ZAP 70 antibody | SBZAP
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Mouse anti Human ZAP 70 antibody, clone SBZAP recognizes human ZAP-70, a 70kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 280-309 of human ZAP70 conjugated to keyhole limpet hemocyanin (KLH).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. This product should be stored undiluted.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P43403 Related reagents
- Entrez Gene
- ZAP70 Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0005524 ATP binding
- GO:0006955 immune response
- GO:0007243 intracellular protein kinase cascade
- GO:0042101 T cell receptor complex
- GO:0045059 positive thymic T cell selection
- GO:0050852 T cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of ZAP 70 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Leucoperm™ | BUF09 | F | 50 Tests | ||
| Leucoperm™ | BUF09B | F | 250 Tests | ||
| Leucoperm™ | BUF09C | F | 1000 Tests |
Product Specific References
References for ZAP 70 antibody
-
Roifman, C.M. et al. (1989) Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.
J Exp Med. 170 (6): 2177-82. -
Arpaia, E. et al. (1994) Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase.
Cell. 76 (5): 947-58.
