CD81 antibody | Eat2
Hamster anti Mouse CD81
- Product Type
- Monoclonal Antibody
- Clone
- Eat2
- Isotype
- IgG1
- Specificity
- CD81
Hamster anti Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding. Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006). |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- 38C13, murine B cell line.
- Approx. Protein Concentrations
- MCA1846, MCA1846T: IgG concentration 1.0 mg/ml
- MCA1846GA: IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Flow Cytometry | 1/50 Pack Size: 0.25 mg, 25 µg 1/10 Pack Size: 0.1 mg |
1/100 Pack Size: 0.25 mg, 25 µg 1/50 Pack Size: 0.1 mg |
|
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting 2 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2Clone Eat2 recognizes mouse CD81 under non-reducing conditions.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD81 antibody
-
Maecker, H.T. et al. (2000) Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies.
Hybridoma 19: 15-22. -
Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.
Cancer Res. 61: 5248-54. -
Clark, K.L. et al. (2001) PGRL is a major CD81-associated protein on lymphocytes and distinguishes a new family of cell surface proteins.
J Immunol. 167 (9): 5115-21. -
Ha, C.T. et al. (2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.
J Leukoc Biol. 77: 948-57. -
Takeda, Y. et al. (2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.
J Biol Chem. 283: 26089-97. -
Conde-Vancells, J. et al. (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.
J Proteome Res. 7: 5157-66. -
Suzuki, M. et al. (2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.
J Immunol. 182: 6485-93. -
Conde-Vancells, J. et al. (2010) Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Proteomics Clin Appl. 4 (4): 416-25.
View The Latest Product References
-
Pan, Q. et al. (2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).
Gut. 61: 1330-9. -
Sosa, L.J. et al. (2013) Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.
PLoS One. 8 (5): e64521. -
Royo, F. et al. (2013) Transcriptome of extracellular vesicles released by hepatocytes.
PLoS One. 8: e68693. -
Jin, Y. et al. (2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.
PLoS One. 8: e73706. -
Jin, Y. et al. (2018) Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.
Sci Rep. 8 (1): 5145.
- Synonyms
- TAPA-1
- RRID
- AB_323200
- UniProt
- P35762
- Entrez Gene
- Cd81
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0008104 protein localization
- GO:0005887 integral to plasma membrane
- GO:0006661 phosphatidylinositol biosynthetic process
- GO:0030307 positive regulation of cell growth
- GO:0030890 positive regulation of B cell proliferation
- GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- View More GO Terms
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