CD152 antibody | 1B8
Hamster anti Mouse CD152
- Product Type
- Monoclonal Antibody
|Hamster anti Mouse CD152 antibody, clone 1B8 recognizes mouse CD152, also known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation.
In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis.
Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumor immunity.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Extracellular portion of mouse CD152 fused to a mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph cells from immunized Armenian hamsters were fused with cells of the murine SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD152 antibody
Walunas, T. L. et al. (1994) CTLA-4 can function as a negative regulator of T cell activation.
Immunity 1: 405-413.
Verhagen, J. et al. (2013) CTLA-4 controls the thymic development of both conventional and regulatory T cells through modulation of the TCR repertoire.
Proc Natl Acad Sci U S A. 110 (3): E221-30.
Karandikar, N.J. et al. (1996) CTLA-4: a negative regulator of autoimmune disease.
J Exp Med. 184 (2): 783-8.
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