CD11c antibody | N418
Hamster anti Mouse CD11c antibody, clone N418 used for the detection of CD11c expressing cells in mouse spleen by immunofluorescence.
Image caption:
Mannose receptor positive and negative APC subsets identified by immunofluorescence microscopy. Tissues were examined by immunofluorescence microscopy for MR expression and other APC phenotypic markers in dual-labeled specimens as indicated. MR was detected using MR6F3A488 mAb. Images of each antigen labelling are shown separately in gray-scale, and merged in colour, in some cases with nuclear counterstaining shown in blue. Colocalisation of red and green is indicated by yellow. Examples of MHC class II-positive APC which coexppress MR are indicated with arrows. These constitute a subset of APC in the skin, liver, tongue and heart. No background was detected with an irrelevant IgG2aA488 negative control (shown in the spleen inset of MR-labeling only). Background labeling due to secondary Abs alone was also not detected in control sections (shown for anti-hamster IgG in the spleen inset of CD11c labeling only). Scale bars are indicated in MR-labeled panels only, and are 100μm.
From: Linehan SA. The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 2005 Feb 8;6:4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 used for the detection of CD11c expressing cells in mouse spleen by immunofluorescence.
Image caption:
Mannose receptor positive and negative APC subsets identified by immunofluorescence microscopy. Tissues were examined by immunofluorescence microscopy for MR expression and other APC phenotypic markers in dual-labeled specimens as indicated. MR was detected using MR6F3A488 mAb. Images of each antigen labelling are shown separately in gray-scale, and merged in colour, in some cases with nuclear counterstaining shown in blue. Colocalisation of red and green is indicated by yellow. Examples of MHC class II-positive APC which coexppress MR are indicated with arrows. These constitute a subset of APC in the skin, liver, tongue and heart. No background was detected with an irrelevant IgG2aA488 negative control (shown in the spleen inset of MR-labeling only). Background labeling due to secondary Abs alone was also not detected in control sections (shown for anti-hamster IgG in the spleen inset of CD11c labeling only). Scale bars are indicated in MR-labeled panels only, and are 100μm.
From: Linehan SA. The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 2005 Feb 8;6:4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 used for the detection of CD11c expressing cells in murine adipose tissue by immunofluorescence.
Image caption:
The definition of macrophage subtypes in adipose tissue from non-pregnant and pregnant mice. (A) Identification of F4/80+ CD11c+ macrophages by immunofluorescence microscopy in the frozen sections of SAT stained with antibodies against F4/80 (left) and CD11c (middle) and imaged by confocal microscopy to identify surface markers to confirm the presence of CD11c+ cells. F4/80+ CD11c+ macrophage: M1 macrophage; F4/80− CD11c+ macrophage: M2 macrophage (n=6 per group). (B) The ratio of M1–M2 macrophages in SAT (n=6 per group). (C) Identification of F4/80+ and CD11c+ macrophages in PAT by immunofluorescence microscopy (n=6 per group). (D) The ratio of M1–M2 macrophages in PAT (n=6 per group). Data are presented as the mean±s.e.m. A two-tail Student's t-test was performed. P versus NP: ***P<0.001.
From: Zhang L, Sugiyama T, Murabayashi N, Umekawa T, Ma N, Kamimoto Y, Ogawa Y, Sagawa N.
The inflammatory changes of adipose tissue in late pregnant mice.
J Mol Endocrinol. 2011 Aug 30;47(2):157-65.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) used for the evaluation of CD11c expression on dendritic cells by flow cytometry
Image caption:
Frequency distributions of purified dendritic cells labeled CD11c-FITC showing 97.17% purity (right), and FITC-isotype control (IgG1) (left). The information shown is from a single cell isolation procedure, representative of various separate experiments.
From: Ponce LV, Corado J, Díaz NL, Tapia FJ. Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
Kinetoplastid Biol Dis. 2005 Jan 25;4:2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) used for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.
Image caption:
Expression of CD11c on pulmonary CD8+ T cells following RSV infection. Following primary RSV infection lungs, spleens, MLN and blood were harvested from BALB/c mice at the time points indicated. Cells were stained with antibodies to CD3, CD8, and CD11c, CD11b or F4/80. Dot plots show expression of CD11c and CD8 on cells gated for CD3+ lymphocytes harvested from A) lungs, B) spleen, MLN and blood. Numbers indicate the percentage of CD8+ cells. Quadrant settings were adjusted that less than 1% of CD8+ cells stained positive for the isotype control of anti-CD11c-mAb. C) Changes in expression of CD11c, CD11b and F4/80 on CD3+ CD8+ cells from lungs, spleen, MLN, and blood following RSV infection. Cells were stained with antibodies as indicated and analyzed by FACS. The percentage of CD3+8+ cells staining positive for CD11c (black squares), CD11b (open circles) or F4/80 (triangles) after subtraction of isotype controls is shown as mean ± SEM (n = 6, *p < 0.05, significant difference versus dpi 0).
From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 2005 Jul 12;6:70.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.
Image caption:
Differential expression of surface markers on pulmonary CD11c+ and CD11c- CD8+ T cells. Lungs of naive mice or on day 8 after RSV infection were harvested. Cells were stained with antibodies for CD8, CD11c, and the surface markers indicated. A) Dot plots show expression of CD11c versus the indicated marker on cells gated for lymphocyte size and CD8 expression after RSV infection. Numbers indicate the percentage of cells in corresponding quadrants. Quadrants were set according to isotype controls except for CD11a, CD54 and CD62L. Since these three molecules are expressed to a considerable degree on naïve CD8 T cells, this expression was used for quadrant settings to highlight differences in CD11+ CTL. B) Histograms show expression of CD54, CD62L, and CD69 on pulmonary CD8+ cells from naïve (dotted line) or RSV infected mice (CD11c+ cells: black line, CD11c- cells: filled histogram). .
From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 2005 Jul 12;6:70.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369GA) used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.
Image caption:
Distribution and morphology of labeled cells in the murine cornea at 24 h after intravitreal injection of fluorophore-conjugated reagents. A decreasing density of positive cells is observed from peripheral to central cornea (A,D: peripheral cornea, B,E: paracentral cornea, C,F: central cornea. A-C: MHC-II+ cells, D-F: OVA+ cells). Two populations of each antibody labeled cells, i.e., dendriform (G: MHC-II+ cell, I: CD11c+ cells) and round or irregular shapes (H: MHC-II+ cell, I: CD11c+ cells, J: F4/80+ cell, K: B7-1+ cell) are identified. F4/80+ cells seem to be more elongated in the peripheral area (L), slightly ramose in the paracentral region (M), and more round or oblong in the central cornea (N). All OVA+ cells show round, oblong or irregular shapes (O).
From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR. Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 2007 Mar28;13:475-86.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.
Image caption:
Phenotypes of APCs in the normal cornea shown in confocal microscopy images. Corneas are double stained in vivo with OVA and antibodies, followed by examination of wholemounts under the confocal microscopy. A number of F4/80+ (A: green) and CD11c+ (B: green) cells are positive for OVA (A,B: red). A few of F4/80+ cells are positively costained with anti-CD11c (C: red F4/80, green CD11c). A number of CD11c+ cells (D: green) and F4/80+ cells (E: red) are also MHC-II positive (D: red, E: green). More than half of CD11c+ cells are double stained for B7-2 (F: red CD11c, green B7-2). Double-labeled cells are yellow.
From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR. Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 2007 Mar28;13:475-86.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD11c:Alexa Fluor® 488
Hamster anti Mouse CD11c:Alexa Fluor® 647
Hamster anti Mouse CD11c:Biotin
Hamster anti Mouse CD11c:FITC
Hamster anti Mouse CD11c
Hamster anti Mouse CD11c:RPE
Hamster anti Mouse CD11c:RPE-Alexa Fluor® 647
Hamster anti Mouse CD11c:RPE-Alexa Fluor® 750
- Product Type
- Monoclonal Antibody
- Clone
- N418
- Isotype
- IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1369A488T | F | 25 Tests/0.25ml |
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| MCA1369A488 | F | 100 Tests/1ml |
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| MCA1369A647T | F | 25 Tests/0.25ml |
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| MCA1369A647 | F | 100 Tests/1ml |
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| MCA1369BT | F | 25 µg |
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| MCA1369B | F | 0.1 mg |
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| MCA1369F | F | 0.1 mg |
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| MCA1369T | C F IF IP | 25 µg |
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| MCA1369GA | C F IF IP | 0.1 mg |
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| MCA1369 | C F IF IP | 0.25 mg |
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| MCA1369PE | F | 100 Tests |
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| MCA1369P647 | F | 100 Tests/1ml |
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| MCA1369P750 | F | 100 Tests/1ml |
|
Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, also known as Integrin Alpha X, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells.
Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).
Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Mouse spleen dendritic cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- Q9QXH4
- Entrez Gene
- Itgax
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009897 external side of plasma membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD11c antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/100 | |
| Flow Cytometry | 1/10 | 1/100 | |
| Flow Cytometry | 1/5 Pack Size: 25 µg neat Pack Size: 0.1 mg |
1/10 Pack Size: 25 µg 1/5 Pack Size: 0.1 mg |
|
| Flow Cytometry | 1/100 | ||
| Flow Cytometry | 1/25 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
|
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| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
|
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| Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
|
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| Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
|
|
| Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 | MCA2356A488 | F | 100 Tests/1ml |
|
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| Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 | MCA2356A647 | F | 100 Tests/1ml |
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| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | F | 0.1 mg |
|
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| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
|
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| Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | F | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for CD11c antibody
-
Crowley, M.T. et al. (1990) Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
J Immunol Methods. 133 (1): 55-66. -
Metlay, J.P. et al. (1990) The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.
J Exp Med. 171 (5): 1753-71. -
Wang, H. et al. (2000) Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization.
Proc Natl Acad Sci U S A. 97 (2): 847-52. -
Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
Infect Immun. 78 (5): 1924-30. -
Beyer, M. et al. (2005) The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 6: 70. -
Goupil, M. et al. (2009) Macrophage-mediated responses to Candida albicans in mice expressing the human immunodeficiency virus type 1 transgene.
Infect Immun. 77: 4136-49. -
Linehan, S.A.. (2005) The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 6:4. -
Bjorck, P. (2004) Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity.
J Immunol. 172: 5396-404. -
Dahlen, E. et al. (1998) Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse.
J Immunol. 160: 3585-93. -
de Jersey, J. et al. (2002) Activation of CD8 T cells by antigen expressed in the pituitary gland.
J Immunol. 169: 6753-9. -
Dimier-Poisson, I. et al. (2003) Protective mucosal Th2 immune response against Toxoplasma gondii by murine mesenteric lymph node dendritic cells.
Infect Immun. 71: 5254-65. -
Gonzalez-Juarrero, M. and Orme, I.M. (2001) Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis.
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