TCR Alpha/Beta antibody | H57-597

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Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 thumbnail image 1

Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 thumbnail image 2

Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 gallery image 1 — Staining of mouse spleen cells with Hamster anti Mouse TCR alpha/beta (**MCA1413**).

Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 gallery image 2 — Staining of mouse spleen cells with Hamster anti Mouse TCR Alpha/Beta:FITC (**MCA1413F**).

Hamster anti Mouse TCR Alpha/Beta Beta Chain:FITC

Hamster anti Mouse TCR Alpha/Beta Beta Chain

Product Type: Monoclonal Antibody
Clone: H57-597
Isotype: IgG

Product Code	Applications	Pack Size	Your Price	Quantity
MCA1413F Datasheet	F SDS	0.1 mg	Log in	Get Quote
MCA1413FB Datasheet	F SDS	0.5 mg	Log in	Get Quote
MCA1413 Datasheet	F IP SDS	0.25 mg	Log in	Get Quote

Hamster anti mouse TCR α/β, clone H57-597, recognizes the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne 1990).

Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.

Product Details

Target Species

Mouse

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Affinity purified murine T cell receptor.

Approx. Protein Concentrations

Pack Size: 0.1 mg

IgG concentration 0.1 mg/ml

Pack Size: 0.5 mg

IgG concentration 0.5 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

Regulatory: For research purposes only

Applications of TCR Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/5	1/10 Pack Size: 0.1 mg Neat Pack Size: 0.5 mg
Flow Cytometry		0.2-1ug/10⁶ cells
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800	STAR104D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat F(ab')2 anti Hamster IgG:FITC	STAR104F	F	0.4 mg	Log in	Get Quote
Goat anti Hamster IgG:Biotin	STAR79B	E F	0.8 mg	Log in	Get Quote
Goat anti Hamster IgG:FITC	STAR79F	F	0.7 mg	Log in	Get Quote
Goat anti Hamster IgG:HRP	STAR79P	E	0.8 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Hamster (Armenian) IgG Negative Control:FITC	MCA2356F	F	0.1 mg	Log in		Get Quote
Hamster (Armenian) IgG Negative Control	MCA2356	F	0.25 mg	Log in		Get Quote

Product Specific References

References for TCR Alpha/Beta antibody

Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
J Immunol. 142 (8): 2736-42.
Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
J Exp Med. 170 (5): 1569-81.
Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
J Biol Chem. 265 (16): 9296-301.
van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
J Immunol. 154 (8): 3985-90.
Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
J Exp Med. 178 (3): 971-84.
Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
Glia. 59 (1): 45-57.
Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
PLoS One. 7 (7): e40657.

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