TCR Alpha/Beta antibody | H57-597


Hamster anti Mouse TCR Alpha/Beta Beta Chain:FITC
Hamster anti Mouse TCR Alpha/Beta Beta Chain
- Product Type
- Monoclonal Antibody
- Clone
- H57-597
- Isotype
- IgG
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1413F | F | 0.1 mg |
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MCA1413FB | F | 0.5 mg |
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MCA1413 | F IP | 0.25 mg |
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Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays. Slide-A-Lyzer® Dialysis Cassette is recommended for this purpose.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Affinity purified murine T cell receptor.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.
Storage Information
- Storage
- Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of TCR Alpha/Beta antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/5 | 1/10 Pack Size: 0.1 mg Neat Pack Size: 0.5 mg |
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Flow Cytometry | 0.2-1ug/106 cells | ||
Immunoprecipitation |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
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Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
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Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
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Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
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Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | F | 0.1 mg |
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Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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Product Specific References
References for TCR Alpha/Beta antibody
-
Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
J Immunol. 142 (8): 2736-42. -
Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
J Exp Med. 170 (5): 1569-81. -
Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
J Biol Chem. 265 (16): 9296-301. -
van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
J Immunol. 154 (8): 3985-90. -
Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
J Exp Med. 178 (3): 971-84. -
Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
Glia. 59 (1): 45-57. -
Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
PLoS One. 7 (7): e40657.
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