IgG antibody | COCKTAIL
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Rat anti Mouse IgG Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- COCKTAIL
- Isotype
- Cocktail
- Specificity
- IgG
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgG Heavy Chain antibody cocktail has been shown to be specific for the gamma heavy chain of mouse immunoglobulin by immunoblotting techniques. It recognizes IgG2a and 2b strongly and IgG1 and IgG3 more weakly. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on mouse IgG/Sepharose 4B from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified IgG from BALB/c mice.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.25ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG antibody
-
Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
Prot. Biol. Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-618. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum
J. Immunol. Methods 71: 9 -16
- RRID
- AB_321924
- UniProt
- P01869
- P01865
- P03987
- P01867
- P01864
- P01868
- Entrez Gene
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0003823 antigen binding
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- View More GO Terms
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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