Delta-Like Protein 4 antibody | HMD4-2

Hamster anti Mouse Delta-Like Protein 4

Product Type
Monoclonal Antibody
Clone
HMD4-2
Isotype
IgG
Specificity
Delta-Like Protein 4

Product Code Applications Pack Size List Price Your Price Qty
MCA5706
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN P * 0.25 mg loader
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loader
MCA5706GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN P * 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Hamster anti Mouse Delta-Like Protein 4 antibody, clone HMD4-2 recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signaling pathway, activated through the binding of specific ligands to the Notch receptors Notch 1-4.

DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signaling has been shown to play a role in the angiogenesis of clear-cell renal tumors, and pancreatic, bladder and colonic cancer. DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non-productive angiogenesis.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
MCA5706: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA5706GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant mouse DLL4.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Mouse spleen

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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP
Goat F(ab')2 anti Hamster IgG:DyLight®550 STAR104D550 F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

References for Delta-Like Protein 4 antibody

  1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
    Int Immunol. 20 (6): 763-73.
  2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
    Int Immunol. 21 (3): 295-301.
  3. Yamanda, S. et al. (2009) Role of ephrinB2 in nonproductive angiogenesis induced by Delta-like 4 blockade.
    Blood. 113 (15): 3631-9.
  4. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
    Arthritis Res Ther. 14: R45.

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.
  2. Iso, T. et al. (2003) Notch signaling in vascular development.
    Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
  3. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
    Immunity. 29 (5): 691-703.
  4. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
    Immunol Rev. 182: 215-27.

Flow Cytometry

Functional Assays

Synonyms
DLL4
RRID
AB_10708982
UniProt
Q9JI71
Entrez Gene
Dll4
GO Terms
GO:0005509 calcium ion binding
GO:0001525 angiogenesis
GO:0016021 integral to membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0030154 cell differentiation
GO:0043537 negative regulation of blood vessel endothelial cell migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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