CD3 antibody | KT3
Rat anti Mouse CD3, clone KT3 (MCA500GA) used for the identification of CD3+ T cells in the brain of mice following ovalbumin innoculation by immunohistochemistry on acetone fixed tissue sections.
Image caption:
T-cell recruitment after immune complex formation. OVA-immunized mice received a unilateral injection of OVA into the striatum and brain tissue was assessed for presence of CD3-positive T cells at 24 h (a), day 7 (b) and day 14 (c) after OVA injection. Non-immunized mice were used as control (d, g). OVA-immunized wild type (e, h) and Fcγ−/− (f, i) mice received a unilateral injection of OVA into the striatum and tissue was analysed for CD3 (d, e, f) or CD11b (g, h, i) immunoreactivity at 7 days. Representative data of n = 2–3 per time point are shown. Scale bar 100μm
From: Teeling JL, Carare RO, Glennie MJ, Perry VH.
Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
Acta Neuropathol. 2012 Oct;124(4):479-90.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone KT3 (MCA500G) used to identify T lymphocytes in murine brain frontal cortex by immunohistochemistry on floating sections.
Image caption:
TDP-43 overexpression induces blood-brain barrier permeability. a Representative images depicting CD3+ T cells in the frontal cortex of mice. Scale bar = 10 μm. b Manual count of CD3+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p <0.001). c Representative images depicting CD4+ T cells in the frontal cortex of mice. Scale bar = 10 &mum;m. d Manual count of CD4+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group; ***p <0.001). e Representative images depicting mouse IgG in the frontal cortex of mouse tissue. Scale bar = 20 μm. f Quantification of percent area of the frontal cortex covered by mouse IgG staining. Statistical analysis was carried out using a one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p <0.001).
From: Zamudio F, Loon AR, Smeltzer S, Benyamine K, Navalpur Shanmugam NK, Stewart NJF, Lee DC, Nash K, Selenica MB.
TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 2020 Sep 26;17(1):283.
doi: 10.1186/s12974-020-01952-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone KT3 (MCA500G) used to coat plates and stimulate cells to measure cytokine production in vitro
Image caption:
Effect of baicalein on IL-4 and IL-13 production in the culture supernatant of CD3 stimulated splenocytes. Balb/c males (6 weeks old) were used and ovalbumin was injected intraperitoneally for 0, 7, 14, 21, and 28 days to Balb/c (n = 5). The baicalein, containing 100 mg of baicalein/kg body weight per day dissolved in 0.4% polyethylene glycol in 0.5% EtOH, was applied topically on a daily basis for four weeks. Cytokine measurements in the spleen cells (2 × 106 mL−1) were cultured for 48 hrs in a culture dish, which had been coated with CD3 mAb. Then, IL-4 and IL-13 levels were measured. The production of IL-4 and IL-13 was measured with ELISA in the culture supernatant. All measurements were made in triplicate and all values are represented as mean ± SD. Nr, normal; OVA-CT, control treated with only ovalbumin; Dexa., dexamethasone; SB, Scutellaria baicalensis Georgi extract. * p<0.05,** p<0.01, *** p<0.001 and showed a significant difference from OVA-CT.
From: Mi-Young Yun, Ju-Im Jung, Sung-Min Park, Hwa-Jung Choi,
"Enriched-Baicalein Attenuates Allergy in Cells and Mice",
Evidence-Based Complementary and Alternative Medicine, vol. 2020, 1-8, Article ID 4780210.
doi. 10.1155/2020/4780210
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone KT3 (MCA500G) used to label lymphocytes in MC38-hEpCAM tumors by immunohistochemistry on frozen sections.
Image caption:
C215Fab-SEA treatment induced a rapid T-cell influx into TDLNs and enhanced T-cell infiltration into the TME. Representative immunohistochemical (IHC) CD3 staining analysis of frozen sections (n = 2–3/group/cycle) of MC38-hEpCAM tumors from the control and treatment groups 24 h after the completion of cycle 3. Scale bar, 100 µm; IHC score (upper right): 1 + = few positive cells, 1–2 + = few to moderate numbers, 2 + = moderate numbers, 2–3 + moderate to high numbers, 3 + high numbers.
From: Azulay M, Shahar M, Shany E, Elbaz E, Lifshits S, Törngren M, Friedmann A, Kramer R, Hedlund G.
Tumor-targeted superantigens produce curative tumor immunity with induction of memory and demonstrated antigen spreading.
J Transl Med. 2023 Mar 26;21(1):222.
doi: 10.1186/s12967-023-04064-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone KT3 (MCA500G) used to label T lymphocytes in murine spinal cord by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Calnexin-deficient mice exhibit no immune cell infiltration and suppressed inflammatory gene expression. (A) Immunostaining of lumbar spinal cord sections for selected immune cell infiltrates in WT and calnexin-deficient control or EAE mice. Magnification, 20×; scale bars: 50 μm.
From: Jung J, Eggleton P, Robinson A, Wang J, Gutowski N, Holley J, Newcombe J, Dudek E, Paul AM, Zochodne D, Kraus A, Power C, Agellon LB, Michalak M.
Calnexin is necessary for T cell transmigration into the central nervous system.
JCI Insight. 2018 Mar 8;3(5):e98410.
doi: 10.1172/jci.insight.98410.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone KT3 (MCA500G) used to assess the level of T cell infiltration of gromeruli by immunohistochemistry on formalin fixed, paraffin embedded murine renal tissue sections
Image caption:
GDF15 reduces glomerular immune cell infiltration in PAN-induced kidney injury in mice. (A): Kidney sections from control and Gdf15-deficient mice were stained for (C) CD3+ T cells. The number of infiltrating cells was assessed in at least 20 glomeruli per kidney (n = 10 animals per group). Data are shown as box–whiskers plots ± SD. * p <0.05, ** p <0.01.
From: von Rauchhaupt, E.; Klaus, M.; Ribeiro, A.; Honarpisheh, M.; Li, C.; Liu, M.; Köhler, P.; Adamowicz, K.; Schmaderer, C.; Lindenmeyer, M.; et al.
GDF-15 Suppresses Puromycin Aminonucleoside-Induced Podocyte Injury by Reducing Endoplasmic Reticulum Stress and Glomerular Inflammation.
Cells 2024, 13, 637.
doi:10.3390/cells13070637.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone KT3 (MCA500G) used to detect CD3 expressing cells in murine spinal cord sections by immunofluorescence.
Image caption:
Disruption of the blood spinal cord barrier (BSCB) by low intensity pulsed ultrasound (US) enhances CNS lymphocyte infiltration in ALS mice. (a–l) Images of spinal cord sections of symptomatic stage ALS mice in the four different groups, immunostained for the lymphocyte pan-T CD3 (a,d,g,j, green) and specific lymphocyte T CD4 (b,e,h,k, red) markers and merged (c,f,i,l). Scale bars: 50 μm in (a) for (a–l) and 20 μm in inset of (a) for insets in (a–l). (m,n) Number of lymphocytes in the lumbar spinal cords of the different groups at the symptomatic stage (left graphs) or at disease end stage (right graphs), measured by counting the total number of CD3+ lymphocytes (m) or CD4+ cells (n) per spinal cord section (symptomatic, control n = 3, US n = 4, IGF1 n = 4, US IGF1 n = 5, end stage, control n = 6, US n = 5, IGF1 n = 4, US IGF1 n = 5). Bars represent mean with 95% CI. p values were determined by ANOVA followed by a post hoc Šídák multiple comparison test. (o–q) Images of a spinal cord section of a symptomatic stage ALS mouse that underwent US treatment, immunostained for the lymphocyte pan-T CD3 (o,q, green) and the cell proliferation marker Ki67 (p,q, red). Note that lymphocytes (arrowheads) are not Ki67 positive (arrows). Scale bar: 50 μm in (o) for (o–q).
From: Montero AS, Aliouat I, Ribon M, Canney M, Goldwirt L, Mourah S, Berriat F, Lobsiger CS, Pradat PF, Salachas F, Bruneteau G, Carpentier A, Boillée S.
Effect of ultrasound-mediated blood-spinal cord barrier opening on survival and motor function in females in an amyotrophic lateral sclerosis mouse model.
EBioMedicine. 2024 Jul 11;106:105235.
doi: 10.1016/j.ebiom.2024.105235.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD3:Pacific Blue®
- Product Type
- Monoclonal Antibody
- Clone
- KT3
- Isotype
- IgG2a
- Specificity
- CD3
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988). NB. For optimal staining incubations should be performed at room temperature. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- CBAT6 thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized SD rats were fused with cells of the NS0 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Pacific Blue® 410 455 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Pacific Blue® | MCA1212PB | F | 100 Tests/1ml |
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| Description | Rat IgG2a Negative Control:Pacific Blue® | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Source Reference
-
Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
Immunogenetics. 28 (6): 445-51.
References for CD3 antibody
-
Tomonari, K. (1988) A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex.
Immunogenetics. 28 (6): 455-8. -
Lazarovits, A.I. et al. (1999) Mechanisms of induction of renal allograft tolerance in CD45RB-treated mice.
Kidney Int. 55: 1303-10. -
Heitmann, S. et al. (1999) Immunohistological characterization of leukocytes in the lungs of healthy mice and after bacterial intratracheal infection.
Lab Anim. 33: 288-94. -
Tarlton, J.F. et al. (2000) The role of up-regulated serine proteases and matrix metalloproteinases in the pathogenesis of a murine model of colitis.
Am J Pathol. 157: 1927-35. -
Lacroix-Lamandé, S. et al. (2002) Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection.
Infect Immun. 70 (4): 2090-9. -
Kumar, L. et al. (2002) Differential role of SLP-76 domains in T cell development and function.
Proc Natl Acad Sci U S A. 99: 884-9. -
Hare, K.J. et al. (2003) Modeling TCR signaling complex formation in positive selection.
J Immunol. 171: 2825-31. -
Erlandsson, L. et al. (2004) Impaired B-1 and B-2 B cell development and atypical splenic B cell structures in IL-7 receptor-deficient mice.
Eur J Immunol. 34: 3595-603.
View The Latest Product References
-
Hirsh, M. et al. (2004) Response of lung gammadelta T cells to experimental sepsis in mice.
Immunology. 112: 153-60. -
Severinová, J. et al. (2005) Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.
Folia Microbiol (Praha). 50: 457-63. -
Bauer, D. et al. (2009) Amniotic membrane transplantation induces apoptosis in T lymphocytes in murine corneas with experimental herpetic stromal keratitis
Invest Ophthalmol Vis Sci. 50: 3188-98. -
Haroon, F. et al., (2011) Gp130-dependent astrocytic survival is critical for the control of autoimmune central nervous system inflammation.
J Immunol. 186: 6521-31. -
Rothhammer, V. et al. (2011) Th17 lymphocytes traffic to the central nervous system independently of α4 integrin expression during EAE.
J Exp Med. 208 (12): 2465-76. -
Salem, M. et al. (2011) Interferon regulatory factor-7 modulates experimental autoimmune encephalomyelitis in mice.
J Neuroinflammation. 8: 181. -
Teeling, J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
Acta Neuropathol. 124 (4): 479-90. -
Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
EMBO Mol Med. pii: e201404170. -
Scheinert, R.B. et al. (2016) Therapeutic effects of stress-programmed lymphocytes transferred to chronically stressed mice.
Prog Neuropsychopharmacol Biol Psychiatry. 70: 1-7. -
Janssen, E. et al. (2016) A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton.
J Clin Invest. 126 (10): 3837-51. -
Van Aelst, L.N. et al. (2016) RNA Profiling in Human and Murine Transplanted Hearts: Identification and Validation of Therapeutic Targets for Acute Cardiac and Renal Allograft Rejection.
Am J Transplant. 16 (1): 99-110. -
Kim, I. et al. (2016) Immunological characterization of de novo and recall alloantibody suppression by CTLA4Ig in a mouse model of allosensitization.
Transpl Immunol. 38: 84-92. -
Massa, M.G. et al. (2017) Testosterone Differentially Affects T Cells and Neurons in Murine and Human Models of Neuroinflammation and Neurodegeneration.
Am J Pathol. 187 (7): 1613-22. -
Granadillo, M. et al. (2019) Impact on antitumor response using a new adjuvant preparation as a component of a human papillomavirus type 16 therapeutic vaccine candidate.
Vaccine. 37 (30): 3957-60. -
Yun, M. et al. (2020) Enriched-Baicalein Attenuates Allergy in Cells and Mice
Ev-Based Comp Alt Med.. 2020: 1-8. -
Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
J Neuroinflammation. 17 (1): 283. -
Azulay, M. et al. (2023) Tumor-targeted superantigens produce curative tumor immunity with induction of memory and demonstrated antigen spreading.
J Transl Med. 21 (1): 222. -
Aloui, A. et al. (2023) AFM1 Exposure in Male Balb/c Mice and Intervention Strategies Against Its Immuno-physiological toxicity using Clay Mineral and Lactic Acid Bacteria Alone or in Combination.
Immunopharmacol Immunotoxicol. : 1-32. -
Stein, S. et al. (2021) Deletion of fibroblast activation protein provides atheroprotection.
Cardiovasc Res. 117 (4): 1060-9. -
Jung, J. et al. (2018) Calnexin is necessary for T cell transmigration into the central nervous system.
JCI Insight. 3 (5): e98410. -
Griffiths, M.R. et al. (2018) CD93 regulates central nervous system inflammation in two mouse models of autoimmune encephalomyelitis.
Immunology. 155 (3): 346-55. -
von Rauchhaupt, E. et al. (2024) GDF-15 Suppresses Puromycin Aminonucleoside-Induced Podocyte Injury by Reducing Endoplasmic Reticulum Stress and Glomerular Inflammation
Cells. 13 (7): 637. -
Montero, A.S. et al. (2024) Effect of ultrasound-mediated blood-spinal cord barrier opening on survival and motor function in females in an amyotrophic lateral sclerosis mouse model.
EBioMedicine. 106: 105235.
- UniProt
- P22646
- P24161
- P11942
- P29020
- P04235
- Entrez Gene
- Cd3d
- Cd3g
- Cd3e
- Cd247
- Cd247
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0006461 protein complex assembly
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0042105 alpha-beta T cell receptor complex
- GO:0045059 positive thymic T cell selection
- GO:0007163 establishment or maintenance of cell polarity
- GO:0015031 protein transport
- GO:0042981 regulation of apoptosis
- View More GO Terms
- GO:0046982 protein heterodimerization activity
- GO:0001772 immunological synapse
- GO:0002669 positive regulation of T cell anergy
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009897 external side of plasma membrane
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032753 positive regulation of interleukin-4 production
- GO:0045060 negative thymic T cell selection
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045879 negative regulation of smoothened signaling pathway
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050852 T cell receptor signaling pathway
- GO:0005515 protein binding
MCA500PB
1611
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