TCR Alpha/Beta antibody | H57-597
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F ResetHamster anti Mouse TCR Alpha/Beta Beta Chain
- Product Type
- Monoclonal Antibody
- Clone
- H57-597
- Isotype
- IgG
- Specificity
- TCR Alpha/Beta
- Region
- BETA CHAIN
Antibody Quality and Performance Guarantee
| Hamster anti Mouse TCR α/β, clone H57-597, recognizes the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne 1990). Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al. 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Affinity purified murine T cell receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
0.2-1ug/106 cells | |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| List Price | Your Price | ||||||
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| Description | Hamster (Armenian) IgG Negative Control | ||||||
Source Reference
-
Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
J Immunol. 142 (8): 2736-42.
References for TCR Alpha/Beta antibody
-
Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
J Exp Med. 170 (5): 1569-81. -
Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
J Biol Chem. 265 (16): 9296-301. -
Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
J Exp Med. 178 (3): 971-84. -
van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
J Immunol. 154 (8): 3985-90. -
Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
Glia. 59 (1): 45-57.
- RRID
- AB_322433
MCA1413
154553
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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