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High Mobility Group Protein B1 antibody | HMG1-5H6

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Anti High Mobility Group Protein B1 Antibody, clone HMG1-5H6 thumbnail image 1 Anti High Mobility Group Protein B1 Antibody, clone HMG1-5H6 thumbnail image 2 Anti High Mobility Group Protein B1 Antibody, clone HMG1-5H6 thumbnail image 3
Anti High Mobility Group Protein B1 Antibody, clone HMG1-5H6 gallery image 1
Western Blotting analysis of proteins and cell line lysates probed with Hamster anti Mouse high mobility group protein B1 (MCA5765)
Anti High Mobility Group Protein B1 Antibody, clone HMG1-5H6 gallery image 2
Immunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1 (MCA5765), signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:AlexaFluor594
Anti High Mobility Group Protein B1 Antibody, clone HMG1-5H6 gallery image 3
Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 (MCA5765) followed by Goat anti Hamster IgG:PE

Hamster anti High Mobility Group Protein B1

Product Type
Monoclonal Antibody
Clone
HMG1-5H6
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA5765GA
Datasheet
F IF WB
SDS
0.1 mg loader

Hamster anti High Mobility Group Protein B1 antibody, clone HMG1-5H6 specifically recognizes High mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein, and one of a newly emerging group of alarmins, which acts as a stabilizer of nucleosome formation, and facilitates transcription factor binding, by bending DNA.

HMGB1 is one of the signature danger signals of endogenous cellular injury, and is released outside the cell by necrotic and inflammatory cells to act as a chemoattractant for immature dendritic cells (DCs), promoting their maturation. DCs can also secrete HMGB1, promoting proliferation and Th1 polarization of interacting T cells. Cellular injury resulting in necrosis, leads to passive HMGB1 release, and microbes or pro-inflammatory cytokines may later stimulate active release from APCs.

HMGB1 is emerging as a prime specific marker and regulator of necrotic cell death, possibly through the PI3KC3-MEK-ERK pathway. It interacts directly with the autophagy protein Beclin-1, and binds to receptors, such as RAGE on endothelial cells, and Toll-like receptors on macrophages. Studies identifying microtubule-associated protein 1 light chain 3 (LC3) lipidation and redistribution, coupled with the accumulation of autophagosomes and autolysosomes, have shown an important role for HMGB1 release in sustaining autophagy. Studies have also shown that HMGB1 released after chemotherapy treatment is a critical regulator of autophagy, and a potential drug target for therapeutic interventions in leukemia.

HMGB1 is increasingly recognized as an important protein in medical research. It is angiogenic and promotes cardiac stem cell growth and differentiation, it may act as an adjuvant or assist in tissue repair, and is also a prototypical damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24 and is associated with the hallmarks of cancer.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
Hamster
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant mouse HMGB1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P63158
P63159
P09429
Entrez Gene
HMGB1
Hmgb1
Hmgb1
GO Terms
GO:0000793 condensed chromosome
GO:0001773 myeloid dendritic cell activation
GO:0002407 dendritic cell chemotaxis
GO:0002437 inflammatory response to antigenic stimulus
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006265 DNA topological change
GO:0006288 base-excision repair, DNA ligation
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0008301 DNA bending activity
GO:0009986 cell surface
GO:0017055 negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
GO:0031175 neuron projection development
GO:0033151 V(D)J recombination
GO:0042056 chemoattractant activity
GO:0043065 positive regulation of apoptosis
GO:0043280 positive regulation of caspase activity
GO:0043388 positive regulation of DNA binding
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050786 RAGE receptor binding
GO:0070491 repressing transcription factor binding
GO:0001654 eye development
GO:0003677 DNA binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005694 chromosome
GO:0008201 heparin binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010858 calcium-dependent protein kinase regulator activity
GO:0030295 protein kinase activator activity
GO:0030324 lung development
GO:0043005 neuron projection
GO:0045639 positive regulation of myeloid cell differentiation
GO:0045819 positive regulation of glycogen catabolic process
GO:0051384 response to glucocorticoid stimulus
GO:0000401 open form four-way junction DNA binding
GO:0000402 crossed form four-way junction DNA binding
GO:0000902 cell morphogenesis
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003681 bent DNA binding
GO:0050831 male-specific defense response to bacterium
GO:0006935 chemotaxis
GO:0007623 circadian rhythm
GO:0008097 5S rRNA binding
GO:0008134 transcription factor binding
GO:0008156 negative regulation of DNA replication
GO:0042493 response to drug
GO:0009408 response to heat
GO:0009749 response to glucose stimulus
GO:0010976 positive regulation of neuron projection development
GO:0014911 positive regulation of smooth muscle cell migration
GO:0031532 actin cytoskeleton reorganization
GO:0032392 DNA geometric change
GO:0032496 response to lipopolysaccharide
GO:0032868 response to insulin stimulus
GO:0033034 positive regulation of myeloid cell apoptosis
GO:0034341 response to interferon-gamma
GO:0042277 peptide binding
GO:0045663 positive regulation of myoblast differentiation
GO:0045931 positive regulation of mitotic cell cycle
GO:0046983 protein dimerization activity
GO:0050727 regulation of inflammatory response
GO:0050930 induction of positive chemotaxis
GO:0051450 myoblast proliferation
GO:0051861 glycolipid binding
GO:0071347 cellular response to interleukin-1
Regulatory
For research purposes only

Applications of High Mobility Group Protein B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
MCA5765GA detects a band of approximately 30kDa in mouse NIH-3T3, rat NRK, hamster BHK, and HeLa cell lysates in the presence of 2ME, and bands of 30kDa (red) and 27kDa (ox) in the absence of 2ME.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader

Product Specific References

Further Reading

  1. Lange, S.S. et al (2009) HMGB1: the jack-of-all-trades protein is a master DNA repair mechanic.
    Mol Carcinog. 48: 571-80.

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