CD4 antibody | RM4-5
Rat anti Mouse CD4 antibody, clone RM4-5 (MCA2691) used to identify T helper lymphocytes in murine brain frontal cortex by immunohistochemistry on floating sections.
Image caption:
TDP-43 overexpression induces blood-brain barrier permeability. a Representative images depicting CD3+ T cells in the frontal cortex of mice. Scale bar = 10 μm. b Manual count of CD3+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p <0.001). c Representative images depicting CD4+ T cells in the frontal cortex of mice. Scale bar = 10 &mum;m. d Manual count of CD4+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group; ***p <0.001). e Representative images depicting mouse IgG in the frontal cortex of mouse tissue. Scale bar = 20 μm. f Quantification of percent area of the frontal cortex covered by mouse IgG staining. Statistical analysis was carried out using a one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p <0.001).
From: Zamudio F, Loon AR, Smeltzer S, Benyamine K, Navalpur Shanmugam NK, Stewart NJF, Lee DC, Nash K, Selenica MB.
TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 2020 Sep 26;17(1):283.
doi: 10.1186/s12974-020-01952-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD4 antibody, clone RM4-5 (MCA2691) used to label T helper cells in murine neural tissues by immunohistochemistry on formalin fixed cryosections
Image caption:
Histology of EAE-affected mice upon AA treatment. Immunochemistry (IHC) semi-quantification was obtained by ImageJ Software (National Institutes of Health, Bethesda, MD, USA, https://imagej.nih.gov/ij/)
(E,F) Immune infiltrates ((E); CD4+ cells/mm2) and neuroinflammation ((F); CD68+ cells/mm2). The impact of AA treatment was statistically significant (p-values = 0.0303 and 0.0043 for t test and Mann−Whitney U test, respectively). The representative images related to each group of treatment were reported in 5× and 20× magnifications (IHC).
From: Camponeschi C, De Carluccio M, Amadio S, Clementi ME, Sampaolese B, Volonté C, Tredicine M, Romano Spica V, Di Liddo R, Ria F, Michetti F, Di Sante G. S100B Protein as a Therapeutic Target in Multiple Sclerosis:
The S100B Inhibitor Arundic Acid Protects from Chronic Experimental Autoimmune Encephalomyelitis.
Int J Mol Sci. 2021 Dec 17;22(24):13558.
doi: 10.3390/ijms222413558.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD4:StarBright Violet 610
- Product Type
- Monoclonal Antibody
- Clone
- RM4-5
- Isotype
- IgG2a
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD4 antibody, clone RM4-5 detects mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to StarBright Violet 610 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- BALB/c mouse thymocytes
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 610 403 607 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
References for CD4 antibody
-
von Kutzleben, S. et al. (2017) Depletion of CD52-positive cells inhibits the development of central nervous system autoimmune disease, but deletes an immune-tolerance promoting CD8 T-cell population. Implications for secondary autoimmunity of alemtuzumab in multiple sclerosis.
Immunology. 150 (4): 444-55. -
Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 17 (1): 283. -
Aloui, A. et al. (2023) AFM(1) Exposure in Male Balb/c Mice and Intervention Strategies Against Its Immuno-physiological toxicity using Clay Mineral and Lactic Acid Bacteria Alone or in Combination.
Immunopharmacol Immunotoxicol. : 1-32. -
Camponeschi, C. et al. (2021) S100B Protein as a Therapeutic Target in Multiple Sclerosis: The S100B Inhibitor Arundic Acid Protects from Chronic Experimental Autoimmune Encephalomyelitis.
Int J Mol Sci. 22 (24): 13558.
Further Reading
-
Fehérvari, Z. & Sakaguchi, S. (2004) CD4+ Tregs and immune control.
J Clin Invest. 114 (9): 1209-17.
- Synonyms
- L3T4 Antigen
- LY-4
- UniProt
- P06332
- Entrez Gene
- Cd4
- GO Terms
- GO:0001816 cytokine production
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0045058 T cell selection
- View More GO Terms
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050870 positive regulation of T cell activation
- GO:0051789 response to protein stimulus
MCA2691SBV610
100004879
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