CD3 antibody | KT3

Rat anti Mouse CD3:StarBright Violet 570

Product Type
Monoclonal Antibody
Clone
KT3
Isotype
IgG2a
Specificity
CD3

Product Code Applications Pack Size List Price Your Price Qty
MCA500SBV570
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F 100 Tests/0.5ml loader
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Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).

NB. For optimal staining incubations should be performed at room temperature.

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
CBAT6 thymocytes
Fusion Partners
Spleen cells from immunized SD rats were fused with cells of the NS0 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 570 402 570
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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Mouse Seroblock FcR BUF041B F 0.5 mg loader
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References for CD3 antibody

  1. Tomonari, K. (1988) A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex.
    Immunogenetics. 28 (6): 455-8.
  2. Lacroix-Lamandé, S. et al. (2002) Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection.
    Infect Immun. 70 (4): 2090-9.
  3. Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
    Immunogenetics. 28 (6): 445-51.
  4. Haroon, F. et al., (2011) Gp130-dependent astrocytic survival is critical for the control of autoimmune central nervous system inflammation.
    J Immunol. 186: 6521-31.
  5. Kumar, L. et al. (2002) Differential role of SLP-76 domains in T cell development and function.
    Proc Natl Acad Sci U S A. 99: 884-9.
  6. Hirsh, M. et al. (2004) Response of lung gammadelta T cells to experimental sepsis in mice.
    Immunology. 112: 153-60.
  7. Tarlton, J.F. et al. (2000) The role of up-regulated serine proteases and matrix metalloproteinases in the pathogenesis of a murine model of colitis.
    Am J Pathol. 157: 1927-35.
  8. Lazarovits, A.I. et al. (1999) Mechanisms of induction of renal allograft tolerance in CD45RB-treated mice.
    Kidney Int. 55: 1303-10.
  9. View The Latest Product References
  10. Bauer, D. et al. (2009) Amniotic membrane transplantation induces apoptosis in T lymphocytes in murine corneas with experimental herpetic stromal keratitis
    Invest Ophthalmol Vis Sci. 50: 3188-98.
  11. Heitmann, S. et al. (1999) Immunohistological characterization of leukocytes in the lungs of healthy mice and after bacterial intratracheal infection.
    Lab Anim. 33: 288-94.
  12. Hare, K.J. et al. (2003) Modeling TCR signaling complex formation in positive selection.
    J Immunol. 171: 2825-31.
  13. Erlandsson, L. et al. (2004) Impaired B-1 and B-2 B cell development and atypical splenic B cell structures in IL-7 receptor-deficient mice.
    Eur J Immunol. 34: 3595-603.
  14. Severinová, J. et al. (2005) Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.
    Folia Microbiol (Praha). 50: 457-63.
  15. Rothhammer, V. et al. (2011) Th17 lymphocytes traffic to the central nervous system independently of α4 integrin expression during EAE.
    J Exp Med. 208 (12): 2465-76.
  16. Salem, M. et al. (2011) Interferon regulatory factor-7 modulates experimental autoimmune encephalomyelitis in mice.
    J Neuroinflammation. 8: 181.
  17. Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
    EMBO Mol Med. pii: e201404170.
  18. Scheinert, R.B. et al. (2016) Therapeutic effects of stress-programmed lymphocytes transferred to chronically stressed mice.
    Prog Neuropsychopharmacol Biol Psychiatry. Apr 21. pii: S0278-5846(16)30056-2. [Epub ahead of print]
  19. Schaut, R.G. et al. (2015) Bovine viral diarrhea virus type 2 in vivo infection modulates TLR4 responsiveness in differentiated myeloid cells which is associated with decreased MyD88 expression.
    Virus Res. 208: 44-55.
  20. Janssen, E. et al. (2016) A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton.
    J Clin Invest. 126 (10): 3837-51.
  21. Teeling, J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
    Acta Neuropathol. 124 (4): 479-90.
  22. Van Aelst, L.N. et al. (2016) RNA Profiling in Human and Murine Transplanted Hearts: Identification and Validation of Therapeutic Targets for Acute Cardiac and Renal Allograft Rejection.
    Am J Transplant. 16 (1): 99-110.
  23. Kim, I. et al. (2016) Immunological characterization of de novo and recall alloantibody suppression by CTLA4Ig in a mouse model of allosensitization.
    Transpl Immunol. 38: 84-92.
  24. Massa, M.G. et al. (2017) Testosterone Differentially Affects T Cells and Neurons in Murine and Human Models of Neuroinflammation and Neurodegeneration.
    Am J Pathol. 187 (7): 1613-22.
  25. Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
    J Neuroinflammation. 17 (1): 283.
  26. Yun, M. et al. (2020) Enriched-Baicalein Attenuates Allergy in Cells and Mice
    Ev-Based Comp Alt Med.. 2020: 1-8.
  27. Granadillo, M. et al. (2019) Impact on antitumor response using a new adjuvant preparation as a component of a human papillomavirus type 16 therapeutic vaccine candidate.
    Vaccine. 37 (30): 3957-60.

Flow Cytometry

Functional Assays

Immunofluorescence

Immunohistology - Frozen

UniProt
P22646
P24161
P11942
P29020
P04235
Entrez Gene
Cd3d
Cd3g
Cd3e
Cd247
Cd247
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0006461 protein complex assembly
GO:0007166 cell surface receptor linked signaling pathway
GO:0042105 alpha-beta T cell receptor complex
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway
GO:0005515 protein binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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