CD152 antibody | 1B8
No associated image currently available
Hamster anti Mouse CD152
- Product Type
- Monoclonal Antibody
- Clone
- 1B8
- Isotype
- IgG
- Specificity
- CD152
Antibody Quality and Performance Guarantee
| Hamster anti Mouse CD152 antibody, clone 1B8 recognizes mouse CD152, also known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation. In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis. Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumor immunity. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Extracellular portion of mouse CD152 fused to a mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph cells from immunized Armenian hamsters were fused with cells of the murine SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/10 | 1/100 |
| Immunofluorescence | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Hamster (Armenian) IgG Negative Control | ||||||
References for CD152 antibody
-
Walunas, T. L. et al. (1994) CTLA-4 can function as a negative regulator of T cell activation.
Immunity 1: 405-413. -
Verhagen, J. et al. (2013) CTLA-4 controls the thymic development of both conventional and regulatory T cells through modulation of the TCR repertoire.
Proc Natl Acad Sci U S A. 110 (3): E221-30.
Further Reading
-
Karandikar, N.J. et al. (1996) CTLA-4: a negative regulator of autoimmune disease.
J Exp Med. 184 (2): 783-8.
- Synonyms
- CTLA-4
- RRID
- AB_2087650
- UniProt
- P09793
- Entrez Gene
- Ctla4
- GO Terms
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
MCA4636GA
148805
152471
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD152 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up