TCR Alpha/Beta antibody | H57-597

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Hamster anti Mouse TCR Alpha/Beta Beta Chain:FITC

Hamster anti Mouse TCR Alpha/Beta Beta Chain

Product Type
Monoclonal Antibody
Clone
H57-597
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1413F F 0.1 mg
MCA1413FB F 0.5 mg
MCA1413 F IP 0.25 mg
Hamster anti mouse TCR α/β, clone H57-597, recognises the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne, N., 1990).

Clone H57-597 has been reported to activate T-cells in immobilised form (Kubo, R. et al., 1989) and has also been used for in vivo cell depletion (van der Heyde, H. et al., 1995 and Skeen, M. & Ziegler, H., 1993). Removal of sodium azide is recommended prior to using this clone in functional assays. Bio-Rad recommend the Slide-A-Lyzer® Dialysis Cassette for this purpose.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Affinity purified murine T cell receptor.
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.

Storage Information

Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of TCR Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10 Pack Size: 0.1 mg
Neat Pack Size: 0.5 mg
Flow Cytometry 0.2-1ug/106 cells
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F 0.1 mg F
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Product Specific References

References for TCR Alpha/Beta antibody

  1. Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
    J Immunol. 142 (8): 2736-42.
  2. Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
    J Exp Med. 170 (5): 1569-81.
  3. Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
    J Biol Chem. 265 (16): 9296-301.
  4. van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
    J Immunol. 154 (8): 3985-90.
  5. Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
    J Exp Med. 178 (3): 971-84.
  6. Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
    Glia. 59 (1): 45-57.
  7. Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
    PLoS One. 7 (7): e40657.