CD11c antibody | N418

Hamster anti Mouse CD11c

Product Type
Monoclonal Antibody
Clone
N418
Isotype
IgG
Specificity
CD11c

Product Code Applications Pack Size List Price Your Price Qty
MCA1369
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 0.25 mg loader
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loader
MCA1369GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 0.1 mg loader
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loader
MCA1369T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 25 µg loader
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loader
Search for Batch Specific Datasheets

Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, also known as Integrin Alpha X, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells.

Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
MCA1369: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1369GA, MCA1369T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Mouse spleen dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
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Description Goat F(ab')2 anti Hamster IgG:FITC
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Description Goat anti Hamster IgG:Biotin
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP
Goat F(ab')2 anti Hamster IgG:DyLight®550 STAR104D550 F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550
Goat F(ab')2 anti Hamster IgG:DyLight®650 STAR104D650 F IF 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®650

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

Source Reference

  1. Metlay, J.P. et al. (1990) The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.
    J Exp Med. 171 (5): 1753-71.

References for CD11c antibody

  1. Crowley, M.T. et al. (1990) Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
    J Immunol Methods. 133 (1): 55-66.
  2. Dahlen, E. et al. (1998) Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse.
    J Immunol. 160: 3585-93.
  3. Wang, H. et al. (2000) Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization.
    Proc Natl Acad Sci U S A. 97 (2): 847-52.
  4. Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
    Infect Immun. 78 (5): 1924-30.
  5. Beyer, M. et al. (2005) The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
    Respir Res. 6: 70.
  6. Goupil, M. et al. (2009) Macrophage-mediated responses to Candida albicans in mice expressing the human immunodeficiency virus type 1 transgene.
    Infect Immun. 77: 4136-49.
  7. Linehan, S.A.. (2005) The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
    BMC Immunol. 6:4.
  8. Bjorck, P. (2004) Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity.
    J Immunol. 172: 5396-404.
  9. View The Latest Product References
  10. Mercier, S. et al. (2002) Distinct roles of adenovirus vector-transduced dendritic cells, myoblasts, and endothelial cells in mediating an immune response against a transgene product.
    J Virol. 76: 2899-911.
  11. de Jersey, J. et al. (2002) Activation of CD8 T cells by antigen expressed in the pituitary gland.
    J Immunol. 169: 6753-9.
  12. Dimier-Poisson, I. et al. (2003) Protective mucosal Th2 immune response against Toxoplasma gondii by murine mesenteric lymph node dendritic cells.
    Infect Immun. 71: 5254-65.
  13. Gonzalez-Juarrero, M. and Orme, I.M. (2001) Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis.
    Infect Immun. 69: 1127-33.
  14. Hamada, H. et al. (2002) Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine.
    J Immunol. 168: 57-64.
  15. Meng, Q. et al. (2007) Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
    Mol Vis. 13: 475-86.
  16. Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice
    Arterioscler Thromb Vasc Biol. 25: 2386-91.
  17. Nunez, R. et al. (1999) Immortalized cell lines derived from mice lacking both type I and type II IFN receptors unify some functions of immature and mature dendritic cells.
    Immunol Cell Biol. 77: 153-63.
  18. Ponce, L.V. et al. (2005) Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
    Kinetoplastid Biol Dis. 4: 2.
  19. Zhang, L. et al. (2011) The inflammatory changes of adipose tissue in late pregnant mice.
    J Mol Endocrinol. 47 (2): 157-65.
  20. Donaldson, D.S. et al. (2012) M cell-depletion blocks oral prion disease pathogenesis.
    Mucosal Immunol. 5: 216-25.
  21. Wada, T. et al. (2013) Eplerenone ameliorates the phenotypes of metabolic syndrome with NASH in liver-specific SREBP-1c Tg mice fed high-fat and high-fructose diet.
    Am J Physiol Endocrinol Metab. 305 (11): E1415-25.
  22. Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
    J Neurosci. 35 (15): 5969-82.
  23. Kayser, B.D. et al. (2015) Perinatal Overnutrition Exacerbates Adipose Tissue Inflammation Caused by High-Fat Feeding in C57BL/6J Mice.
    PLoS One. 10 (3): e0121954.
  24. Sehgal, A. et al. (2017) c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.
    Immunobiology. 222 (2): 316-26.
  25. Bender, L.H. et al. (2020) Intratumoral Administration of a Novel Cytotoxic Formulation with Strong Tissue Dispersive Properties Regresses Tumor Growth and Elicits Systemic Adaptive Immunity in In Vivo Models.
    Int J Mol Sci. 21 (12) Jun 24 [Epub ahead of print].
  26. Iida, Y. et al. (2020) Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells.
    J Immunother Cancer. 8 (2) [Epub ahead of print].
  27. Kouwenberg, M. et al. (2021) Reduced CXCL1 production by endogenous IL-37 expressing dendritic cells does not affect T cell activation.
    PLoS One. 16 (5): e0251809.
  28. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.
  29. Mohanta, S.K. et al. (2022) Neuroimmune cardiovascular interfaces control atherosclerosis.
    Nature. Apr 27 [Epub ahead of print].

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Synonyms
Integrin Alpha X Chain
RRID
AB_324490
UniProt
Q9QXH4
Entrez Gene
Itgax
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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