IgG antibody | COCKTAIL

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Rat anti Mouse IgG Heavy Chain:Biotin

Rat anti Mouse IgG Heavy Chain

Product Type
Monoclonal Antibody
Clone
COCKTAIL
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA424B E 0.5 mg
MCA424 E 0.25 mg
Rat anti Mouse IgG Heavy Chain antibody cocktail has been shown to be specific for the gamma heavy chain of mouse immunoglobulin by immunoblotting techniques. It recognizes IgG2a and 2b strongly and IgG1 and IgG3 more weakly.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on mouse Ig/Sepharose 4B from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on mouse IgG/Sepharose 4B from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified IgG from BALB/c mice.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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Entrez Gene
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GO Terms
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0ug/ml
ELISA 1.25ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgG antibody

  1. Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
    Prot. Biol. Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-618.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum
    J. Immunol. Methods 71: 9 -16