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TCR Alpha/Beta antibody | H57-597

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Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 thumbnail image 1 Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 thumbnail image 2
Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 gallery image 1
Staining of mouse spleen cells with Hamster anti Mouse TCR alpha/beta (MCA1413).
Anti Mouse TCR Alpha/Beta Beta Chain Antibody, clone H57-597 gallery image 2
Staining of mouse spleen cells with Hamster anti Mouse TCR Alpha/Beta:FITC (MCA1413F).

Hamster anti Mouse TCR Alpha/Beta Beta Chain:FITC

Hamster anti Mouse TCR Alpha/Beta Beta Chain

Product Type
Monoclonal Antibody
Clone
H57-597
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA1413F
Datasheet
F
SDS
0.1 mg loader

MCA1413FB
Datasheet
F
SDS
0.5 mg loader

MCA1413
Datasheet
F IP
SDS
0.25 mg loader

Hamster anti mouse TCR α/β, clone H57-597, recognizes the β-chain of the murine α/β T cell receptor and does not show any reactivity to TCRγ/δ (Gascoigne 1990).

Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Affinity purified murine T cell receptor.
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of TCR Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10 Pack Size: 0.1 mg
Neat Pack Size: 0.5 mg
Flow Cytometry 0.2-1ug/106 cells
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader

Product Specific References

References for TCR Alpha/Beta antibody

  1. Kubo, R.T. et al. (1989) Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
    J Immunol. 142 (8): 2736-42.
  2. Goodman, T. & Lefrancois, L. (1989) Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function.
    J Exp Med. 170 (5): 1569-81.
  3. Gascoigne, N.R. (1990) Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.
    J Biol Chem. 265 (16): 9296-301.
  4. van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
    J Immunol. 154 (8): 3985-90.
  5. Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
    J Exp Med. 178 (3): 971-84.
  6. Nilsson, I.A. et al. (2011) Evidence of hypothalamic degeneration in the anorectic anx/anx mouse.
    Glia. 59 (1): 45-57.
  7. Lenzen, H. et al. (2012) Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient mice.
    PLoS One. 7 (7): e40657.

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