CD4 antibody | RM4-5
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F C ResetRat anti Mouse CD4:StarBright Violet 610
- Product Type
- Monoclonal Antibody
- Clone
- RM4-5
- Isotype
- IgG2a
- Specificity
- CD4
Rat anti Mouse CD4 antibody, clone RM4-5 detects mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to StarBright Violet 610 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- BALB/c mouse thymocytes
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 610 403 607 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR |
References for CD4 antibody
-
von Kutzleben, S. et al. (2017) Depletion of CD52-positive cells inhibits the development of central nervous system autoimmune disease, but deletes an immune-tolerance promoting CD8 T-cell population. Implications for secondary autoimmunity of alemtuzumab in multiple sclerosis.
Immunology. 150 (4): 444-55. -
Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 17 (1): 283. -
Aloui, A. et al. (2023) AFM(1) Exposure in Male Balb/c Mice and Intervention Strategies Against Its Immuno-physiological toxicity using Clay Mineral and Lactic Acid Bacteria Alone or in Combination.
Immunopharmacol Immunotoxicol. : 1-32. -
Camponeschi, C. et al. (2021) S100B Protein as a Therapeutic Target in Multiple Sclerosis: The S100B Inhibitor Arundic Acid Protects from Chronic Experimental Autoimmune Encephalomyelitis.
Int J Mol Sci. 22 (24): 13558.
Further Reading
-
Fehérvari, Z. & Sakaguchi, S. (2004) CD4+ Tregs and immune control.
J Clin Invest. 114 (9): 1209-17.
- Synonyms
- L3T4 Antigen
- LY-4
- UniProt
- P06332
- Entrez Gene
- Cd4
- GO Terms
- GO:0001816 cytokine production
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0045058 T cell selection
- View More GO Terms
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050870 positive regulation of T cell activation
- GO:0051789 response to protein stimulus
MCA2691SBV610
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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