ZAP 70 antibody | SBZAP

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for ZAP 70 antibody

1. Roifman, C.M. et al. (1989) Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.
   J Exp Med. 170 (6): 2177-82.
2. Arpaia, E. et al. (1994) Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase.
   Cell. 76 (5): 947-58.

RRID

AB_616761

UniProt

P43403

Entrez Gene

ZAP70

GO Terms

GO:0005829 cytosol

GO:0004715 non-membrane spanning protein tyrosine kinase activity

GO:0005524 ATP binding

GO:0006955 immune response

GO:0007243 intracellular protein kinase cascade

GO:0042101 T cell receptor complex

GO:0045059 positive thymic T cell selection

GO:0050852 T cell receptor signaling pathway

1065002S

153688 156165 157153 157551 158101 158473 159857 161108 162559 163839 164386

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