High Mobility Group Protein B1 antibody | HMG1-5H6
Hamster anti High Mobility Group Protein B1 antibody, clone HMG1-5H6 specifically recognizes High mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein, and one of a newly emerging group of alarmins, which acts as a stabilizer of nucleosome formation, and facilitates transcription factor binding, by bending DNA.
HMGB1 is one of the signature danger signals of endogenous cellular injury, and is released outside the cell by necrotic and inflammatory cells to act as a chemoattractant for immature dendritic cells (DCs), promoting their maturation. DCs can also secrete HMGB1, promoting proliferation and Th1 polarization of interacting T cells. Cellular injury resulting in necrosis, leads to passive HMGB1 release, and microbes or pro-inflammatory cytokines may later stimulate active release from APCs.
HMGB1 is emerging as a prime specific marker and regulator of necrotic cell death, possibly through the PI3KC3-MEK-ERK pathway. It interacts directly with the autophagy protein Beclin-1, and binds to receptors, such as RAGE on endothelial cells, and Toll-like receptors on macrophages. Studies identifying microtubule-associated protein 1 light chain 3 (LC3) lipidation and redistribution, coupled with the accumulation of autophagosomes and autolysosomes, have shown an important role for HMGB1 release in sustaining autophagy. Studies have also shown that HMGB1 released after chemotherapy treatment is a critical regulator of autophagy, and a potential drug target for therapeutic interventions in leukemia.
HMGB1 is increasingly recognized as an important protein in medical research. It is angiogenic and promotes cardiac stem cell growth and differentiation, it may act as an adjuvant or assist in tissue repair, and is also a prototypical damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24 and is associated with the hallmarks of cancer.
HMGB1 is one of the signature danger signals of endogenous cellular injury, and is released outside the cell by necrotic and inflammatory cells to act as a chemoattractant for immature dendritic cells (DCs), promoting their maturation. DCs can also secrete HMGB1, promoting proliferation and Th1 polarization of interacting T cells. Cellular injury resulting in necrosis, leads to passive HMGB1 release, and microbes or pro-inflammatory cytokines may later stimulate active release from APCs.
HMGB1 is emerging as a prime specific marker and regulator of necrotic cell death, possibly through the PI3KC3-MEK-ERK pathway. It interacts directly with the autophagy protein Beclin-1, and binds to receptors, such as RAGE on endothelial cells, and Toll-like receptors on macrophages. Studies identifying microtubule-associated protein 1 light chain 3 (LC3) lipidation and redistribution, coupled with the accumulation of autophagosomes and autolysosomes, have shown an important role for HMGB1 release in sustaining autophagy. Studies have also shown that HMGB1 released after chemotherapy treatment is a critical regulator of autophagy, and a potential drug target for therapeutic interventions in leukemia.
HMGB1 is increasingly recognized as an important protein in medical research. It is angiogenic and promotes cardiac stem cell growth and differentiation, it may act as an adjuvant or assist in tissue repair, and is also a prototypical damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24 and is associated with the hallmarks of cancer.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human Hamster - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant mouse HMGB1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P63158
- P63159
- P09429
- Entrez Gene
- HMGB1
- Hmgb1
- Hmgb1
- GO Terms
- GO:0000793 condensed chromosome
- GO:0001773 myeloid dendritic cell activation
- GO:0002407 dendritic cell chemotaxis
- GO:0002437 inflammatory response to antigenic stimulus
- GO:0003684 damaged DNA binding
- GO:0003690 double-stranded DNA binding
- GO:0003697 single-stranded DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006265 DNA topological change
- GO:0006288 base-excision repair, DNA ligation
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0008301 DNA bending activity
- GO:0009986 cell surface
- GO:0017055 negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
- GO:0031175 neuron projection development
- GO:0033151 V(D)J recombination
- GO:0042056 chemoattractant activity
- GO:0043065 positive regulation of apoptosis
- GO:0043280 positive regulation of caspase activity
- GO:0043388 positive regulation of DNA binding
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0050786 RAGE receptor binding
- GO:0070491 repressing transcription factor binding
- GO:0001654 eye development
- GO:0003677 DNA binding
- GO:0005625 soluble fraction
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005694 chromosome
- GO:0008201 heparin binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010858 calcium-dependent protein kinase regulator activity
- GO:0030295 protein kinase activator activity
- GO:0030324 lung development
- GO:0043005 neuron projection
- GO:0045639 positive regulation of myeloid cell differentiation
- GO:0045819 positive regulation of glycogen catabolic process
- GO:0051384 response to glucocorticoid stimulus
- GO:0000401 open form four-way junction DNA binding
- GO:0000402 crossed form four-way junction DNA binding
- GO:0000902 cell morphogenesis
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003681 bent DNA binding
- GO:0050831 male-specific defense response to bacterium
- GO:0006935 chemotaxis
- GO:0007623 circadian rhythm
- GO:0008097 5S rRNA binding
- GO:0008134 transcription factor binding
- GO:0008156 negative regulation of DNA replication
- GO:0042493 response to drug
- GO:0009408 response to heat
- GO:0009749 response to glucose stimulus
- GO:0010976 positive regulation of neuron projection development
- GO:0014911 positive regulation of smooth muscle cell migration
- GO:0031532 actin cytoskeleton reorganization
- GO:0032392 DNA geometric change
- GO:0032496 response to lipopolysaccharide
- GO:0032868 response to insulin stimulus
- GO:0033034 positive regulation of myeloid cell apoptosis
- GO:0034341 response to interferon-gamma
- GO:0042277 peptide binding
- GO:0045663 positive regulation of myoblast differentiation
- GO:0045931 positive regulation of mitotic cell cycle
- GO:0046983 protein dimerization activity
- GO:0050727 regulation of inflammatory response
- GO:0050930 induction of positive chemotaxis
- GO:0051450 myoblast proliferation
- GO:0051861 glycolipid binding
- GO:0071347 cellular response to interleukin-1
- Regulatory
- For research purposes only
Applications of High Mobility Group Protein B1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunofluorescence | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA5765GA detects a band of approximately 30kDa in mouse NIH-3T3, rat NRK, hamster BHK, and HeLa cell lysates in the presence of 2ME, and bands of 30kDa (red) and 27kDa (ox) in the absence of 2ME.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
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| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
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| Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
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|
| Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
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|
| Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
|
Product Specific References
Further Reading
-
Lange, S.S. et al (2009) HMGB1: the jack-of-all-trades protein is a master DNA repair mechanic.
Mol Carcinog. 48: 571-80.
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