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ZAP 70 antibody | SBZAP

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Mouse anti Human ZAP 70:FITC

Product Type
Monoclonal Antibody
Clone
SBZAP
Isotype
IgG1
Specificity
ZAP 70

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Product Code Applications Pack Size List Price Your Price Qty
1065002S
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 25 µg loader
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Mouse anti Human ZAP 70 antibody, clone SBZAP recognizes human ZAP-70, a 70kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia (Deglesne et al. 2006).

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 280-309 of human ZAP70 conjugated to keyhole limpet hemocyanin (KLH).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock
Leucoperm BUF09 F 50 Tests
List Price Your Price
Description Leucoperm
Leucoperm BUF09B F 250 Tests
List Price Your Price
Description Leucoperm
Leucoperm BUF09C F 1000 Tests
List Price Your Price
Description Leucoperm

References for ZAP 70 antibody

  1. Roifman, C.M. et al. (1989) Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.
    J Exp Med. 170 (6): 2177-82.
  2. Arpaia, E. et al. (1994) Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase.
    Cell. 76 (5): 947-58.

RRID
AB_616761
UniProt
P43403
Entrez Gene
ZAP70
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0006955 immune response
GO:0007243 intracellular protein kinase cascade
GO:0042101 T cell receptor complex
GO:0045059 positive thymic T cell selection
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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