CD19/CD3 | 6D5/KT3

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Rat anti Mouse CD19:FITC/CD3:RPE

Product Type: Dual Color Reagent
Clone: 6D5/KT3
Isotype: Cocktail

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
DC035 Datasheet	F SDS	100 Tests	Log in		Get Quote

Rat anti Mouse CD19:FITC/CD3:RPE dual color reagent is a dual colour reagent recognising mouse CD19 and CD3.

Clone 6D5 recognises murine CD19, a ~95 kDa cell surface glycoprotein expressed by B lymphocytes.

Clone KT3 recognises murine CD3, a cell surface glycoprotein expressed by mature T cells.

Product Details

Target Species

Mouse

Product Form

Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

Reconstitution

Reconstitute with 1 ml distilled water

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Antibody Isotypes

FITC reagent: IgG2a (RAT)
RPE reagent: IgG2a (RAT)

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P11942; P29020; P24161; P25918; P22646; P04235
Entrez Gene: Cd3d; Cd3g; Cd3e; Cd247; Cd19; Cd247
GO Terms: GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0006461 protein complex assembly; GO:0007166 cell surface receptor linked signaling pathway; GO:0042105 alpha-beta T cell receptor complex; GO:0045059 positive thymic T cell selection; GO:0007163 establishment or maintenance of cell polarity; GO:0015031 protein transport; GO:0042981 regulation of apoptosis; GO:0046982 protein heterodimerization activity; GO:0001772 immunological synapse; GO:0002669 positive regulation of T cell anergy; GO:0008624 induction of apoptosis by extracellular signals; GO:0009897 external side of plasma membrane; GO:0032729 positive regulation of interferon-gamma production; GO:0032753 positive regulation of interleukin-4 production; GO:0045060 negative thymic T cell selection; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045879 negative regulation of smoothened signaling pathway; GO:0046641 positive regulation of alpha-beta T cell proliferation; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050850 positive regulation of calcium-mediated signaling; GO:0050852 T cell receptor signaling pathway; GO:0005515 protein binding; GO:0050853 B cell receptor signaling pathway
Regulatory: For research purposes only

Applications of CD19/CD3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rat IgG2a:FITC/Rat IgG2a:RPE Negative Control	DC036	F	100 Tests	Log in		Get Quote

Product Specific References

References for CD19/CD3

Tomonari, K. (1988) A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex.
Immunogenetics. 28 (6): 455-8.
Stables, M.J. et al. (2010) Priming innate immune responses to infection by cyclooxygenase inhibition kills antibiotic-susceptible and -resistant bacteria.
Blood. 116 (16): 2950-9.
Lis, M. et al. (2013) Modulatory effects of bestatin on T and B lymphocyte subsets and the concentration of cytokines released by Th1/Th2 lymphocytes in cyclophosphamide-treated mice
Centr Eur J Immunol 38: 42-53
Suszko, A. & Obmińska-Mrukowicz B (2013) Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate.
J Ethnopharmacol. 145 (1): 109-17.
Drynda, A. et al. (2016) Immunoregulatory effects of 4-(4-chlorophenyl)-1-(5-amino-3-methylisoxazole-4-carbonyl)-thiosemicarbazide (06K) in non-immunized and SRBC-immunized mice.
J Pharm Pharmacol. Oct 26. [Epub ahead of print]
Zimecki, M. et al. (2015) Immune function in cyclophosphamide-treated mice is restored by the T-cell-tropic isoxazole derivative R-13.
J Immunotoxicol. 12 (4): 322-9.

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