CD11c antibody | N418
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369GA) used for the detection of CD11c expressing cells in mouse spleen by immunofluorescence.
Image caption:
Mannose receptor positive and negative APC subsets identified by immunofluorescence microscopy. Tissues were examined by immunofluorescence microscopy for MR expression and other APC phenotypic markers in dual-labeled specimens as indicated. MR was detected using MR6F3A488 mAb. Images of each antigen labelling are shown separately in gray-scale, and merged in colour, in some cases with nuclear counterstaining shown in blue. Colocalisation of red and green is indicated by yellow. Examples of MHC class II-positive APC which coexppress MR are indicated with arrows. These constitute a subset of APC in the skin, liver, tongue and heart. No background was detected with an irrelevant IgG2aA488 negative control (shown in the spleen inset of MR-labeling only). Background labeling due to secondary Abs alone was also not detected in control sections (shown for anti-hamster IgG in the spleen inset of CD11c labeling only). Scale bars are indicated in MR-labeled panels only, and are 100μm.
From: Linehan SA. The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 2005 Feb 8;6:4.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) used for the detection of CD11c expressing cells in murine adipose tissue by immunofluorescence.
Image caption:
The definition of macrophage subtypes in adipose tissue from non-pregnant and pregnant mice. (A) Identification of F4/80+ CD11c+ macrophages by immunofluorescence microscopy in the frozen sections of SAT stained with antibodies against F4/80 (left) and CD11c (middle) and imaged by confocal microscopy to identify surface markers to confirm the presence of CD11c+ cells. F4/80+ CD11c+ macrophage: M1 macrophage; F4/80− CD11c+ macrophage: M2 macrophage (n=6 per group). (B) The ratio of M1–M2 macrophages in SAT (n=6 per group). (C) Identification of F4/80+ and CD11c+ macrophages in PAT by immunofluorescence microscopy (n=6 per group). (D) The ratio of M1–M2 macrophages in PAT (n=6 per group). Data are presented as the mean±s.e.m. A two-tail Student's t-test was performed. P versus NP: ***P<0.001.
From: Zhang L, Sugiyama T, Murabayashi N, Umekawa T, Ma N, Kamimoto Y, Ogawa Y, Sagawa N.
The inflammatory changes of adipose tissue in late pregnant mice.
J Mol Endocrinol. 2011 Aug 30;47(2):157-65.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) used for the evaluation of CD11c expression on dendritic cells by flow cytometry
Image caption:
Frequency distributions of purified dendritic cells labeled CD11c-FITC showing 97.17% purity (right), and FITC-isotype control (IgG1) (left). The information shown is from a single cell isolation procedure, representative of various separate experiments.
From: Ponce LV, Corado J, Díaz NL, Tapia FJ.
Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
Kinetoplastid Biol Dis. 2005 Jan 25;4:2.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) used for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.
Image caption:
Expression of CD11c on pulmonary CD8+ T cells following RSV infection. Following primary RSV infection lungs, spleens, MLN and blood were harvested from BALB/c mice at the time points indicated. Cells were stained with antibodies to CD3, CD8, and CD11c, CD11b or F4/80. Dot plots show expression of CD11c and CD8 on cells gated for CD3+ lymphocytes harvested from A) lungs, B) spleen, MLN and blood. Numbers indicate the percentage of CD8+ cells. Quadrant settings were adjusted that less than 1% of CD8+ cells stained positive for the isotype control of anti-CD11c-mAb. C) Changes in expression of CD11c, CD11b and F4/80 on CD3+ CD8+ cells from lungs, spleen, MLN, and blood following RSV infection. Cells were stained with antibodies as indicated and analyzed by FACS. The percentage of CD3+8+ cells staining positive for CD11c (black squares), CD11b (open circles) or F4/80 (triangles) after subtraction of isotype controls is shown as mean ± SEM (n = 6, *p < 0.05, significant difference versus dpi 0).
From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 2005 Jul 12;6:70.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.
Image caption:
Differential expression of surface markers on pulmonary CD11c+ and CD11c- CD8+ T cells. Lungs of naive mice or on day 8 after RSV infection were harvested. Cells were stained with antibodies for CD8, CD11c, and the surface markers indicated. A) Dot plots show expression of CD11c versus the indicated marker on cells gated for lymphocyte size and CD8 expression after RSV infection. Numbers indicate the percentage of cells in corresponding quadrants. Quadrants were set according to isotype controls except for CD11a, CD54 and CD62L. Since these three molecules are expressed to a considerable degree on naïve CD8 T cells, this expression was used for quadrant settings to highlight differences in CD11+ CTL. B) Histograms show expression of CD54, CD62L, and CD69 on pulmonary CD8+ cells from naïve (dotted line) or RSV infected mice (CD11c+ cells: black line, CD11c- cells: filled histogram). .
From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J.
The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 2005 Jul 12;6:70.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369GA) used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.
Image caption:
Distribution and morphology of labeled cells in the murine cornea at 24 h after intravitreal injection of fluorophore-conjugated reagents. A decreasing density of positive cells is observed from peripheral to central cornea (A,D: peripheral cornea, B,E: paracentral cornea, C,F: central cornea. A-C: MHC-II+ cells, D-F: OVA+ cells). Two populations of each antibody labeled cells, i.e., dendriform (G: MHC-II+ cell, I: CD11c+ cells) and round or irregular shapes (H: MHC-II+ cell, I: CD11c+ cells, J: F4/80+ cell, K: B7-1+ cell) are identified. F4/80+ cells seem to be more elongated in the peripheral area (L), slightly ramose in the paracentral region (M), and more round or oblong in the central cornea (N). All OVA+ cells show round, oblong or irregular shapes (O).
From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR.
Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 2007 Mar28;13:475-86.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.
Image caption:
Phenotypes of APCs in the normal cornea shown in confocal microscopy images. Corneas are double stained in vivo with OVA and antibodies, followed by examination of wholemounts under the confocal microscopy. A number of F4/80+ (A: green) and CD11c+ (B: green) cells are positive for OVA (A,B: red). A few of F4/80+ cells are positively costained with anti-CD11c (C: red F4/80, green CD11c). A number of CD11c+ cells (D: green) and F4/80+ cells (E: red) are also MHC-II positive (D: red, E: green). More than half of CD11c+ cells are double stained for B7-2 (F: red CD11c, green B7-2). Double-labeled cells are yellow.
From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR.
Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 2007 Mar28;13:475-86.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369) used for the detection of CD11c expressing cells in mouse spleen by immunofluorescence.
Image caption:
Mannose receptor positive and negative APC subsets identified by immunofluorescence microscopy. Tissues were examined by immunofluorescence microscopy for MR expression and other APC phenotypic markers in dual-labeled specimens as indicated. MR was detected using MR6F3A488 mAb. Images of each antigen labelling are shown separately in gray-scale, and merged in colour, in some cases with nuclear counterstaining shown in blue. Colocalisation of red and green is indicated by yellow. Examples of MHC class II-positive APC which coexppress MR are indicated with arrows. These constitute a subset of APC in the skin, liver, tongue and heart. No background was detected with an irrelevant IgG2aA488 negative control (shown in the spleen inset of MR-labeling only). Background labeling due to secondary Abs alone was also not detected in control sections (shown for anti-hamster IgG in the spleen inset of CD11c labeling only). Scale bars are indicated in MR-labeled panels only, and are 100μm.
From: Linehan SA. The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 2005 Feb 8;6:4.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369GA) used for the identification of dendritic cells in tumor tissues by immunohistochemistry on formalin fixed cryosections. Comparison is with the distribution of F4/80+ macrophages (MCA497R) in similar tissue sections.
Image caption:
IHC analysis of Colon-26 tumors untreated and INT230-6-treated tumors ~10 days post dose. Representative images (40× magnification) of IHC staining for F4/80 (macrophages) and CD11c (dendritic cells). In general, the staining appeared more evident in the INT230-6-treated tumors, particularly toward the more necrotic areas.
From: Bender LH, Abbate F, Walters IB.
Intratumoral Administration of a Novel Cytotoxic Formulation with Strong Tissue Dispersive Properties Regresses Tumor Growth and Elicits Systemic Adaptive Immunity in In Vivo Models.
Int J Mol Sci. 2020;2 (12):E4493.
doi:10.3390/ijms21124493
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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IF F C ResetHamster anti Mouse CD11c
- Product Type
- Monoclonal Antibody
- Clone
- N418
- Isotype
- IgG
- Specificity
- CD11c
Quick Links:
Antibody Quality and 
Unrivaled 
| Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, also known as Integrin Alpha X, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1369GA, MCA1369T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1369: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Mouse spleen dendritic cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Source Reference
-
Metlay, J.P. et al. (1990) The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.
J Exp Med. 171 (5): 1753-71.
References for CD11c antibody
-
Crowley, M.T. et al. (1990) Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
J Immunol Methods. 133 (1): 55-66. -
Dahlen, E. et al. (1998) Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse.
J Immunol. 160: 3585-93. -
Wang, H. et al. (2000) Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization.
Proc Natl Acad Sci U S A. 97 (2): 847-52. -
Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
Infect Immun. 78 (5): 1924-30. -
Beyer, M. et al. (2005) The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 6: 70. -
Goupil, M. et al. (2009) Macrophage-mediated responses to Candida albicans in mice expressing the human immunodeficiency virus type 1 transgene.
Infect Immun. 77: 4136-49. -
Linehan, S.A.. (2005) The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 6:4. -
Bjorck, P. (2004) Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity.
J Immunol. 172: 5396-404.
View The Latest Product References
-
Mercier, S. et al. (2002) Distinct roles of adenovirus vector-transduced dendritic cells, myoblasts, and endothelial cells in mediating an immune response against a transgene product.
J Virol. 76: 2899-911. -
de Jersey, J. et al. (2002) Activation of CD8 T cells by antigen expressed in the pituitary gland.
J Immunol. 169: 6753-9. -
Dimier-Poisson, I. et al. (2003) Protective mucosal Th2 immune response against Toxoplasma gondii by murine mesenteric lymph node dendritic cells.
Infect Immun. 71: 5254-65. -
Gonzalez-Juarrero, M. and Orme, I.M. (2001) Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis.
Infect Immun. 69: 1127-33. -
Hamada, H. et al. (2002) Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine.
J Immunol. 168: 57-64. -
Meng, Q. et al. (2007) Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 13: 475-86. -
Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice
Arterioscler Thromb Vasc Biol. 25: 2386-91. -
Nunez, R. et al. (1999) Immortalized cell lines derived from mice lacking both type I and type II IFN receptors unify some functions of immature and mature dendritic cells.
Immunol Cell Biol. 77: 153-63. -
Ponce, L.V. et al. (2005) Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
Kinetoplastid Biol Dis. 4: 2. -
Zhang, L. et al. (2011) The inflammatory changes of adipose tissue in late pregnant mice.
J Mol Endocrinol. 47 (2): 157-65. -
Donaldson, D.S. et al. (2012) M cell-depletion blocks oral prion disease pathogenesis.
Mucosal Immunol. 5: 216-25. -
Wada, T. et al. (2013) Eplerenone ameliorates the phenotypes of metabolic syndrome with NASH in liver-specific SREBP-1c Tg mice fed high-fat and high-fructose diet.
Am J Physiol Endocrinol Metab. 305 (11): E1415-25. -
Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
J Neurosci. 35 (15): 5969-82. -
Kayser, B.D. et al. (2015) Perinatal Overnutrition Exacerbates Adipose Tissue Inflammation Caused by High-Fat Feeding in C57BL/6J Mice.
PLoS One. 10 (3): e0121954. -
Sehgal, A. et al. (2017) c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.
Immunobiology. 222 (2): 316-26. -
Bender, L.H. et al. (2020) Intratumoral Administration of a Novel Cytotoxic Formulation with Strong Tissue Dispersive Properties Regresses Tumor Growth and Elicits Systemic Adaptive Immunity in In Vivo Models.
Int J Mol Sci. 21 (12) Jun 24 [Epub ahead of print]. -
Iida, Y. et al. (2020) Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells.
J Immunother Cancer. 8 (2) [Epub ahead of print]. -
Kouwenberg, M. et al. (2021) Reduced CXCL1 production by endogenous IL-37 expressing dendritic cells does not affect T cell activation.
PLoS One. 16 (5): e0251809. -
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53. -
Mohanta, S.K. et al. (2022) Neuroimmune cardiovascular interfaces control atherosclerosis.
Nature. Apr 27 [Epub ahead of print].
- Synonyms
- Integrin Alpha X Chain
- RRID
- AB_324490
- UniProt
- Q9QXH4
- Entrez Gene
- Itgax
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009897 external side of plasma membrane
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