TCR Alpha/Beta antibody | H57-597
Hamster anti mouse TCR α/β, clone H57-597, recognises the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne, N., 1990).
Clone H57-597 has been reported to activate T-cells in immobilised form (Kubo, R. et al., 1989) and has also been used for in vivo cell depletion (van der Heyde, H. et al., 1995 and Skeen, M. & Ziegler, H., 1993). Removal of sodium azide is recommended prior to using this clone in functional assays. Bio-Rad recommend the Slide-A-Lyzer® Dialysis Cassette for this purpose.
Clone H57-597 has been reported to activate T-cells in immobilised form (Kubo, R. et al., 1989) and has also been used for in vivo cell depletion (van der Heyde, H. et al., 1995 and Skeen, M. & Ziegler, H., 1993). Removal of sodium azide is recommended prior to using this clone in functional assays. Bio-Rad recommend the Slide-A-Lyzer® Dialysis Cassette for this purpose.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Affinity purified murine T cell receptor.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.
Storage Information
- Storage
- Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of TCR Alpha/Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 Pack Size: 0.1 mg Neat Pack Size: 0.5 mg |
|
| Flow Cytometry | 0.2-1ug/106 cells | ||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:DyLight®549 | STAR104D549GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:DyLight®649 | STAR104D649GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | 0.1 mg | F IF WB | ||
| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | 0.4 mg | F | ||
| Goat anti Hamster IgG:Biotin | STAR79B | 0.8 mg | E F | ||
| Goat anti Hamster IgG:FITC | STAR79F | 0.7 mg | F | ||
| Goat anti Hamster IgG:HRP | STAR79P | 0.8 mg | E |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | 0.1 mg | F | ||
| Hamster (Armenian) IgG Negative Control | MCA2356 | 0.25 mg | F |
Product Specific References
References for TCR Alpha/Beta antibody
-
Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
J Immunol. 142 (8): 2736-42. -
Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
J Exp Med. 170 (5): 1569-81. -
Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
J Biol Chem. 265 (16): 9296-301. -
van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
J Immunol. 154 (8): 3985-90. -
Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
J Exp Med. 178 (3): 971-84. -
Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
Glia. 59 (1): 45-57. -
Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
PLoS One. 7 (7): e40657.
