alpha/Beta Beta Chain

Product Type
Monoclonal Antibody
Clone
H57-597
Isotype
IgG
Specificity
TCR Alpha/Beta
Region
BETA CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA1413
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.25 mg loader
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loader
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Hamster anti mouse TCR α/β, clone H57-597, recognizes the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne 1990).

Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Affinity purified murine T cell receptor.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 0.2-1ug/106 cells
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP
Goat F(ab')2 anti Hamster IgG:DyLight®550 STAR104D550 F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

References for TCR Alpha/Beta antibody

  1. Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
    J Immunol. 142 (8): 2736-42.
  2. Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
    J Exp Med. 170 (5): 1569-81.
  3. Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
    J Biol Chem. 265 (16): 9296-301.
  4. van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
    J Immunol. 154 (8): 3985-90.
  5. Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
    J Exp Med. 178 (3): 971-84.
  6. Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
    Glia. 59 (1): 45-57.
  7. Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
    PLoS One. 7 (7): e40657.

RRID
AB_322433
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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