IgG2a antibody | AbD24124
25 μg of HeLa cell lysate spiked with a primary antibody mixture of Mouse IgG1 anti actin beta (VMA00048), Mouse IgG2a anti PCNA (VMA00018) and Mouse IgG2b anti ezrin (VMA00344) each at 0.5 μg/ml were run in lanes 2, 4, 6, 8 and 10 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to PVDF membrane. Bio-Rad Precision Plus MW marker was run in lanes 1, 3, 5, 7, 9 and 11.
Mouse anti actin beta (VMA00048) 42 kDa, was detected using Human anti Mouse IgG1 conjugated to DyLight488 (clone AbD24121, HCA309D488) in lane 4. Clone AbD24121 does not recognize Mouse anti PCNA IgG2a or Mouse anti ezrin IgG2b.
Mouse anti PCNA (VMA00018) 29 kDa, was detected using Human anti Mouse IgG2a conjugated to DyLight650 (clone AbD24124, HCA310D650) in lane 6. Clone AbD24124 does not recognize Mouse anti actin beta IgG1 or Mouse anti ezrin IgG2b.
Mouse anti ezrin (VMA00344) 80 kDa, was detected using Human anti Mouse IgG2b conjugated to DyLight550 (clone AbD24127, HCA311D550) in lane 8. Clone AbD24127 does not recognize Mouse anti actin beta IgG1 or Mouse anti PCNA IgG2a.
Goat anti Mouse IgG antibody conjugated to Dylight549 (STAR117D549GA) was added to lane 10 as a standard antibody which detects all three isotypes.
All detection antibodies were used at 0.3 μg/ml. The blot was visualized using the Chemidoc imaging system.
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F IF P E WB EN ResetHuman anti Mouse IgG2a:DyLight®550
- Product Type
- Monoclonal Antibody
- Clone
- AbD24124
- Isotype
- HuCAL Fab bivalent
- Specificity
- IgG2a
Antibody Quality and Performance Guarantee
| Human anti Mouse IgG2a monoclonal antibody, clone AbD24124, recognizes the IgG2a isotype of the primary antibody, with no species cross reactivity against mouse IgG1 and mouse IgG2b primary antibodies. This antibody allows for more than one unlabelled mouse monoclonal antibody to be used at any one time permitting multiplexing. Human anti Mouse IgG2a antibody, clone AbD24124, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology. |
|
- Target Species
- Mouse
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to DyLight 550 - liquid.
- Preparation
- Prepared by affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Dylight®550 562 576 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.
DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
CelSelect Slides is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions.
The Lightning Link® technology used in the creation of this product is provided under an intellectual property license from Abcam Limited. For information on purchasing a license to this technology for purposes other than research contact Abcam at partnership@abcam.com. Lightning Link® is a registered trademark of Abcam Limited.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/500 | 1/10,000 |
| Immunofluorescence | |
1/100 | 1/1000 |
| Rare Cell / CTC Enumeration | |
1/100 | 1/500 |
| Western Blotting | |
1/1000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
HCA310D550
147935
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