CD79b antibody | HM79-11
FITC conjugated Hamster anti Mouse CD79β antibody (MCA1821F) used for the evaluation of CD79β expression on Ly6C positive cells by flow cytometry.
Image caption:
Expression of the B-cell receptor subunit CD79a on MDSCs induced by metastatic tumors.
CD79a/b expression on the Ly6C+ myeloid cell population in multiple tumor models was assessed by flow cytometry. (A) Expression of the B-cell markers CD19 and CD79a/b on splenocytes from naïve and 67NR and 4T1 tumor-bearing mice. (B) Ly6C and CD79a/b expression on viable leukocytes from lungs and spleens of Balb/c mice bearing 67NR or 4T1 tumors at day 28 post-inoculation. The box indicates the immature myeloid population. CD79 on myeloid cells is recognized by the CD79-11 but not the CD79-12 antibody. (C) CD79a expression on MDSCs was also evaluated in the Lewis Lung Carcinoma transplantable metastatic lung cancer model (C57Bl/6 background). (D,E) Similar evaluation was done in two genetically-engineered models of breast cancer; the metastatic MMTV-PyMT model (D) on the FVB/N background (age 3 months) and the non-metastatic Brca1 model (E) on a mixed genetic background (age 6 months).
From: Luger D, Yang Y-a, Raviv A, Weinberg D, Banerjee S, Lee M-J, et al. (2013)
Expression of the B-Cell Receptor Component CD79a on Immature Myeloid Cells Contributes to Their Tumor Promoting Effects.
PLoS ONE 8(10): e76115.
FITC conjugated Hamster anti Mouse CD79β antibody, clone HM79-11(MCA1821F) used for the evaluation of CD79β expression on bone marrow cells from naïe SCID and tumor bearing mice by flow cytometry.
Image caption:
Expression of CD79a mRNA and protein in bone marrow cells from B-cell deficient mice.
(A) RNA was extracted from BM cells of naïve SCID mice and from spleen of naïve Balb/c mice and CD79a and CD79b mRNA expression were measured by qRT-PCR. RNA levels were normalized to PPIAand presented relative to the level of CD79b mRNA in SCID BM. Results are mean +/− SEM; n = 3. (B) BM-derived leukocytes from naïve SCID mice were analyzed by flow cytometry for intracellular epitopes of CD79a using the anti-mouse CD79a clone F11-172. (C) To assess the extracellular expression of CD79a, BM cells from mice bearing LLC tumors were co-stained with anti CD79-11 together with the polyclonal anti-CD79a(v-20), which showed a weaker pattern of staining than CD79-11. Boxes indicate the myeloid cells. Data shown are representative of three independent experiments.
From: Luger D, Yang Y-a, Raviv A, Weinberg D, Banerjee S, Lee M-J, et al. (2013)
Expression of the B-Cell Receptor Component CD79a on Immature Myeloid Cells Contributes to Their Tumor Promoting Effects.
PLoS ONE 8(10): e76115.
Hamster anti Mouse CD79β antibody, clone HM79-11(MCA1821GA) used for the evaluation of CD79β expression splenocytes from naï and tumor bearing mice by western blotting.
Image caption:
Expression of CD79a mRNA and protein in bone marrow cells from B-cell deficient mice.
(A) RNA was extracted from BM cells of naïve SCID mice and from spleen of naïve Balb/c mice and CD79a and CD79b mRNA expression were measured by qRT-PCR. RNA levels were normalized to PPIAand presented relative to the level of CD79b mRNA in SCID BM. Results are mean +/− SEM; n = 3. (B) BM-derived leukocytes from naïve SCID mice were analyzed by flow cytometry for intracellular epitopes of CD79a using the anti-mouse CD79a clone F11-172. (C) To assess the extracellular expression of CD79a, BM cells from mice bearing LLC tumors were co-stained with anti CD79-11 together with the polyclonal anti-CD79a(v-20), which showed a weaker pattern of staining than CD79-11. Boxes indicate the myeloid cells.
From: Luger D, Yang Y-a, Raviv A, Weinberg D, Banerjee S, Lee M-J, et al. (2013)
Expression of the B-Cell Receptor Component CD79a on Immature Myeloid Cells Contributes to Their Tumor Promoting Effects.
PLoS ONE 8(10): e76115.
Hamster anti Mouse CD79b:Alexa Fluor® 488
Hamster anti Mouse CD79b:Alexa Fluor® 647
Hamster anti Mouse CD79b:FITC
Hamster anti Mouse CD79b
Hamster anti Mouse CD79b:RPE
- Product Type
- Monoclonal Antibody
- Clone
- HM79-11
- Isotype
- IgG
Hamster anti Mouse CD79b antibody, clone HM79-11 recognizes murine CD79 beta, expressed by B cells as part of the B cell receptor complex (immunoglobulin and the CD79 alpha/beta heterodimer).
Murine CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin, and is expressed throughout B cell differentiation.
CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages.
Murine CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin, and is expressed throughout B cell differentiation.
CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- CD79 alpha/CD79 beta heterodimer purified from WEHI-231 B cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Lymph node cells from immunised Armenian hamsters were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P15530 Related reagents
- Entrez Gene
- Cd79b Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0050853 B cell receptor signaling pathway
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD79b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:DyLight®549 | STAR104D549GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:DyLight®649 | STAR104D649GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | 0.1 mg | F IF WB | ||
| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | 0.4 mg | F | ||
| Goat anti Hamster IgG:Biotin | STAR79B | 0.8 mg | E F | ||
| Goat anti Hamster IgG:FITC | STAR79F | 0.7 mg | F | ||
| Goat anti Hamster IgG:HRP | STAR79P | 0.8 mg | E |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 | MCA2356A488 | 100 Tests/1ml | F | ||
| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 | MCA2356A647 | 100 Tests/1ml | F | ||
| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | 0.1 mg | F | ||
| Hamster (Armenian) IgG Negative Control | MCA2356 | 0.25 mg | F | ||
| Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | 0.1 mg | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Product Specific References
References for CD79b antibody
-
Koyama, M. et al. (1997) CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain.
Int Immunol. 9 (11): 1767-72. -
Luger, D. et al. (2013) Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects.
PLoS One. 8 (10): e76115. -
Jelínek, F. et al. (2005) Characteristics of two mouse bcr-abl-transformed cell lines. II. Pathological lesions induced in mice.
Folia Biol (Praha). 51 (4): 93-102. -
Ankeny, D.P. et al. (2006) Spinal cord injury triggers systemic autoimmunity: evidence for chronic B lymphocyte activation and lupus-like autoantibody synthesis.
J Neurochem. 99 (4): 1073-87. -
Lacoste-collin, L. et al. (2007) Effect of continuous irradiation with a very low dose of gamma rays on life span and the immune system in SJL mice prone to B-cell lymphoma.
Radiat Res. 168 (6): 725-32.
