CD11c antibody | N418










Hamster anti Mouse CD11c:Alexa Fluor® 488
Hamster anti Mouse CD11c:Alexa Fluor® 647
Hamster anti Mouse CD11c:Biotin
Hamster anti Mouse CD11c:FITC
Hamster anti Mouse CD11c
Hamster anti Mouse CD11c:RPE
Hamster anti Mouse CD11c:RPE-Alexa Fluor® 750
- Product Type
- Monoclonal Antibody
- Clone
- N418
- Isotype
- IgG
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1369A488T | F | 25 Tests/0.25ml |
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MCA1369A488 | F | 100 Tests/1ml |
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MCA1369A647T | F | 25 Tests/0.25ml |
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MCA1369A647 | F | 100 Tests/1ml |
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MCA1369BT | F | 25 µg |
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MCA1369B | F | 0.1 mg |
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MCA1369F | F | 0.1 mg |
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MCA1369T | C F IF IP | 25 µg |
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MCA1369GA | C F IF IP | 0.1 mg |
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MCA1369 | C F IF IP | 0.25 mg |
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MCA1369PE | F | 100 Tests |
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MCA1369P750 | F | 100 Tests/1ml |
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Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Mouse spleen dendritic cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9QXH4
- Entrez Gene
- Itgax
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009897 external side of plasma membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD11c antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/10 | 1/100 | |
Flow Cytometry | 1/10 | 1/100 | |
Flow Cytometry | 1/5 Pack Size: 25 µg neat Pack Size: 0.1 mg |
1/10 Pack Size: 25 µg 1/5 Pack Size: 0.1 mg |
|
Flow Cytometry | 1/100 | ||
Flow Cytometry | 1/25 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
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Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
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Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
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Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
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Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 | MCA2356A488 | F | 100 Tests/1ml |
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Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 | MCA2356A647 | F | 100 Tests/1ml |
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Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | F | 0.1 mg |
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Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | F | 100 Tests/1ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
Source Reference
-
Metlay, J.P. et al. (1990) The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.
J Exp Med. 171 (5): 1753-71.
References for CD11c antibody
-
Crowley, M.T. et al. (1990) Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
J Immunol Methods. 133 (1): 55-66. -
Dahlen, E. et al. (1998) Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse.
J Immunol. 160: 3585-93. -
Wang, H. et al. (2000) Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization.
Proc Natl Acad Sci U S A. 97 (2): 847-52. -
Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
Infect Immun. 78 (5): 1924-30. -
Beyer, M. et al. (2005) The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 6: 70. -
Goupil, M. et al. (2009) Macrophage-mediated responses to Candida albicans in mice expressing the human immunodeficiency virus type 1 transgene.
Infect Immun. 77: 4136-49. -
Linehan, S.A.. (2005) The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 6:4. -
Bjorck, P. (2004) Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity.
J Immunol. 172: 5396-404. -
Mercier, S. et al. (2002) Distinct roles of adenovirus vector-transduced dendritic cells, myoblasts, and endothelial cells in mediating an immune response against a transgene product.
J Virol. 76: 2899-911. -
de Jersey, J. et al. (2002) Activation of CD8 T cells by antigen expressed in the pituitary gland.
J Immunol. 169: 6753-9. -
Dimier-Poisson, I. et al. (2003) Protective mucosal Th2 immune response against Toxoplasma gondii by murine mesenteric lymph node dendritic cells.
Infect Immun. 71: 5254-65. -
Gonzalez-Juarrero, M. and Orme, I.M. (2001) Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis.
Infect Immun. 69: 1127-33. -
Hamada, H. et al. (2002) Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine.
J Immunol. 168: 57-64. -
Meng, Q. et al. (2007) Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 13: 475-86. -
Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice
Arterioscler Thromb Vasc Biol. 25: 2386-91. -
Nunez, R. et al. (1999) Immortalized cell lines derived from mice lacking both type I and type II IFN receptors unify some functions of immature and mature dendritic cells.
Immunol Cell Biol. 77: 153-63. -
Ponce, L.V. et al. (2005) Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
Kinetoplastid Biol Dis. 4: 2. -
Zhang, L. et al. (2011) The inflammatory changes of adipose tissue in late pregnant mice.
J Mol Endocrinol. 47 (2): 157-65. -
Donaldson, D.S. et al. (2012) M cell-depletion blocks oral prion disease pathogenesis.
Mucosal Immunol. 5: 216-25. -
Wada, T. et al. (2013) Eplerenone ameliorates the phenotypes of metabolic syndrome with NASH in liver-specific SREBP-1c Tg mice fed high-fat and high-fructose diet.
Am J Physiol Endocrinol Metab. 305 (11): E1415-25. -
Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
J Neurosci. 35 (15): 5969-82. -
Kayser, B.D. et al. (2015) Perinatal Overnutrition Exacerbates Adipose Tissue Inflammation Caused by High-Fat Feeding in C57BL/6J Mice.
PLoS One. 10 (3): e0121954. -
Sehgal, A. et al. (2017) c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.
Immunobiology. 222 (2): 316-26. -
Bender, L.H. et al. (2020) Intratumoral Administration of a Novel Cytotoxic Formulation with Strong Tissue Dispersive Properties Regresses Tumor Growth and Elicits Systemic Adaptive Immunity in In Vivo Models.
Int J Mol Sci. 21 (12) Jun 24 [Epub ahead of print]. -
Iida, Y. et al. (2020) Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells.
J Immunother Cancer. 8 (2) [Epub ahead of print].
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