High Mobility Group Protein B1 antibody | HMG1-5H6

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunofluorescence
Western Blotting

Flow Cytometry

Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Western Blotting

MCA5765GA detects a band of approximately 30kDa in mouse NIH-3T3, rat NRK, hamster BHK, and HeLa cell lysates in the presence of 2ME, and bands of 30kDa (red) and 27kDa (ox) in the absence of 2ME.

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800	STAR104D800GA	F IF WB	0.1 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC	STAR104F	F	0.4 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin	STAR79B	E F	0.8 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC	STAR79F	F	0.7 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP	STAR79P	E	0.8 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Goat anti Hamster IgG:HRP

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Hamster (Armenian) IgG Negative Control	MCA2356	F	0.25 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Hamster (Armenian) IgG Negative Control

Further Reading

1. Lange, S.S. et al (2009) HMGB1: the jack-of-all-trades protein is a master DNA repair mechanic.
   Mol Carcinog. 48: 571-80.

Synonyms

HMGB1

RRID

AB_10844211

UniProt

P63158

P63159

P09429

Entrez Gene

HMGB1

Hmgb1

Hmgb1

GO Terms

GO:0000793 condensed chromosome

GO:0001773 myeloid dendritic cell activation

GO:0002407 dendritic cell chemotaxis

GO:0002437 inflammatory response to antigenic stimulus

GO:0003684 damaged DNA binding

GO:0003690 double-stranded DNA binding

GO:0003697 single-stranded DNA binding

GO:0003700 sequence-specific DNA binding transcription factor activity

GO:0005125 cytokine activity

View More GO Terms

GO:0005615 extracellular space

GO:0005654 nucleoplasm

GO:0005730 nucleolus

GO:0006265 DNA topological change

GO:0006288 base-excision repair, DNA ligation

GO:0006309 DNA fragmentation involved in apoptotic nuclear change

GO:0008301 DNA bending activity

GO:0009986 cell surface

GO:0017055 negative regulation of RNA polymerase II transcriptional preinitiation complex assembly

GO:0031175 neuron projection development

GO:0033151 V(D)J recombination

GO:0042056 chemoattractant activity

GO:0043065 positive regulation of apoptosis

GO:0043280 positive regulation of caspase activity

GO:0043388 positive regulation of DNA binding

GO:0045087 innate immune response

GO:0045944 positive regulation of transcription from RNA polymerase II promoter

GO:0050786 RAGE receptor binding

GO:0070491 repressing transcription factor binding

GO:0001654 eye development

GO:0003677 DNA binding

GO:0005625 soluble fraction

GO:0005634 nucleus

GO:0005737 cytoplasm

GO:0005694 chromosome

GO:0008201 heparin binding

GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter

GO:0010858 calcium-dependent protein kinase regulator activity

GO:0030295 protein kinase activator activity

GO:0030324 lung development

GO:0043005 neuron projection

GO:0045639 positive regulation of myeloid cell differentiation

GO:0045819 positive regulation of glycogen catabolic process

GO:0051384 response to glucocorticoid stimulus

GO:0000401 open form four-way junction DNA binding

GO:0000402 crossed form four-way junction DNA binding

GO:0000902 cell morphogenesis

GO:0002053 positive regulation of mesenchymal cell proliferation

GO:0003681 bent DNA binding

GO:0050831 male-specific defense response to bacterium

GO:0006935 chemotaxis

GO:0007623 circadian rhythm

GO:0008097 5S rRNA binding

GO:0008134 transcription factor binding

GO:0008156 negative regulation of DNA replication

GO:0042493 response to drug

GO:0009408 response to heat

GO:0009749 response to glucose stimulus

GO:0010976 positive regulation of neuron projection development

GO:0014911 positive regulation of smooth muscle cell migration

GO:0031532 actin cytoskeleton reorganization

GO:0032392 DNA geometric change

GO:0032496 response to lipopolysaccharide

GO:0032868 response to insulin stimulus

GO:0033034 positive regulation of myeloid cell apoptosis

GO:0034341 response to interferon-gamma

GO:0042277 peptide binding

GO:0045663 positive regulation of myoblast differentiation

GO:0045931 positive regulation of mitotic cell cycle

GO:0046983 protein dimerization activity

GO:0050727 regulation of inflammatory response

GO:0050930 induction of positive chemotaxis

GO:0051450 myoblast proliferation

GO:0051861 glycolipid binding

GO:0071347 cellular response to interleukin-1

MCA5765GA

160721 171005

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