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CD19/CD3 | 6D5/KT3

Rat anti Mouse CD19:FITC/CD3:RPE

Product Type
Dual Color Reagent

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Rat anti Mouse CD19:FITC/CD3:RPE dual color reagent is a dual colour reagent recognising mouse CD19 and CD3.

Clone 6D5 recognises murine CD19, a ~95 kDa cell surface glycoprotein expressed by B lymphocytes.

Clone KT3 recognises murine CD3, a cell surface glycoprotein expressed by mature T cells.

Target Species
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitute with 1 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Antibody Isotypes
FITC reagent: IgG2a (RAT)
RPE reagent: IgG2a (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
For research purposes only
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.


This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

References for CD19/CD3

  1. Tomonari, K. (1988) A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex.
    Immunogenetics. 28 (6): 455-8.
  2. Stables, M.J. et al. (2010) Priming innate immune responses to infection by cyclooxygenase inhibition kills antibiotic-susceptible and -resistant bacteria.
    Blood. 116 (16): 2950-9.
  3. Suszko, A. & Obmińska-Mrukowicz B (2013) Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate.
    J Ethnopharmacol. 145 (1): 109-17.
  4. Drynda, A. et al. (2016) Immunoregulatory effects of 4-(4-chlorophenyl)-1-(5-amino-3-methylisoxazole-4-carbonyl)-thiosemicarbazide (06K) in non-immunized and SRBC-immunized mice.
    J Pharm Pharmacol. 68 (12): 1613-20.
  5. Zimecki, M. et al. (2015) Immune function in cyclophosphamide-treated mice is restored by the T-cell-tropic isoxazole derivative R-13.
    J Immunotoxicol. 12 (4): 322-9.
  6. Piekarska, J. et al. (2018) Effects of iridoid-anthocyanin extract of Cornus mas L. on hematological parameters, population and proliferation of lymphocytes during experimental infection of mice with Trichinella spiralis.
    Exp Parasitol. 188: 58-64.
  7. Piekarska, J. et al. (2022) Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (Lonicera caerulea L.) in the Course of Experimental Trichinellosis in Mice.
    Molecules. 27 (6): 1949.

Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0006461 protein complex assembly
GO:0007166 cell surface receptor linked signaling pathway
GO:0042105 alpha-beta T cell receptor complex
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway
GO:0005515 protein binding
GO:0050853 B cell receptor signaling pathway


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